Abstract
The human cullin protein CUL-2 functions in a ubiquitin-ligase complex with the von Hippel-Lindau (VHL) tumour suppressor protein. Here we show that, in Caenorhabditis elegans, cul-2 is expressed in proliferating cells and is required at two distinct points in the cell cycle, the G1-to-S-phase transition and mitosis. cul-2 mutant germ cells undergo a G1-phase arrest that correlates with accumulation of CKI-1, a member of the CIP/KIP family of cyclin-dependent-kinase inhibitors. In cul-2 mutant embryos, mitotic chromosomes are unable to condense, leading to unequal DNA segregation, chromosome bridging and the formation of multiple nuclei.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Caenorhabditis elegans / cytology*
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Caenorhabditis elegans / embryology
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans / metabolism
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Caenorhabditis elegans Proteins*
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Cell Cycle Proteins / chemistry
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism*
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Cell Lineage
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Cell Nucleus / genetics
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Chromosomes / chemistry
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Chromosomes / genetics
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Chromosomes / metabolism*
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Cloning, Molecular
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Cullin Proteins*
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Cyclin-Dependent Kinase Inhibitor Proteins
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Fluorescent Antibody Technique
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G1 Phase*
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Gene Expression Regulation, Developmental
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Germ Cells / cytology
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Germ Cells / metabolism
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In Situ Hybridization
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Mitosis*
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Molecular Conformation
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Molecular Sequence Data
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Mutation / genetics
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Nondisjunction, Genetic
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Phenotype
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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S Phase*
Substances
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CUL2 protein, human
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Caenorhabditis elegans Proteins
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Cell Cycle Proteins
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Cullin 1
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Cullin Proteins
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Cyclin-Dependent Kinase Inhibitor Proteins
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RNA, Messenger
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cki-1 protein, C elegans
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cki-2 protein, C elegans
Associated data
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GENBANK/AF179358
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GENBANK/AF179359