Abstract
Ypt/Rab proteins of the Ras superfamily are regulators of protein transport in exo- and endocytosis. Like Ras and Rho proteins, they have a slow intrinsic GTPase activity that can be accelerated by several orders of magnitude by GTPase-activating proteins (GAP). Here we describe a new member of a family of Ypt/Rab-specific GAPs, Msb4p/Gyp4p, that shares with other Gyp family members significant homology in the catalytic domain, recently identified in Gyp1p and Gyp7p. Purified Msb4p/Gyp4p acts primarily on Sec4p, Ypt6p and Ypt7p and might have a role in polarized secretion.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / metabolism*
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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Cytoskeleton / metabolism*
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DNA Primers
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Fungal Proteins / chemistry
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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GTPase-Activating Proteins / metabolism*
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Intracellular Signaling Peptides and Proteins
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Molecular Sequence Data
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Saccharomyces cerevisiae Proteins*
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Sequence Homology, Amino Acid
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cdc42 GTP-Binding Protein, Saccharomyces cerevisiae / metabolism*
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rab GTP-Binding Proteins / metabolism*
Substances
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Actins
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DNA Primers
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Fungal Proteins
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GTPase-Activating Proteins
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Intracellular Signaling Peptides and Proteins
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MSB2 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
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rab GTP-Binding Proteins