Abstract
Cvt19 is specifically required for the transport of resident vacuolar hydrolases that utilize the cytoplasm-to-vacuole targeting (Cvt) pathway. Autophagy (Apg) and pexophagy, processes that use the majority of the same protein components as the Cvt pathway, do not require Cvt19. Cvt19GFP is localized to punctate structures on or near the vacuole surface. Cvt19 is a peripheral membrane protein that binds to the precursor form of the Cvt cargo protein aminopeptidase I (prAPI) and travels to the vacuole with prAPI. These results suggest that Cvt19 is a receptor protein for prAPI that allows for the selective transport of this protein by both the Cvt and Apg pathways.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aminopeptidases / metabolism
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Autophagy / physiology*
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Carrier Proteins / metabolism*
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Cytoplasm / metabolism*
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Mutation / physiology
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Plasmids
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Protein Binding / physiology
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Protein Precursors / metabolism
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins*
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Vacuoles / metabolism*
Substances
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Carrier Proteins
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Fungal Proteins
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Membrane Proteins
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Protein Precursors
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Saccharomyces cerevisiae Proteins
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Aminopeptidases
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APE1 protein, S cerevisiae