Protein Overview: SMC3

General Information:
Name(s) found:	SMC3 / YJL074C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|895899\|emb\|CAA61313.1\| hypothetical protein [Saccharomyces cerevisiae] [NCBI NR] gi\|6322387\|ref\|NP_012461.1\| Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase fa [NCBI NR] gi\|2570100\|emb\|CAA74655.1\| Smc3 [Saccharomyces cerevisiae] [NCBI NR] gi\|1352989\|sp\|P47037.1\|SMC3_YEAST RecName: Full=Structural maintenance of chromosomes protein 3; AltName: Full=DA-box protein SMC3 [NCBI NR] pir\|\|S56850 SMC1 protein homolog YJL074c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1008231\|emb\|CAA89366.1\| SMC3 [Saccharomyces cerevisiae] [NCBI NR] Structural maintenance of chromosomes protein 3 OS=Saccharomyces cerevisiae GN=SMC3 PE=1 SV=1 [Swiss-Prot] SMC3 Protein required for structural maintenance of chromosomes [mips] Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member [SGD] SMC3, Chr X from 298853-302545, reverse complement [yeast_orfs2] Chr X from 298853-302545, reverse complement [yeastorf3.fasta] Chr X from 302545-298853, reverse complement [yeastorf5.fasta] (P47037) Structural maintenance of chromosome 3 (DA-box protein SMC3) [4932.FASTACCERV.txt] SMC3 SGDID:S0003610, Chr X from 302765-299073, reverse complement, Verified ORF [yeastflysequest.fasta] Smc3p [Saccharomyces cerevisiae]gi\|895899\|emb\|CAA61313.1\| hypothetical protein [Saccharomyces cerevisiae]gi\|1008231\|emb\|CAA89366.1\| SMC3 [Saccharomyces cerevisiae]gi\|2570100\|emb\|CAA74655.1\| Smc3 [Saccharomyces cerevisiae]gi\|1352989\|sp\|P47037\|SMC3_YEAS [nrdb_11152004_Chlamydomonas] SMC3 SGDID:S000003610, Chr X from 302765-299073, reverse complement, Verified ORF, "Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meios [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] SMC3 SGDID:S000003610, Chr X from 302844-299152, reverse complement, Verified ORF, "Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meios [yeast-contam.fasta] SMC3 SGDID:S000003610, Chr X from 302843-299151, reverse complement, Verified ORF, "Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meios [yeast-200209-contam.fasta] SMC3 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member; Smc3p [Saccharo [nr-271106-contam.fasta] Structural maintenance of chromosome 3 (DA-box protein SMC3) [nr-271106-contam.fasta] hypothetical protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Smc3 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] SMC3 SGDID:S000003610, Chr X from 302843-299151, reverse complement, Verified ORF, "Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member" [yeast-bovrabprots.fasta] SMC3 SGDID:S000003610, Chr X from 302844-299152, reverse complement, Verified ORF, "Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member" [yeast-contam_custom.fasta] [chemostatdb.fasta] Structural maintenance of chromosomes protein 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SMC3 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SMC3 SGDID:S000003610, Chr X from 302849-299157, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member" [yeast-030211-contam.fasta] SMC3 SGDID:S000003610, Chr X from 302849-299157, Genome Release 64-2-1, reverse complement, Verified ORF, "Subunit of the multiprotein cohesin complex; required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member" [20141208_orf_trans.fasta] YJL074C SGDID:S000003610, Chr X from 302849-299157, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1230 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] nuclear cohesin complex [TAS]
Biological Process:	ascospore formation [IMP] mitotic sister chromatid cohesion [IMP][TAS] synaptonemal complex assembly [IMP]
Molecular Function:	ATPase activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MYIKRVIIKG FKTYRNETII DNFSPHQNVI IGSNGSGKSN FFAAIRFVLS DDYSNLKREE  60
   61 RQGLIHQGSG GSVMSASVEI VFHDPDHSMI LPSGVLSRGD DEVTIRRTVG LKKDDYQLND 120
  121 RNVTKGDIVR MLETAGFSMN NPYNIVPQGK IVALTNAKDK ERLQLLEDVV GAKSFEVKLK 180
  181 ASLKKMEETE QKKIQINKEM GELNSKLSEM EQERKELEKY NELERNRKIY QFTLYDRELN 240
  241 EVINQMERLD GDYNNTVYSS EQYIQELDKR EDMIDQVSKK LSSIEASLKI KNATDLQQAK 300
  301 LRESEISQKL TNVNVKIKDV QQQIESNEEQ RNLDSATLKE IKSIIEQRKQ KLSKILPRYQ 360
  361 ELTKEEAMYK LQLASLQQKQ RDLILKKGEY ARFKSKDERD TWIHSEIEEL KSSIQNLNEL 420
  421 ESQLQMDRTS LRKQYSAIDE EIEELIDSIN GPDTKGQLED FDSELIHLKQ KLSESLDTRK 480
  481 ELWRKEQKLQ TVLETLLSDV NQNQRNVNET MSRSLANGII NVKEITEKLK ISPESVFGTL 540
  541 GELIKVNDKY KTCAEVIGGN SLFHIVVDTE ETATLIMNEL YRMKGGRVTF IPLNRLSLDS 600
  601 DVKFPSNTTT QIQFTPLIKK IKYEPRFEKA VKHVFGKTIV VKDLGQGLKL AKKHKLNAIT 660
  661 LDGDRADKRG VLTGGYLDQH KRTRLESLKN LNESRSQHKK ILEELDFVRN ELNDIDTKID 720
  721 QVNGNIRKVS NDRESVLTNI EVYRTSLNTK KNEKLILEES LNAIILKLEK LNTNRTFAQE 780
  781 KLNTFENDLL QEFDSELSKE EKERLESLTK EISAAHNKLN ITSDALEGIT TTIDSLNAEL 840
  841 ESKLIPQEND LESKMSEVGD AFIFGLQDEL KELQLEKESV EKQHENAVLE LGTVQREIES 900
  901 LIAEETNNKK LLEKANNQQR LLLKKLDNFQ KSVEKTMIKK TTLVTRREEL QQRIREIGLL 960
  961 PEDALVNDFS DITSDQLLQR LNDMNTEISG LKNVNKRAFE NFKKFNERRK DLAERASELD1020
 1021 ESKDSIQDLI VKLKQQKVNA VDSTFQKVSE NFEAVFERLV PRGTAKLIIH RKNDNANDHD1080
 1081 ESIDVDMDAE SNESQNGKDS EIMYTGVSIS VSFNSKQNEQ LHVEQLSGGQ KTVCAIALIL1140
 1141 AIQMVDPASF YLFDEIDAAL DKQYRTAVAT LLKELSKNAQ FICTTFRTDM LQVADKFFRV1200
 1201 KYENKISTVI EVNREEAIGF IRGSNKFAEV

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		IRR1 MCD1 SMC1 SMC3
View Details	(MIPS) Mewes HW, et al. (2004)		IRR1 MCD1 REC8 SCC2 SMC1 SMC3
View Details	Krogan NJ, et al. (2006)		IRR1 MCD1 SMC1 SMC3
View Details	Gavin AC, et al. (2006)		SMC1 SMC3
View Details	Gavin AC, et al. (2002)		ACC1 CCT8 CLU1 CPA2 FDH1 GCN1 GCN20 GFA1 GUS1 HHF1, HHF2 ILV1 IRR1 KAP123 LYS12 MCD1 MRPL3 NOP12 PSA1 RPA135 RPB2 RPN10 RPN11 RPN12 RPN8 RPT3 RPT5 RPT6 RVB2 SAM1 SCC4 SEC27 SMC1 SMC3 TCB1 TIF1, TIF2 TSA1 TUB3 URA7 VAS1 YHB1 YOL098C
View Details	Ho Y, et al. (2002)		ASF1 CDC5 COP1 GFA1 GID8 GSY1 HTA2 IPP1 IRR1 IVY1 KAP95 MCD1 MDH1 NOP13 NUG1 PAA1 RAD53 RFC4 SMC1 SMC3 SPC72 SRP1 UBR1 URA7 UTP22 YGL081W
View Details	Qiu et al. (2008)		REC8 SMC3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	None Entered	#17 Mitotic Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	SMC3	Sundin BA, et al. (2004)
View Data	SMC3	Sundin BA, et al. (2004)
View Data	SMC3	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..50]	PSI-BLAST	2.69897	Myosin S1 fragment, N-terminal domain; Myosin S1, motor domain
2	View Details	[51..394]	PSI-BLAST	22.522879	Heavy meromyosin subfragment
3	View Details	[395..451]	deduced	N/A	No confident structure predictions are available.
4	View Details	[452..539] [563..596]	PSI-BLAST	91.154902	Smc hinge domain
5	View Details	[540..562] [597..600] [618..636]	PSI-BLAST	91.154902	Smc hinge domain
6	View Details	[601..617] [637..663]	PSI-BLAST	91.154902	Smc hinge domain
7	View Details	[664..730]	PSI-BLAST	9.045757	Tropomyosin
8	View Details	[731..992]	PSI-BLAST	9.045757	Tropomyosin
9	View Details	[993..1045]	deduced	N/A	No confident structure predictions are available.
10	View Details	[1046..1230]	PSI-BLAST	144.94696	Smc head domain

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Protein Overview: SMC3

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: