Abstract
In Saccharomyces cerevisiae, Cdc13 has been proposed to mediate telomerase recruitment at telomere ends. Stn1, which associates with Cdc13 by the two-hybrid interaction, has been implicated in telomere maintenance. Ten1, a previously uncharacterized protein, was found to associate physically with both Stn1 and Cdc13. A binding defect between Stn1-13 and Ten1 was responsible for the long telomere phenotype of stn1-13 mutant cells. Moreover, rescue of the cdc13-1 mutation by STN1 was much improved when TEN1 was simultaneously overexpressed. Several ten1 mutations were found to confer telomerase-dependent telomere lengthening. Other, temperature-sensitive, mutants of TEN1 arrested at G(2)/M via activation of the Rad9-dependent DNA damage checkpoint. These ten1 mutant cells were found to accumulate single-stranded DNA in telomeric regions of the chromosomes. We propose that Ten1 is required to regulate telomere length, as well as to prevent lethal damage to telomeric DNA.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles
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Cell Cycle
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Cell Cycle Proteins / metabolism
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Chromosomal Proteins, Non-Histone / genetics
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Chromosomal Proteins, Non-Histone / isolation & purification
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Chromosomal Proteins, Non-Histone / metabolism*
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Cyclin B / genetics
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Cyclin B / metabolism*
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DNA Damage
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / isolation & purification
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DNA-Binding Proteins / metabolism*
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Flow Cytometry
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Fungal Proteins / genetics
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Fungal Proteins / isolation & purification
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Fungal Proteins / metabolism*
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Genes, Essential / genetics
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Models, Biological
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Mutation / genetics
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Phenotype
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Precipitin Tests
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Protein Binding
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Saccharomyces cerevisiae / cytology
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / isolation & purification
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Saccharomyces cerevisiae Proteins / metabolism*
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Telomerase / metabolism
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Telomere / genetics
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Telomere / metabolism*
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Two-Hybrid System Techniques
Substances
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Cell Cycle Proteins
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Chromosomal Proteins, Non-Histone
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Cyclin B
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DNA-Binding Proteins
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Fungal Proteins
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Saccharomyces cerevisiae Proteins
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TEN1 protein, S cerevisiae
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rad9 protein
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Telomerase