Abstract
Dissection of complex processes using model organisms such as yeasts relies heavily upon the use of conditional mutants. We have generated a collection of fission yeast mutants sensitive to dimethylsulphoxide (DMSO). Among these we have found a mutant in the Schizosaccharomyces pombe orthologue of the Saccharomyces cerevisiae SEC13 gene, which fails to cleave the division septum. Generation of a null allele demonstrates that the S. pombe sec13 gene is essential.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Cell Division / drug effects
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Cell Division / genetics
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Cell Survival / genetics
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Cloning, Molecular
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Dimethyl Sulfoxide / pharmacology*
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Genes, Essential / genetics
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Membrane Proteins / chemistry
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Molecular Sequence Data
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Mutagenesis / genetics
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Mutation / drug effects
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Mutation / genetics*
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Nuclear Pore Complex Proteins
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins
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Schizosaccharomyces / cytology*
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Schizosaccharomyces / drug effects
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Schizosaccharomyces / genetics*
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Schizosaccharomyces / metabolism
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Schizosaccharomyces pombe Proteins / chemistry
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Schizosaccharomyces pombe Proteins / genetics
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Schizosaccharomyces pombe Proteins / metabolism
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Time Factors
Substances
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Membrane Proteins
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Nuclear Pore Complex Proteins
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SEC13 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Schizosaccharomyces pombe Proteins
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Dimethyl Sulfoxide