Protein Overview: HSC82

General Information:
Name(s) found:	HSC82 / YMR186W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|854456\|emb\|CAA89919.1\| Hsc82p [Saccharomyces cerevisiae] [NCBI NR] gi\|6323840\|ref\|NP_013911.1\| Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat [NCBI NR] gi\|1708315\|sp\|P15108.4\|HSC82_YEAST RecName: Full=ATP-dependent molecular chaperone HSC82; AltName: Full=Heat shock protein Hsp90 constitutive isoform; AltName: Full=82 kDa heat shock cognate protein [NCBI NR] pir\|\|S55133 heat shock protein HSC82 - yeast (Saccharomyces cerevisiae) [NCBI NR] ATP-dependent molecular chaperone HSC82 OS=Saccharomyces cerevisiae GN=HSC82 PE=1 SV=4 [Swiss-Prot] HSC82 Heat shock protein [mips] Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock [SGD] HSC82, Chr XIII from 632354-634471 [yeast_orfs2] Chr XIII from 632354-634471 [yeastorf3.fasta] HSC82 SGDID:S0004798, Chr XIII from 632354-634471, Verified ORF [yeastflysequest.fasta] Hsc82p [Saccharomyces cerevisiae]gi\|854456\|emb\|CAA89919.1\| Hsc82p [Saccharomyces cerevisiae]gi\|1708315\|sp\|P15108\|HS83_YEAST Heat shock cognate protein HSC82gi\|1077176\|pir\|\|S55133 heat shock protein HSC82 - yeast (Saccharomyces cerevisiae) [nrdb_11152004_Chlamydomonas] HSC82 SGDID:S000004798, Chr XIII from 632354-634471, Verified ORF, "Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Hsc82p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ATP-dependent molecular chaperone HSC82 (Heat shock protein Hsp90 constitutive isoform) (82 kDa heat shock cognate protein) [nr-271106-contam.fasta] Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels that HSP82 and induced 2-3 fold by heat shock; Hsc82p [Saccharomyce [nr-271106-contam.fasta] HSC82 SGDID:S000004798, Chr XIII from 632354-634471, Verified ORF, "Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock" [yeast-bovrabprots.fasta] HSC82 SGDID:S000004798, Chr XIII from 632354-634471, Verified ORF, "Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels that HSP82 and induced 2-3 fold by heat shock" [yeast-contam_custom.fasta] [chemostatdb.fasta] ATP-dependent molecular chaperone HSC82 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HSC82 PE=1 SV=4 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] HSC82 SGDID:S000004798, Chr XIII from 632355-634472, Genome Release 64-1-1, Verified ORF, "Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock" [yeast-030211-contam.fasta] HSC82 SGDID:S000004798, Chr XIII from 632355-634472, Genome Release 64-2-1, Verified ORF, "Cytoplasmic chaperone of the Hsp90 family; plays a role in determining prion variants; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YMR186W SGDID:S000004798, Chr XIII from 632355-634472, Genome Release 64-1-1, Verified ORF, "Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] ID=asmbl_4281\|g.2488 ORF ID=asmbl_4281\|g.2488 ID=asmbl_4281\|m.2488 type:complete len:706 (+) ID=asmbl_4281:151-2268(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	705 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA] mitochondrion [IDA ]
Biological Process:	telomere maintenance [IMP] protein folding [IMP] protein refolding [ISS] proteasome assembly [IMP][IPI] 'de novo' protein folding [ISS] response to stress [IMP]
Molecular Function:	unfolded protein binding [IDA] ATPase activity, coupled [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAGETFEFQA EITQLMSLII NTVYSNKEIF LRELISNASD ALDKIRYQAL SDPKQLETEP  60
   61 DLFIRITPKP EEKVLEIRDS GIGMTKAELI NNLGTIAKSG TKAFMEALSA GADVSMIGQF 120
  121 GVGFYSLFLV ADRVQVISKN NEDEQYIWES NAGGSFTVTL DEVNERIGRG TVLRLFLKDD 180
  181 QLEYLEEKRI KEVIKRHSEF VAYPIQLLVT KEVEKEVPIP EEEKKDEEKK DEDDKKPKLE 240
  241 EVDEEEEEKK PKTKKVKEEV QELEELNKTK PLWTRNPSDI TQEEYNAFYK SISNDWEDPL 300
  301 YVKHFSVEGQ LEFRAILFIP KRAPFDLFES KKKKNNIKLY VRRVFITDEA EDLIPEWLSF 360
  361 VKGVVDSEDL PLNLSREMLQ QNKIMKVIRK NIVKKLIEAF NEIAEDSEQF DKFYSAFAKN 420
  421 IKLGVHEDTQ NRAALAKLLR YNSTKSVDEL TSLTDYVTRM PEHQKNIYYI TGESLKAVEK 480
  481 SPFLDALKAK NFEVLFLTDP IDEYAFTQLK EFEGKTLVDI TKDFELEETD EEKAEREKEI 540
  541 KEYEPLTKAL KDILGDQVEK VVVSYKLLDA PAAIRTGQFG WSANMERIMK AQALRDSSMS 600
  601 SYMSSKKTFE ISPKSPIIKE LKKRVDEGGA QDKTVKDLTN LLFETALLTS GFSLEEPTSF 660
  661 ASRINRLISL GLNIDEDEET ETAPEASTEA PVEEVPADTE MEEVD

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ADH1 ARO1 CAM1 CPR6 EFT2, EFT1 FBA1 GPM1 HSC82 HSP104 HSP82 HXK1 PDC1 PGI1 SSZ1 STI1 TDH1 TIF1, TIF2 TKL1 TPI1 YEF3 YHR020W
View Details	Krogan NJ, et al. (2006)		AHA1 BUD3 CNS1 CPR6 DIG2 DNM1 DSF1, YNR073C FRT2 GPA2 HOM6 HSC82 HSP104 HSP82 MBB1 MRPL50 PAM1 PPT1 QNS1 RMD8 RTS1 SMT3 STI1 STP4 TDH1 UTP20 YBL104C
View Details	Gavin AC, et al. (2002)		ACT1 ADH1 ARO1 ARX1 BEM2 CCM1 CCT8 CLU1 CMR1 CPA2 DNA2 ECM1 ECM16 EHD3 ENO2 FAR11 FAR3 FBA1 FYV4 GCN1 GCN20 GEP3 GFA1 GPM1 GUS1 HDA1 HDA2 HDA3 HFM1 HSC82 HSP82 IDH2 ILV1 KAP123 KAP95 LCD1 LYS2 MEC1 MGM101 MKT1 MLC1 MLC2 MLH1 MPH1 MRP1 MRP10 MRP13 MRP17 MRP21 MRP4 MRP51 MRPS16 MRPS17 MRPS18 MRPS28 MRPS5 MRPS8 MRPS9 MSH2 MSH3 MSH6 MYO1 MYO2 MYO4 NAM9 NIP1 OLA1 PAP2 PDC1 PET123 PFK1 PGK1 PMS1 PPT1 PRO2 PSD2 PSE1 PUS7 RAD16 RAD51 RAD52 RFA1 RFA2 RFA3 RIM1 RIX1 RNH1 ROM2 RPA135 RPB2 RPN9 RPT3 RPT6 RSC8 RSM10 RSM19 RSM22 RSM23 RSM24 RSM25 RSM26 RSM27 RSM7 RVB2 SGS1 SHE2 SHE3 SHS1 SNT2 SSE2 SXM1 TIF1, TIF2 TIF4631 TOP3 TPI1 UBP10 UTP20 UTP22 VAS1 VPS1 YEF3 YHB1 YHR122W

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ALG13	No Comments	Hazbun TR, et al. (2003)
View Run	PSF2	No Comments	Hazbun TR, et al. (2003)
View Run	UTP23	No Comments	Hazbun TR, et al. (2003)
View Run	SDO1	No Comments	Hazbun TR, et al. (2003)
View Run	TOR1	No Comments	Reinke A, et al. (2004)
View Run	DSN1	No Comments	Hazbun TR, et al. (2003)
View Run	PGA1	No Comments	Hazbun TR, et al. (2003)
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	SEC10	No Comments	De Craene, J., et al. (2006)
View Run	BIR1	No Comments	Widlund PO, et al. (2005)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RLF2	cac1: TAP-tagged	Green EM, et al (2005)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#20 Asynchronous Prep1-TiO2 Phosphopeptide enrichment, PartB	Keck JM, et al. (2011)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#04 Alpha Factor Prep2	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#28 Asynchronous Prep5-TiO2 Phosphopeptide Enrichment	Keck JM, et al. (2011)
View Run	MLP2	#05 Alpha Factor Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#07 Alpha Factor Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#09 Alpha Factor Prep4-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	NSE4	HSC82	2	Unpublished Fields Lab Data
View Screen	PPG1	HSC82	2	Uetz P, et al. (2000)
View Screen	PAC1	HSC82	2	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	HSC82	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..220]	PSI-BLAST	1204.0	HSP90
2	View Details	[221..705]	Pfam	1000.0	Hsp90 protein No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.87

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2