Budding yeast Dsk2p is a polyubiquitin-binding protein that can interact with the proteasome

Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):745-50. doi: 10.1073/pnas.012585199.

Abstract

Dsk2p from Saccharomyces cerevisiae belongs to the class of proteins that contain a ubiquitin-like (UbL) domain at the N terminus together with a ubiquitin-associated (UBA) domain at the C terminus. We show here that the C-terminal UBA domain of Dsk2p binds to K48-linked polyubiquitin chains, and the N-terminal UbL domain of Dsk2p interacts with the proteasome. Overexpression of Dsk2p caused the accumulation of large amounts of polyubiquitin, and extragenic suppressors of the Dsk2p-mediated lethality proved to be temperature-sensitive mutations in two proteasome subunits, rpn1 and pre2. K48-linked ubiquitin-dependent degradation was impaired by disruption of the DSK2 gene. These results indicate that Dsk2p is K48-linked polyubiquitin-binding protein and also interacts with the proteasome. We discuss a possible role of adaptor function of Dsk2p via its UbL and UBA domains in the ubiquitin-proteasome pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Cell Cycle Proteins*
  • Cysteine Endopeptidases / chemistry
  • Cysteine Endopeptidases / genetics
  • Cysteine Endopeptidases / metabolism*
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Genes, Fungal
  • Genes, Suppressor
  • Multienzyme Complexes / chemistry
  • Multienzyme Complexes / genetics
  • Multienzyme Complexes / metabolism*
  • Mutation
  • Proteasome Endopeptidase Complex
  • Protein Structure, Tertiary
  • Protein Subunits
  • Proteins / chemistry
  • Proteins / genetics
  • Proteins / metabolism
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Saccharomyces cerevisiae Proteins / chemistry
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Temperature
  • Two-Hybrid System Techniques
  • Ubiquitins / chemistry
  • Ubiquitins / genetics
  • Ubiquitins / metabolism*

Substances

  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • DSK2 protein, S cerevisiae
  • Fungal Proteins
  • Multienzyme Complexes
  • Protein Subunits
  • Proteins
  • RAD23 protein, S cerevisiae
  • RPN1 protein, S cerevisiae
  • Recombinant Proteins
  • Saccharomyces cerevisiae Proteins
  • Ubiquitins
  • Cysteine Endopeptidases
  • PRE2 protein, S cerevisiae
  • Proteasome Endopeptidase Complex