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Table S1. Summary and convergence of data obtained from the four technologies.

A. Protein purification

Step	Number
Heterozygous diploid	81
Haploid with tagged copy of gene as only copy of gene	60
Tagged protein of expected size expressed in logarithmic cultures	53
Tagged protein could be purified and analyzed by mass spectrometry	47
Uncharacterized protein purified with other proteins	37
Function could be predicted from copurifying proteins	32

B. Two-hybrid analysis

Step	Number
Two-hybrid baits constructed	57
Two-hybrid array screens with unchracterized bait	57
Two-hybrid array screens with positives	43
Total two-hybrid positives from array screens	271

C. Protein localization

Step	Number
Heterozygous diploid	89
Haploid with tagged copy of gene as only copy of gene	76
Fluorescent fusion protein detected	64
Localization1:
Nucleus	32
Nucleolus	13
Kinetochore	2
Mitochondrion	8
Nucleus and Mitochondrion	2
Nuclear membrane	2
Nuclear envelope and endoplasmic reticulum	11
Golgi	1
Plasma membrane	1
Cytoplasm	17
Vacuole	1

¹A protein may have more than one localization

D. Protein structure prediction

Step	Domains	ORFs
PSI-blast	40	29
Fold recognition	34	28
Ab initio prediction	51	19
Structures with significant match	16	5
Structures w/o significant match	38	14
Total	133	76

E. Convergence of technologies

Number of Methods	Number of ORFs described
1	19
2	21
3	33
4	23

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