Characterization of fission yeast meiotic mutants based on live observation of meiotic prophase nuclear movement

Chromosoma. 2000;109(1-2):103-9. doi: 10.1007/s004120050417.

Abstract

We characterized four meiotic mutants of the fission yeast Schizosaccharomyces pombe by live observation of nuclear movement. Nuclei were stained with either the DNA-specific fluorescent dye Hoechst 33342 or jellyfish green fluorescent protein (GFP) fused with the N-terminal portion of DNA polymerase alpha. We first followed nuclear dynamics in wild-type cells to determine the temporal sequence of meiotic events: nuclear fusion in the conjugated zygote is immediately followed by oscillatory nuclear movements that continue for 146 min; then, after coming to rest, the nucleus remains in the center of the cell for 26 min before the first meiotic division. Next we examined nuclear dynamics in four meiotic mutants: mei1 (also called mat2), mei4, dhc1, and taz1. Mei1 and mei4 both arrest during meiotic prophase; our observations, however, show that the timing of mei1 arrest is quite different from that of mei4: the mei1 mutant arrests after nuclear fusion but before starting the oscillatory nuclear movements, while the mei4 mutant arrests after the nucleus has completed the oscillatory movements but before the first meiotic division. We also show examples of the dynamic phenotypes of dhc1 and taz1, both of which complete meiosis but exhibit impaired nuclear movement and reduced frequencies of homologous recombination: the dhc1 mutant exhibits no nuclear movement after nuclear fusion, while the taz1 mutant exhibits severely impaired nuclear movement after nuclear fusion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Outer Membrane Proteins / genetics
  • Benzimidazoles / metabolism
  • Cell Division
  • Cell Nucleus / genetics*
  • Cell Nucleus / metabolism
  • Cell Nucleus / ultrastructure
  • Chromatin / metabolism
  • Chromosomes / genetics
  • DNA, Fungal / metabolism
  • Dyneins / genetics
  • Escherichia coli Proteins*
  • Gene Deletion
  • Green Fluorescent Proteins
  • Haploidy
  • Luminescent Proteins / genetics
  • Meiosis / genetics*
  • Microscopy, Video
  • Mutation
  • Phosphoprotein Phosphatases*
  • Prophase / genetics*
  • Protein Kinases*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Schizosaccharomyces / cytology
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces pombe Proteins
  • Spindle Apparatus / genetics
  • Telomere-Binding Proteins

Substances

  • Bacterial Outer Membrane Proteins
  • Benzimidazoles
  • Chromatin
  • DNA, Fungal
  • Escherichia coli Proteins
  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • Schizosaccharomyces pombe Proteins
  • Taz1 protein, E coli
  • Telomere-Binding Proteins
  • taz1 protein, S pombe
  • Green Fluorescent Proteins
  • Protein Kinases
  • Phosphoprotein Phosphatases
  • Dyneins
  • bisbenzimide ethoxide trihydrochloride