Abstract
Yeast Smt3 is a ubiquitin-like protein similar to the mammalian SUMO-1. Cdc3, a septin component, is known to be modified by Smt3. The level of this modification was affected by Smt3-specific protease mutation ulp1-ts or overexpression of ULP1. By two-hybrid screening, we isolated 5 UIP (Ulp1 interacting protein) genes. UIP1 was identical to NUP42 encoding a component of the nuclear pore complex (NPC). Gle1, another NPC-associating component, also interacted with Ulp1 in the two-hybrid system and co-immunoprecipitation experiment. Thus Ulp1 associates with nucleoporins and may interact with septin rings in the telophase.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carrier Proteins / metabolism
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Cell Cycle Proteins
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Cysteine Endopeptidases / metabolism*
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Fungal Proteins / metabolism
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Nuclear Pore / metabolism
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Nuclear Pore Complex Proteins*
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Nuclear Proteins / metabolism*
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Porins / metabolism
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Profilins
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RNA-Binding Proteins*
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Saccharomyces cerevisiae Proteins*
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Yeasts
Substances
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AGFG1 protein, human
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CDC3 protein, S cerevisiae
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Carrier Proteins
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Cell Cycle Proteins
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Fungal Proteins
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GLE1 protein, S cerevisiae
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Nuclear Pore Complex Proteins
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Nuclear Proteins
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Porins
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Profilins
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RNA-Binding Proteins
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Saccharomyces cerevisiae Proteins
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Cysteine Endopeptidases
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Ulp1 protease