Abstract
We have isolated the Schizosaccharomyces pombe orthologue of the Saccharomyces cerevisiae MOB1 gene in a screen designed to enrich for septation mutants. The gene is essential, and cells lacking it display a phenotype typical of septation signalling network mutants. mob1p is located on both spindle pole bodies throughout mitosis. In addition it is also co-localised with the medial ring later in mitosis, and flanks the septum as the medial ring contracts. We also demonstrate that mob1p can be precipitated from cells in a complex with the septation regulating kinase sid2p.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles
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Cell Cycle / physiology
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Cell Cycle Proteins / analysis
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Cell Cycle Proteins / genetics*
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Cell Membrane / physiology*
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Cold Temperature
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DNA-Binding Proteins*
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Gene Expression Regulation, Fungal / physiology
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Mitosis / physiology*
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Mutation
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Phosphoproteins / analysis
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Phosphoproteins / genetics*
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Protein Kinases / genetics
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Protein Kinases / metabolism
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Saccharomyces / cytology*
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Saccharomyces / genetics*
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Saccharomyces cerevisiae Proteins*
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Schizosaccharomyces / cytology*
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Schizosaccharomyces / genetics*
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Schizosaccharomyces pombe Proteins*
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Signal Transduction / physiology*
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Spindle Apparatus / physiology*
Substances
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Cell Cycle Proteins
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DNA-Binding Proteins
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MOB1 protein, S cerevisiae
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Phosphoproteins
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Saccharomyces cerevisiae Proteins
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Schizosaccharomyces pombe Proteins
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TAH11 protein, S cerevisiae
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cdc16 protein, S pombe
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cdt1 protein, S pombe
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Protein Kinases
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Sid2 protein, S pombe