Protein Overview: RRB1

General Information:
Name(s) found:	RRB1 / YMR131C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|728670\|emb\|CAA88556.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] gi\|6323779\|ref\|NP_013850.1\| Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p; Rrb1p [Saccharomyces cerevisiae] [NCBI NR] gi\|2494913\|sp\|Q04225.1\|RRB1_YEAST RecName: Full=Ribosome assembly protein RRB1 [NCBI NR] gi\|1078579\|pir\|\|S53061 hypothetical protein YMR131c - yeast (Saccharomyces cerevisiae) [NCBI NR] Ribosome assembly protein RRB1 OS=Saccharomyces cerevisiae GN=RRB1 PE=1 SV=1 [Swiss-Prot] RRB1 Protein involved in the regulation of ribosome biosynthesis [mips] Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p [SGD] RSA2, Chr XIII from 533162-534697, reverse complement [yeast_orfs2] Chr XIII from 533162-534697, reverse complement [yeastorf3.fasta] Chr XIII from 534697-533162, reverse complement [yeastorf5.fasta] (Q04225) Ribosome assembly protein RRB1 [4932.FASTACCERV.txt] RRB1 SGDID:S0004738, Chr XIII from 534697-533162, reverse complement, Verified ORF [yeastflysequest.fasta] RiboSome Assembly 2 [Saccharomyces cerevisiae]gi\|728670\|emb\|CAA88556.1\| unknown [Saccharomyces cerevisiae]gi\|2494913\|sp\|Q04225\|RRB1_YEAST Ribosome assembly protein RRB1gi\|1078579\|pir\|\|S53061 hypothetical protein YMR131c - yeast (Saccharomyces cerevisia [nrdb_11152004_Chlamydomonas] RRB1 SGDID:S000004738, Chr XIII from 534697-533162, reverse complement, Verified ORF, "Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] unknown [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p; Rrb1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Ribosome assembly protein RRB1 [nr-271106-contam.fasta] [chemostatdb.fasta] Ribosome assembly protein RRB1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RRB1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RRB1 SGDID:S000004738, Chr XIII from 534698-533163, Genome Release 64-1-1, reverse complement, Verified ORF, "Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p" [yeast-030211-contam.fasta] RRB1 SGDID:S000004738, Chr XIII from 534698-533163, Genome Release 64-2-1, reverse complement, Verified ORF, "Nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p; essential gene" [20141208_orf_trans.fasta] YMR131C SGDID:S000004738, Chr XIII from 534698-533163, Genome Release 64-1-1, reverse complement, Verified ORF, "Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	511 amino acids
Gene Ontology:
Cellular Component:	nucleolus [IDA]
Biological Process:	ribosome biogenesis [IMP][IPI][RCA]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSKRSIEVNE EQDRVVSAKT ESHSVPAIPA SEEQDAPKND LEEQLSDEFD SDGEIIEIDG  60
   61 DDEINDEDDL RKKQEEAETL VQKDQSEGNK EKIQELYLPH MSRPLGPDEV LEADPTVYEM 120
  121 LHNVNMPWPC LTLDVIPDTL GSERRNYPQS ILLTTATQSS RKKENELMVL ALSNLAKTLL 180
  181 KDDNEGEDDE EDDEDDVDPV IENENIPLRD TTNRLKVSPF AISNQEVLTA TMSENGDVYI 240
  241 YNLAPQSKAF STPGYQIPKS AKRPIHTVKN HGNVEGYGLD WSPLIKTGAL LSGDCSGQIY 300
  301 FTQRHTSRWV TDKQPFTVSN NKSIEDIQWS RTESTVFATA GCDGYIRIWD TRSKKHKPAI 360
  361 SVKASNTDVN VISWSDKIGY LLASGDDNGT WGVWDLRQFT PSNADAVQPV AQYDFHKGAI 420
  421 TSIAFNPLDE SIVAVGSEDN TVTLWDLSVE ADDEEIKQQA AETKELQEIP PQLLFVHWQK 480
  481 EVKDVKWHKQ IPGCLVSTGT DGLNVWKTIS V

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		AMD1 RRB1 YJL070C
View Details	Qiu et al. (2008)		DRS1 NAN1 NSR1 NUG1 PXR1 RRB1 UTP15 UTP4 UTP5

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#33 Asynchronous SPB prep	Keck JM, et al. (2011)
View Run	MLP2	#19 Asynchronous Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#35 Asynchronous SPB prep	Keck JM, et al. (2011)
View Run	MLP2	#04 Alpha Factor Prep2	Keck JM, et al. (2011)
View Run	MLP2	#06 Alpha Factor Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#19b Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#19a Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RRB1	YGR160W	2	Unpublished Fields Lab Data
View Screen	RRB1	BDS1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	RRB1	Huh WK, et al. (2003)
View Data	RRB1 and NOP56	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..181]	MSA	1.673995	View MSA. No confident structure predictions are available.
2	View Details	[182..511]	PSI-BLAST	87.70927	beta1-subunit of the signal-transducing G protein heterotrimer

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
histone methyltransferase activity (H3-K4 specific)	4.71992275913384	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	2.79445416141967	bayes_pls_golite062009
snoRNA binding	2.62514080753887	bayes_pls_golite062009
binding	2.42570478946173	bayes_pls_golite062009
transcription regulator activity	2.3610959773142	bayes_pls_golite062009
nucleic acid binding	2.30918780727915	bayes_pls_golite062009
transcription repressor activity	2.29049708962212	bayes_pls_golite062009
S-adenosylmethionine-dependent methyltransferase activity	2.18628561734369	bayes_pls_golite062009
histone methyltransferase activity	1.85632145559025	bayes_pls_golite062009
DNA binding	1.71685536470455	bayes_pls_golite062009
dynein binding	1.62099593211487	bayes_pls_golite062009
transcription corepressor activity	1.41752877005447	bayes_pls_golite062009
protein methyltransferase activity	1.35170368882651	bayes_pls_golite062009
acetyltransferase activity	1.23134876981279	bayes_pls_golite062009
protein binding	1.21879977456466	bayes_pls_golite062009
cytoskeletal protein binding	1.19593750149343	bayes_pls_golite062009
lysine N-methyltransferase activity	1.15313682561559	bayes_pls_golite062009
histone-lysine N-methyltransferase activity	1.15313682561559	bayes_pls_golite062009
protein-lysine N-methyltransferase activity	1.15313682561559	bayes_pls_golite062009
transcription factor activity	1.08768505897883	bayes_pls_golite062009
transcription initiation factor activity	1.01962930136588	bayes_pls_golite062009
	0.88172763425403	bayes_pls_golite062009
histone binding	0.685319917508843	bayes_pls_golite062009
RNA polymerase II transcription factor activity	0.676924760416604	bayes_pls_golite062009
DNA-dependent ATPase activity	0.625780732991652	bayes_pls_golite062009
actin binding	0.529861581509437	bayes_pls_golite062009
transcription factor binding	0.489473003915035	bayes_pls_golite062009
transferase activity, transferring acyl groups	0.437810477160064	bayes_pls_golite062009
DNA polymerase activity	0.428715007921149	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	0.426429095658617	bayes_pls_golite062009
kinase activity	0.387572734234845	bayes_pls_golite062009
transcription cofactor activity	0.30761651528651	bayes_pls_golite062009
transporter activity	0.284701931503833	bayes_pls_golite062009
structural molecule activity	0.254012051644049	bayes_pls_golite062009
protein kinase activity	0.229242350006544	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.226335610368636	bayes_pls_golite062009
DNA helicase activity	0.220747580531792	bayes_pls_golite062009
enzyme regulator activity	0.161639372576406	bayes_pls_golite062009
chromatin binding	0.151977098745772	bayes_pls_golite062009
N-methyltransferase activity	0.00335766408304172	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2