Abstract
In fission yeast, nutrient starvation induces physiological, biochemical, and morphological changes that enable survival. Collectively these changes are referred to as stationary phase. We have used a green fluorescent protein random insertional mutagenesis system to isolate two novel stress-response proteins required in stationary phase. Ish1 is a nuclear envelope protein that is present throughout the cell cycle and whose expression is increased in response to stresses such as glucose and nitrogen starvation, as well as osmotic stress. Expression of Ish1 is regulated by the Spc1 MAPK pathway through the Atf1 transcription factor. Although overexpression of Ish1 is lethal, cells lacking ish1 exhibit reduced viability in stationary phase. Bis1 is a novel interacting partner of Ish1. Bis1 is the Schizosaccharomyces pombe member of the ES2 nuclear protein family found in Mus musculus, Drosophila melanogaster, Homo sapiens, and Arabidopsis thaliana. Overexpression of Bis1 results in a cell elongation phenotype, whereas bis1(-) cells exhibit a reduced viability in stationary phase similar to that seen in ish1(-) cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Activating Transcription Factor 1
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Amino Acid Sequence
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Bacterial Proteins / metabolism
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Carrier Proteins / biosynthesis
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Carrier Proteins / genetics*
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Cell Nucleus / metabolism*
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Cloning, Molecular
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DNA-Binding Proteins*
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Genetic Vectors
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Green Fluorescent Proteins
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Luminescent Proteins / metabolism
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MAP Kinase Signaling System
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Membrane Proteins / biosynthesis
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Membrane Proteins / genetics*
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Microscopy, Fluorescence
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Models, Genetic
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Molecular Sequence Data
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Phenotype
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Polymerase Chain Reaction
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Promoter Regions, Genetic
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Protein Structure, Tertiary
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RNA-Binding Proteins
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Recombinant Fusion Proteins / metabolism
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Schizosaccharomyces / genetics*
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Schizosaccharomyces pombe Proteins / biosynthesis
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Schizosaccharomyces pombe Proteins / genetics*
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Sequence Homology, Amino Acid
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Time Factors
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Transcription Factors / metabolism
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Two-Hybrid System Techniques
Substances
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Activating Transcription Factor 1
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Bacterial Proteins
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Bis1 protein, S pombe
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Carrier Proteins
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DNA-Binding Proteins
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Luminescent Proteins
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Membrane Proteins
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RNA-Binding Proteins
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Recombinant Fusion Proteins
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Schizosaccharomyces pombe Proteins
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Transcription Factors
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ish1 protein, S pombe
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yellow fluorescent protein, Bacteria
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Green Fluorescent Proteins