Abstract
We have isolated a new gene, psu1(+), from the fission yeast Schizosaccharomyces pombe. The predicted amino acid sequences shows that this protein has striking homology to the SUN family of the budding yeast, hence designated Psu1 (S. pombe homologue of the SUN family). Disruption of the psu1(+) gene revealed that it is essential for growth, and the null phenotype showed the swelling of cells followed by eventual lysis. We introduced psu1(+) gene in the disruptant strain and repressed it giving resistance to 1, 3-beta-glucanase digestion. Our results suggest that Psu1 plays an essential role in cell wall synthesis in S. pombe.
Copyright 1999 Academic Press.
MeSH terms
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Amino Acid Sequence
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Antifungal Agents / pharmacology
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Antineoplastic Agents / pharmacology
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Base Sequence
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Cell Wall / metabolism*
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Drug Resistance, Microbial
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Enzyme Activation
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Genes, Essential
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Genes, Fungal*
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Genes, Lethal
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Glucosyltransferases / antagonists & inhibitors
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Glucosyltransferases / metabolism
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Heat-Shock Proteins / genetics
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Membrane Proteins*
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Mitochondrial Proteins
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Multigene Family
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Repressor Proteins / genetics
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Restriction Mapping
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Saccharomyces cerevisiae Proteins*
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Schizosaccharomyces / genetics*
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Schizosaccharomyces pombe Proteins*
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Sequence Homology, Amino Acid
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Trans-Activators
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Zearalenone / analogs & derivatives
Substances
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Antifungal Agents
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Antineoplastic Agents
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Heat-Shock Proteins
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Membrane Proteins
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Mitochondrial Proteins
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Repressor Proteins
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Saccharomyces cerevisiae Proteins
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Schizosaccharomyces pombe Proteins
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Sui1 protein, S pombe
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Trans-Activators
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UTH1 protein, S cerevisiae
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hypothemycin
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Zearalenone
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Glucosyltransferases
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1,3-beta-glucan synthase