Abstract
Rho proteins are down-regulated in vivo by specific GTPase activating proteins (RhoGAP). We have functionally studied three Saccharomyces cerevisiae putative RhoGAP. By first identifying Rho partners with a systematic two-hybrid approach and then using an in vitro assay, we have demonstrated that the Bag7 protein stimulated the GTPase activity of the Rho1 protein, Lrg1p acted on the Cdc42 and Rho2 GTPases and we showed that Rgd2p has a GAP activity on both Cdc42p and Rho5p. In addition, we brought the first evidence for the existence of a sixth functional Rho in yeast, the Cdc42/Rac-like GTPase Rho5.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Fungal Proteins / chemistry
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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GTPase-Activating Proteins / chemistry
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GTPase-Activating Proteins / genetics
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GTPase-Activating Proteins / metabolism*
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Genes, Reporter / genetics
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Molecular Sequence Data
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae / physiology*
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Saccharomyces cerevisiae Proteins*
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Sequence Alignment
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Two-Hybrid System Techniques
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rho GTP-Binding Proteins / chemistry
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rho GTP-Binding Proteins / genetics
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rho GTP-Binding Proteins / metabolism*
Substances
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Bag7 protein, S cerevisiae
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DNA-Binding Proteins
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Fungal Proteins
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GTPase-Activating Proteins
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LRG1 protein, S cerevisiae
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Recombinant Fusion Proteins
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Rgd2 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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rho GTP-Binding Proteins