Abstract
In Caenorhabditis elegans, the gonad acquires two U-shaped arms by the directed migration of its distal tip cells (DTCs) along the body wall basement membranes. Correct migration of DTCs requires the mig-17 gene, which encodes a member of the metalloprotease-disintegrin protein family. The MIG-17 protein is secreted from muscle cells of the body wall and localizes in the basement membranes of gonad. This localization is dependent on the disintegrin-like domain of MIG-17 and its catalytic activity. These results suggest that the MIG-17 metalloprotease directs migration of DTCs by remodeling the basement membrane.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Basement Membrane / enzymology
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Caenorhabditis elegans / cytology
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Caenorhabditis elegans / enzymology*
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans / growth & development
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Caenorhabditis elegans Proteins*
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Cell Movement
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Cloning, Molecular
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Disintegrins / chemistry
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Disintegrins / genetics
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Disintegrins / metabolism*
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Extracellular Matrix / metabolism*
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Gene Expression Profiling
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Genes, Helminth
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Glycosylation
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Gonads / cytology
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Gonads / enzymology
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Gonads / growth & development
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Metalloendopeptidases / chemistry
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Metalloendopeptidases / genetics
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Metalloendopeptidases / metabolism*
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Molecular Sequence Data
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Muscles / cytology
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Muscles / enzymology
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Mutation
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Protein Structure, Tertiary
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / metabolism
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Sequence Alignment
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Transgenes
Substances
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Caenorhabditis elegans Proteins
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Disintegrins
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MIG-17 protein, C elegans
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Recombinant Fusion Proteins
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GON-1 protein, C elegans
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Metalloendopeptidases