Protein Overview: TOP3

General Information:
Name(s) found:	TOP3 / YLR234W [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|ISBYT3 DNA topoisomerase (EC 5.99.1.2) III - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6323263\|ref\|NP_013335.1\| DNA Topoisomerase III, conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially, involved in telomere stability and regulation of mitotic recombinatio [NCBI NR] gi\|609366\|gb\|AAB67406.1\| Top3p: DNA Topoisomerase III [Saccharomyces cerevisiae] [NCBI NR] gi\|173002\|gb\|AAA35161.1\| DNA topoisomerase [NCBI NR] gi\|136001\|sp\|P13099.1\|TOP3_YEAST RecName: Full=DNA topoisomerase 3; AltName: Full=DNA topoisomerase III [NCBI NR] DNA topoisomerase 3 OS=Saccharomyces cerevisiae GN=TOP3 PE=1 SV=1 [Swiss-Prot] TOP3 DNA topoisomerase III [mips] DNA Topoisomerase III, conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially, involved in telomere stability and regulation of mitotic recombination [SGD] TOP3, Chr XII from 609783-611753 [yeast_orfs2] Chr XII from 609783-611753 [yeastorf3.fasta] (P13099) DNA topoisomerase III (EC 5.99.1.2) [4932.FASTACCERV.txt] TOP3 SGDID:S0004224, Chr XII from 609785-611755, Verified ORF [yeastflysequest.fasta] DNA Topoisomerase III [Saccharomyces cerevisiae]gi\|136001\|sp\|P13099\|TOP3_YEAST DNA topoisomerase IIIgi\|68485\|pir\|\|ISBYT3 DNA topoisomerase (EC 5.99.1.2) III - yeast (Saccharomyces cerevisiae)gi\|609366\|gb\|AAB67406.1\| Top3p: DNA Topoisomerase III [Saccha [nrdb_11152004_Chlamydomonas] TOP3 SGDID:S000004224, Chr XII from 609785-611755, Verified ORF, "DNA Topoisomerase III, conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially, involved in telomere stability [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Top3p: DNA Topoisomerase III [Saccharomyces cerevisiae] [nr-271106-contam.fasta] DNA Topoisomerase III, conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially, involved in telomere stability and regulation of mitotic recombination; Top3p [Saccharomyces cere [nr-271106-contam.fasta] DNA topoisomerase [nr-271106-contam.fasta] DNA topoisomerase 3 (DNA topoisomerase III) [nr-271106-contam.fasta] TOP3 SGDID:S000004224, Chr XII from 609785-611755, Verified ORF, "DNA Topoisomerase III, conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially, involved in telomere stability and regulation of mitotic recombination" [yeast-bovrabprots.fasta] [chemostatdb.fasta] DNA topoisomerase 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TOP3 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] TOP3 SGDID:S000004224, Chr XII from 609783-611753, Genome Release 64-1-1, Verified ORF, "DNA Topoisomerase III, conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially, involved in telomere stability and regulation of mitotic recombination" [yeast-030211-contam.fasta] TOP3 SGDID:S000004224, Chr XII from 609783-611753, Genome Release 64-2-1, Verified ORF, "DNA Topoisomerase III; conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially; DNA catenation/decatenation activity stimulated by RPA and Sgs1p-Top2p-Rmi1p; involved in telomere stability and regulation of mitotic recombination" [20141208_orf_trans.fasta] YLR234W SGDID:S000004224, Chr XII from 609783-611753, Genome Release 64-1-1, Verified ORF, "DNA Topoisomerase III, conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially, involved in telomere stability and regulation of mitotic recombination" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	656 amino acids
Gene Ontology:
Cellular Component:	nucleus [IC] RecQ helicase-Topo III complex [IDA][IPI]
Biological Process:	reciprocal meiotic recombination [IGI][IMP] telomere maintenance via telomerase [IMP] regulation of DNA recombination [IMP]
Molecular Function:	DNA topoisomerase type I activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKVLCVAEKN SIAKAVSQIL GGGRSTSRDS GYMYVKNYDF MFSGFPFARN GANCEVTMTS  60
   61 VAGHLTGIDF SHDSHGWGKC AIQELFDAPL NEIMNNNQKK IASNIKREAR NADYLMIWTD 120
  121 CDREGEYIGW EIWQEAKRGN RLIQNDQVYR AVFSHLERQH ILNAARNPSR LDMKSVHAVG 180
  181 TRIEIDLRAG VTFTRLLTET LRNKLRNQAT MTKDGAKHRG GNKNDSQVVS YGTCQFPTLG 240
  241 FVVDRFERIR NFVPEEFWYI QLVVENKDNG GTTTFQWDRG HLFDRLSVLT FYETCIETAG 300
  301 NVAQVVDLKS KPTTKYRPLP LTTVELQKNC ARYLRLNAKQ SLDAAEKLYQ KGFISYPRTE 360
  361 TDTFPHAMDL KSLVEKQAQL DQLAAGGRTA WASYAASLLQ PENTSNNNKF KFPRSGSHDD 420
  421 KAHPPIHPIV SLGPEANVSP VERRVYEYVA RHFLACCSED AKGQSMTLVL DWAVERFSAS 480
  481 GLVVLERNFL DVYPWARWET TKQLPRLEMN ALVDIAKAEM KAGTTAPPKP MTESELILLM 540
  541 DTNGIGTDAT IAEHIDKIQV RNYVRSEKVG KETYLQPTTL GVSLVHGFEA IGLEDSFAKP 600
  601 FQRREMEQDL KKICEGHASK TDVVKDIVEK YRKYWHKTNA CKNTLLQVYD RVKASM

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Gavin AC, et al. (2002)		CMR1 DNA2 HDA1 HDA2 HDA3 HSC82 KAP95 LCD1 MEC1 MGM101 MLH1 MPH1 MSH2 MSH3 MSH6 OLA1 PGK1 PMS1 PRO2 RAD16 RAD52 RFA1 RFA2 RFA3 RIM1 RNH1 RSC8 RVB2 SGS1 SHS1 TIF1, TIF2 TOP3 VAS1 VPS1 YHR122W
View Details	Qiu et al. (2008)		SGS1 TOP3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	TOP3	DUN1	2	Unpublished Fields Lab Data
View Screen	SGS1	TOP3	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	TOP3	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..37] [93..174]	PSI-BLAST	408.9897	DNA topoisomerase I, 67K N-terminal domain
2	View Details	[38..92] [175..253] [526..656]	PSI-BLAST	408.9897	DNA topoisomerase I, 67K N-terminal domain
3	View Details	[254..312] [464..483] [500..525]	PSI-BLAST	408.9897	DNA topoisomerase I, 67K N-terminal domain
4	View Details	[313..463] [484..499]	PSI-BLAST	408.9897	DNA topoisomerase I, 67K N-terminal domain

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
DNA topoisomerase activity	10.2798831210729	bayes_pls_golite062009
DNA topoisomerase type I activity	8.04585827786358	bayes_pls_golite062009
isomerase activity	5.61256457337998	bayes_pls_golite062009
DNA binding	5.20730175008144	bayes_pls_golite062009
nucleic acid binding	4.95671919332271	bayes_pls_golite062009
binding	4.3089127577748	bayes_pls_golite062009
catalytic activity	1.50449367975676	bayes_pls_golite062009
protein binding	0.377429709958808	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2