Abstract
Asymmetric localization of proteins plays a key role in many cellular processes, including cell polarity and cell fate determination. Using DNA microarray analysis, we identified a plasma membrane protein-encoding mRNA (IST2) that is transported to the bud tip by an actomyosin-based process. mRNA localization created a higher concentration of IST2 protein in the bud compared with that of the mother cell, and this asymmetry was maintained by a septin-mediated membrane diffusion barrier at the mother-bud neck. These results indicate that yeast creates distinct plasma membrane compartments, as has been described in neurons and epithelial cells.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Actomyosin / metabolism
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Biological Transport
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Cell Compartmentation
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Cell Cycle
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism*
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Cell Membrane / metabolism
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Cytoskeletal Proteins*
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DNA-Binding Proteins*
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Diffusion
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Mutation
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Myosin Heavy Chains*
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Myosin Type V*
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Myosins / metabolism
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Oligonucleotide Array Sequence Analysis
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RNA, Fungal / metabolism
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RNA, Messenger / metabolism*
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Recombinant Fusion Proteins / metabolism
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Repressor Proteins*
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Saccharomyces cerevisiae / cytology
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins*
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Temperature
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Transcription Factors / genetics
Substances
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ASH1 protein, S cerevisiae
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CDC12 protein, S cerevisiae
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Cell Cycle Proteins
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Cytoskeletal Proteins
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DNA-Binding Proteins
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Fungal Proteins
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Membrane Proteins
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Myo4 protein, S cerevisiae
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RNA, Fungal
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RNA, Messenger
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Recombinant Fusion Proteins
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Repressor Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Actomyosin
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Myosin Type V
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Myosin Heavy Chains
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Myosins