Abstract
The genome of Saccharomyces cerevisiae encodes three close homologues of the Escherichia coli 2'-O-rRNA methyltransferase FtsJ/RrmJ, designated Trm7p, Spb1p and Mrm2p. We present evidence that Trm7p methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop, both in vivo and in vitro. In a trm7Delta strain, which is viable but grows slowly, translation is impaired, thus indicating that these tRNA modifications could be important for translation efficiency. We discuss the emergence of a family of three 2'-O-RNA methyltransferases in Eukaryota and one in Prokaryota from a common ancestor. We propose that each eukaryotic enzyme is located in a different cell compartment, in which it would methylate a different RNA that can adopt a very similar secondary structure.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Anticodon / metabolism*
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Base Sequence
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Catalysis
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Cell Cycle Proteins / chemistry
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Escherichia coli / enzymology
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Eukaryotic Cells
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Humans
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Methyltransferases / chemistry
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Molecular Sequence Data
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Mutagenesis
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Nucleic Acid Conformation
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Protein Biosynthesis
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Protein Structure, Tertiary
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RNA, Fungal / metabolism*
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RNA, Messenger
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RNA, Transfer / metabolism*
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / classification
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Ribose / analogs & derivatives
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Ribose / biosynthesis*
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S-Adenosylmethionine / metabolism
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae Proteins / chemistry
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Saccharomyces cerevisiae Proteins / classification
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
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Sequence Homology, Amino Acid
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tRNA Methyltransferases / chemistry
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tRNA Methyltransferases / classification
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tRNA Methyltransferases / genetics
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tRNA Methyltransferases / metabolism*
Substances
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Anticodon
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Cell Cycle Proteins
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RNA, Fungal
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RNA, Messenger
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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D-2-O-methylribose
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Ribose
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S-Adenosylmethionine
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RNA, Transfer
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Methyltransferases
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Trm7 protein, S cerevisiae
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rlmE protein, E coli
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tRNA Methyltransferases