Protein Overview: NAM2

General Information:
Name(s) found:	NAM2 / YLR382C [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|SYBYLM leucine-tRNA ligase (EC 6.1.1.4) precursor, mitochondrial - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6323414\|ref\|NP_013486.1\| Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance; Nam2p [Saccharomyces cerevisiae] [NCBI NR] gi\|609429\|gb\|AAB67277.1\| Nam2p: mitochondrial leucyl-tRNA synthetase [Saccharomyces cerevisiae] [NCBI NR] gi\|4021\|emb\|CAA28791.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|172008\|gb\|AAA34805.1\| leucyl-tRNA synthetase [NCBI NR] gi\|135144\|sp\|P11325.1\|SYLM_YEAST RecName: Full=Leucyl-tRNA synthetase, mitochondrial; AltName: Full=Leucine--tRNA ligase; Short=LeuRS; Flags: Precursor [NCBI NR] Leucyl-tRNA synthetase, mitochondrial OS=Saccharomyces cerevisiae GN=NAM2 PE=1 SV=1 [Swiss-Prot] NAM2 Leucine--tRNA ligase precursor, mitochondrial [mips] Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance [SGD] NAM2, Chr XII from 882067-884751, reverse complement [yeast_orfs2] Chr XII from 882067-884751, reverse complement [yeastorf3.fasta] Chr XII from 884751-882067, reverse complement [yeastorf5.fasta] (P11325) Leucyl-tRNA synthetase, mitochondrial precursor (EC 6.1.1.4) (Leucine--tRNA ligase) (LeuRS) [4932.FASTACCERV.txt] NAM2 SGDID:S0004374, Chr XII from 884751-882067, reverse complement, Verified ORF [yeastflysequest.fasta] Nam2p [Saccharomyces cerevisiae]gi\|4021\|emb\|CAA28791.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|68518\|pir\|\|SYBYLM leucine-tRNA ligase (EC 6.1.1.4) precursor, mitochondrial - yeast (Saccharomyces cerevisiae)gi\|609429\|gb\|AAB67277.1\| Nam2p: m [nrdb_11152004_Chlamydomonas] NAM2 SGDID:S000004374, Chr XII from 884751-882067, reverse complement, Verified ORF, "Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenan [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] leucyl-tRNA synthetase [nr-271106-contam.fasta] Leucyl-tRNA synthetase, mitochondrial precursor (Leucine--tRNA ligase) (LeuRS) [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance; Nam2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Nam2p: mitochondrial leucyl-tRNA synthetase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] NAM2 SGDID:S000004374, Chr XII from 884751-882067, reverse complement, Verified ORF, "Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Leucine--tRNA ligase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NAM2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] NAM2 SGDID:S000004374, Chr XII from 884751-882067, Genome Release 64-1-1, reverse complement, Verified ORF, "Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance" [yeast-030211-contam.fasta] NAM2 SGDID:S000004374, Chr XII from 884751-882067, Genome Release 64-2-1, reverse complement, Verified ORF, "Mitochondrial leucyl-tRNA synthetase; also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance" [20141208_orf_trans.fasta] YLR382C SGDID:S000004374, Chr XII from 884751-882067, Genome Release 64-1-1, reverse complement, Verified ORF, "Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	894 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA ]
Biological Process:	leucyl-tRNA aminoacylation [IDA] Group I intron splicing [IMP]
Molecular Function:	leucine-tRNA ligase activity [IDA] mRNA binding [IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLSRPSSRFL STKRGPGPAV KKLIAIGEKW KQKTTRGLPK QDTLNSGSKY ILCQFPYPSG  60
   61 ALHIGHLRVY VISDSLNRFY KQKGYNVIHP MGWDAFGLPA ENAAIERSIN PAIWTRDNIA 120
  121 KMKQQMQSML ANFDWDREIT TCDPEYYKFT QWIFLKLFEN GLAYRKEAEI NWDPVDMTVL 180
  181 ANEQVDAQGR SWRSGAIVEK KQLKQWFLGI TKFAPKLKKH LNQLKDWPSN VKQMQKNWIG 240
  241 ESVGAELVFK VADPKFENLI VFTTRPETLF AVQYVALALD HPIVQKYCEE MPDLKEFIQK 300
  301 SDQLPNDTKE GFQLPNIKAV NPLTKEEVPI FAAPYVVSSY GSAPSAVMGC PGHDNRDFEF 360
  361 WQTNCPGEHI KTCIAPFFDD ASKVTEQERQ RIIDTVPFTS TDGVLTKECG EHSGVLTVVA 420
  421 RKSIMGMLNS EGLSKSVVRY KIRDWLISRQ RYWGTPIPII HCDNCGPVPV PESDLPVKLP 480
  481 ELEGLDTKGN PLSTIDEFVN VACPSCGSPA KRETDTMDTF IDSSWYYFRF LDPKNTSKPF 540
  541 DREIASKNMP VDIYIGGVEH AILHLLYSRF IAKFLGSINA WSDPAGIFEP FKKLVTQGMV 600
  601 QGKTYVDPDS GKFLKPDELT FVNDSPDGNT VIIKSNGKVP VVSYEKMSKS KYNGADPNEC 660
  661 ILRHGPDATR AHILFQSPIA DALNWDESKI VGIERWLQKV LHLTKNILSL EKDLAISKDY 720
  721 KTPTDLNDAE VKFHNDFQRF LKSITESFEV NLSLNTVISD YMKLTNILES ALKKGEVRNE 780
  781 MIVQNLQKLV TVIYPAVPSI SEEAAEMINS QMEWNQYRWP EVERTTESKF KKFQIVVNGR 840
  841 VKFMYTADKN FLKLGRDAVI ETLMNLPEGR MYLMNKKIKK FVMKFNVISF LFHK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		BI4 CBP6 MRS1 MRS2 MRS3 MRS4 MSS1 MSS116 MSS18 MTF2 NAM2 PET54 TIM10
View Details	Krogan NJ, et al. (2006)		NAM2 PAR32
View Details	Qiu et al. (2008)		BI3 BI4 MSE1 MSK1 MSM1 NAM2 PET54

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	NAM2	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..13] [707..739] [756..789] [823..894]	PSI-BLAST	290.228787	Isoleucyl-tRNA synthetase (IleRS)
2	View Details	[14..49] [61..89] [122..135] [597..706] [740..755] [790..822]	PSI-BLAST	290.228787	Isoleucyl-tRNA synthetase (IleRS)
3	View Details	[50..60] [90..121] [136..167] [411..596]	PSI-BLAST	290.228787	Isoleucyl-tRNA synthetase (IleRS)
4	View Details	[168..410]	PSI-BLAST	290.228787	Isoleucyl-tRNA synthetase (IleRS)

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: NAM2

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: