The karyopherin Kap122p/Pdr6p imports both subunits of the transcription factor IIA into the nucleus

J Cell Biol. 1999 Oct 18;147(2):235-46. doi: 10.1083/jcb.147.2.235.

Abstract

We discovered a nuclear import pathway mediated by the product of the previously identified Saccharomyces cerevisiae gene PDR6 (pleiotropic drug resistance). This gene product functions as a karyopherin (Kap) for nuclear import. Consistent with previously proposed nomenclature, we have renamed this gene KAP122. Kap122p was localized both to the cytoplasm and the nucleus. As a prominent import substrate of Kap122p, we identified the complex of the large and small subunit (Toa1p and Toa2p, respectively) of the general transcription factor IIA (TFIIA). Recombinant GST-Kap122p formed a complex with recombinant His(6)-Toa1p/Toa2p. In wild-type cells, Toa1p and Toa2p were localized to the nucleus. Consistent with Kap122p being the principal Kap for import of the Toa1p-Toa2p complex, we found that deletion of KAP122 results in increased cytoplasmic localization of both Toa1p and Toa2p. Deletion of KAP122 is not lethal, although deletion of TOA1 and TOA2 is. Together these data suggest that Kap122p is the major Kap for the import of Toa1p-Toa2p into the nucleus. Like other substrate-Kap complexes, the Toa1p/Toa2p/Kap122p complex isolated from yeast cytosol or reconstituted from recombinant proteins, was dissociated by RanGTP but not RanGDP. Kap122p bound to nucleoporins, specifically, to the peptide repeat-containing fragments of Nup1p and Nup2p.

MeSH terms

  • Biological Transport
  • Cell Nucleus / metabolism*
  • DNA-Binding Proteins / metabolism*
  • Immunohistochemistry
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae / ultrastructure
  • Transcription Factor AP-2
  • Transcription Factors / metabolism*
  • beta Karyopherins

Substances

  • DNA-Binding Proteins
  • Nuclear Proteins
  • Recombinant Proteins
  • Transcription Factor AP-2
  • Transcription Factors
  • beta Karyopherins