Induction of vacuolar Ca2+-ATPase and H+/Ca2+ exchange activity in yeast mutants lacking Pmr1, the Golgi Ca2+-ATPase

FEBS Lett. 1999 Jul 9;454(3):181-6. doi: 10.1016/s0014-5793(99)00803-0.

Abstract

We have analyzed Ca2+ transport activity in defined subcellular fractions of an isogenic set of wild-type and mutant yeast. The results, together with measurements of polypeptide expression levels and promoter::reporter gene activity, show that the Golgi Ca2+-ATPase, Pmr1, is the major Ca2+ pump under normal growth conditions. In the absence of Pmr1, we show a massive, calcineurin-dependent compensatory induction of the vacuolar Ca2+-ATPase, Pmc1. In addition, H+/Ca2+ exchange activity, that may be distinct from the vacuolar exchanger Vcx1, is also increased.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • ATP-Binding Cassette Transporters / genetics
  • ATP-Binding Cassette Transporters / metabolism*
  • Calcium / metabolism
  • Calcium-Transporting ATPases / metabolism*
  • Golgi Apparatus / metabolism
  • Hydrogen / metabolism
  • Ion Transport
  • Molecular Chaperones
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae / ultrastructure
  • Saccharomyces cerevisiae Proteins*

Substances

  • ATP-Binding Cassette Transporters
  • Molecular Chaperones
  • SSC1 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Hydrogen
  • Calcium-Transporting ATPases
  • Calcium