Abstract
Cdt1 is essential for loading Mcm2-7 proteins into prereplicative complexes (pre-RCs) during replication licensing and has been found in organisms as diverse as fission yeast and humans. We have identified a homologue of Cdt1 in Saccharomyces cerevisiae, which is required for pre-RC assembly. We show that, like Mcm2-7p, Cdt1p accumulates in the nucleus during G1 phase and is excluded from the nucleus later in the cell cycle by cyclin dependent kinases (cdks). Cdt1p interacts with the Mcm2--7p complex, and the nuclear accumulation of these proteins during G1 is interdependent. This coregulation of Cdt1p and Mcm2-7p represents a novel level of pre-RC control.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism*
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Cell Nucleus / metabolism
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Chromosomal Proteins, Non-Histone
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Cyclin-Dependent Kinases / antagonists & inhibitors
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DNA Replication
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Fungal Proteins / metabolism
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G1 Phase / drug effects
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Humans
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Hydroxyurea / pharmacology
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Macromolecular Substances
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Minichromosome Maintenance Complex Component 7
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Molecular Sequence Data
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Nuclear Proteins / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae / cytology*
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Saccharomyces cerevisiae / drug effects
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / metabolism*
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Sequence Homology, Amino Acid
Substances
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CDC6 protein, S cerevisiae
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CDT1 protein, human
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Cell Cycle Proteins
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Chromosomal Proteins, Non-Histone
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DNA-Binding Proteins
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Fungal Proteins
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Macromolecular Substances
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Nuclear Proteins
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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Cyclin-Dependent Kinases
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MCM2 protein, S cerevisiae
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MCM7 protein, S cerevisiae
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MCM7 protein, human
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Minichromosome Maintenance Complex Component 7
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Hydroxyurea