Protein Overview: RPA49

General Information:
Name(s) found:	RPA49 / YNL248C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324081\|ref\|NP_014151.1\| RNA polymerase I subunit A49; Rpa49p [Saccharomyces cerevisiae] [NCBI NR] gi\|45269944\|gb\|AAS56353.1\| YNL248C [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S63221 DNA-directed RNA polymerase (EC 2.7.7.6) A chain RPA49 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1710654\|sp\|Q01080.2\|RPA49_YEAST RecName: Full=DNA-directed RNA polymerase I subunit RPA49; Short=A49; AltName: Full=DNA-directed RNA polymerase I 49 kDa polypeptide [NCBI NR] gi\|1302289\|emb\|CAA96155.1\| RPA49 [Saccharomyces cerevisiae] [NCBI NR] gi\|1255970\|emb\|CAA65496.1\| RPA49 [Saccharomyces cerevisiae] [NCBI NR] DNA-directed RNA polymerase I subunit RPA49 OS=Saccharomyces cerevisiae GN=RPA49 PE=1 SV=2 [Swiss-Prot] DNA-directed RNA polymerase I 49 kDa polypeptide (EC 2.7.7.6) (A49) - Saccharomyces cerevisiae (Baker's yeast) [Swiss-Prot] RPA49 DNA-directed RNA polymerase A (I) chain, 46 kDa [mips] RNA polymerase I subunit A49 [SGD] Chr XIV from 181360-182607, reverse complement [yeastorf3.fasta] Chr XIV from 182607-181360, reverse complement [yeastorf5.fasta] (Q01080) DNA-directed RNA polymerase I 49 kDa polypeptide (EC 2.7.7.6) (A49) [4932.FASTACCERV.txt] RPA49, Chr XIV from 181360-182607, reverse complement [yeast_orfs2] RPA49 SGDID:S0005192, Chr XIV from 182607-181360, reverse complement, Verified ORF [yeastflysequest.fasta] RNA polymerase I subunit A49 [Saccharomyces cerevisiae]gi\|1255970\|emb\|CAA65496.1\| RPA49 [Saccharomyces cerevisiae]gi\|1302289\|emb\|CAA96155.1\| RPA49 [Saccharomyces cerevisiae]gi\|45269944\|gb\|AAS56353.1\| YNL248C [Saccharomyces cerevisiae]gi\|2131237\|pir\|\|S [nrdb_11152004_Chlamydomonas] RPA49 SGDID:S000005192, Chr XIV from 182609-181362, reverse complement, Verified ORF, "RNA polymerase I subunit A49" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] DNA-directed RNA polymerase I 49 kDa polypeptide (A49) [nr-271106-contam.fasta] RNA polymerase I subunit A49; Rpa49p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YNL248C [Saccharomyces cerevisiae] [nr-271106-contam.fasta] RPA49 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] DNA-directed RNA polymerase I subunit RPA49 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPA49 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RPA49 SGDID:S000005192, Chr XIV from 182608-181361, Genome Release 64-1-1, reverse complement, Verified ORF, "RNA polymerase I subunit A49" [yeast-030211-contam.fasta] RPA49 SGDID:S000005192, Chr XIV from 182608-181361, Genome Release 64-2-1, reverse complement, Verified ORF, "RNA polymerase I subunit A49; essential for nucleolar assembly and for high polymerase loading rate; required for nucleolar localization of Rpa34p" [20141208_orf_trans.fasta] TR5297\|c0_g2_i1\|g.3675 ORF TR5297\|c0_g2_i1\|g.3675 TR5297\|c0_g2_i1\|m.3675 type:complete len:416 (+) TR5297\|c0_g2_i1:89-1336(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR1114\|c0_g1_i1\|g.750 ORF TR1114\|c0_g1_i1\|g.750 TR1114\|c0_g1_i1\|m.750 type:complete len:416 (+) TR1114\|c0_g1_i1:76-1323(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YNL248C SGDID:S000005192, Chr XIV from 182608-181361, Genome Release 64-1-1, reverse complement, Verified ORF, "RNA polymerase I subunit A49" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_6082\|g.4371 ORF asmbl_6082\|g.4371 asmbl_6082\|m.4371 type:complete len:416 (+) asmbl_6082:75-1322(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_5757\|g.3544 ORF ID=asmbl_5757\|g.3544 ID=asmbl_5757\|m.3544 type:complete len:416 (+) ID=asmbl_5757:72-1319(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_6108\|g.3585 ORF ID=asmbl_6108\|g.3585 ID=asmbl_6108\|m.3585 type:complete len:416 (+) ID=asmbl_6108:75-1322(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	415 amino acids
Gene Ontology:
Cellular Component:	DNA-directed RNA polymerase I complex [TAS]
Biological Process:	transcription from RNA polymerase I promoter [TAS] regulation of cell size [IMP] ribosome biogenesis [RCA]
Molecular Function:	DNA-directed RNA polymerase activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSVKRSVSEI EIESVQDQPS VAVGSFFKGF RAPSDTTFDL YKKKKSEKDE FVLHGENERL  60
   61 EYEGYTDSSS QASNQYVVGL FNPEKKSIQL YKAPVLVSKV VSKSSKNLRG PKIKSKSDTR 120
  121 PSALRNALGE AFGTKKAKKA IADLERNRID SDKLTDSAID IVDSVRTASK DLPTRAQLDE 180
  181 ITSNDRPTPL ANIDATDVEQ IYPIESIIPK KELQFIRVSS ILKEADKEKK LELFPYQNNS 240
  241 KYVAKKLDSL TQPSQMTKLQ LLYYLSLLLG VYENRRVNNK TKLLERLNSP PEILVDGILS 300
  301 RFTVIKPGQF GRSKDRSYFI DPQNEDKILC YILAIIMHLD NFIVEITPLA HELNLKPSKV 360
  361 VSLFRVLGAI VKGATVAQAE AFGIPKSTAA SYKIATMKVP FKLPEMTRRG RGPRR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		FUN12 MAK5 NET1 NOP56 NOP58 RET1 RPA12 RPA135 RPA14 RPA190 RPA34 RPA43 RPA49 RPB10 RPB5 RPB8 RPC10 RPC19 RPC40 RPC53 RPF2 RPL20B RPL5 RPO26 RPS4B, RPS4A STM1
View Details	(MIPS) Mewes HW, et al. (2004)		RPA12 RPA135 RPA14 RPA190 RPA34 RPA43 RPA49 RPB10 RPB5 RPB8 RPC10 RPC19 RPC40 RPO26
View Details	Krogan NJ, et al. (2006)		BDP1 CDC14 COY1 FAR7 MAF1 RET1 RPA12 RPA135 RPA14 RPA190 RPA34 RPA43 RPA49 RPC10 RPC11 RPC17 RPC19 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO31 YDL144C YIR024C
View Details	Gavin AC, et al. (2006)		RPA12 RPA190 RPA43 RPA49 RPC40
View Details	Gavin AC, et al. (2002)		AMS1 ASC1 CDC14 DHH1 FBA1 MAF1 RET1 RPA12 RPA135 RPA190 RPA43 RPA49 RPB10 RPB5 RPB8 RPC11 RPC17 RPC19 RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO26 RPO31 RSC8 SRO7
View Details	Ho Y, et al. (2002)		ACC1 ACH1 ADH2 ADR1 GCD11 GFA1 GND1 GUS1 IDH2 ISA2 MDH1 MET18 NGL2 PYC1 PYC2 RET1 RPA135 RPA190 RPA49 RPB5 RPC19 RPC25 RPC34 RPC40 RPC82 RPN3 RPO26 RPO31 RVS167 SEC27 TBS1 TFP1 TSA2 YFL042C YHR112C
View Details	Qiu et al. (2008)		RPA12 RPA135 RPA14 RPA190 RPA34 RPA43 RPA49 RPB10 RPB5 RPB8 RPC10 RPC19 RPC34 RPC40 RPO26

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	NAF1	No Comments	Hazbun TR, et al. (2003)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	BOI1	Sample bob1 (2nd set) from october 2005	McCusker D, et al (2007)
View Run	MLP2	#19 Asynchronous Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#19b Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#19a Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	RPA49	Huh WK, et al. (2003)
View Data	RPA49 and NOP56	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..415]	deduced	N/A	No confident structure predictions are available.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Protein Overview: RPA49

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: