Protein Overview: NAS2

General Information:
Name(s) found:	NAS2 / YIL007C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|731776\|sp\|P40555.1\|PSMD9_YEAST RecName: Full=Probable 26S proteasome regulatory subunit p27; AltName: Full=Proteasome non-ATPase subunit 2 [NCBI NR] gi\|6322184\|ref\|NP_012259.1\| Protein with similarity to the p27 subunit of mammalian proteasome modulator; not essential; interacts with Rpn4p; Nas2p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S48450 hypothetical protein YIL007c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|558397\|emb\|CAA86244.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|45270478\|gb\|AAS56620.1\| YIL007C [Saccharomyces cerevisiae] [NCBI NR] Probable 26S proteasome regulatory subunit p27 OS=Saccharomyces cerevisiae GN=NAS2 PE=1 SV=1 [Swiss-Prot] (P40555) Probable 26S proteasome regulatory subunit p27 (Proteasome non-ATPase subunit 2) [Swiss-Prot] NAS2 Ubiquitin-dependent protein catabolism [mips] Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p [SGD] YIL007C, Chr IX from 342991-343653, reverse complement [yeast_orfs2] Chr IX from 342991-343653, reverse complement [yeastorf3.fasta] Chr IX from 343653-342991, reverse complement [yeastorf5.fasta] NAS2 SGDID:S0001269, Chr IX from 343653-342991, reverse complement, Verified ORF [yeastflysequest.fasta] Nas2p [Saccharomyces cerevisiae]gi\|558397\|emb\|CAA86244.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|626358\|pir\|\|S48450 hypothetical protein YIL007c - yeast (Saccharomyces cerevisiae)gi\|45270478\|gb\|AAS56620.1\| YIL007C [Saccharomyces cerevisia [nrdb_11152004_Chlamydomonas] NAS2 SGDID:S000001269, Chr IX from 343653-342991, reverse complement, Verified ORF, "Protein with similarity to the p27 subunit of mammalian proteasome modulator; not essential; interacts with Rpn4p" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Probable 26S proteasome regulatory subunit p27 (Proteasome non-ATPase subunit 2) [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YIL007C [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Protein with similarity to the p27 subunit of mammalian proteasome modulator; not essential; interacts with Rpn4p; Nas2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] NAS2 SGDID:S000001269, Chr IX from 343653-342991, reverse complement, Verified ORF, "Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator [SGD_S-cerevisiae_na_01-05-2010_reversed.fasta] [chemostatdb.fasta] NAS2 SGDID:S000001269, Chr IX from 343653-342991, reverse complement, Verified ORF, "Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p" [SGDyeast.20101221.fasta] Probable 26S proteasome regulatory subunit p27 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NAS2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] NAS2 SGDID:S000001269, Chr IX from 343656-342994, Genome Release 64-1-1, reverse complement, Verified ORF, "Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p" [yeast-030211-contam.fasta] NAS2 SGDID:S000001269, Chr IX from 343656-342994, Genome Release 64-2-1, reverse complement, Verified ORF, "Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress" [20141208_orf_trans.fasta] YIL007C SGDID:S000001269, Chr IX from 343656-342994, Genome Release 64-1-1, reverse complement, Verified ORF, "Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	220 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA]
Biological Process:	ubiquitin-dependent protein catabolic process [ISS]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MEEEELSKLL ANVKIDPSLT SRISQIDSFK LSELMVLKTD IETQLEAYFS VLEQQGIGMD  60
   61 SALVTPDGYP RSDVDVLQVT MIRKNVNMLK NDLNHLLQRS HVLLNQHFDN MNVKSNQDAR 120
  121 RNNDDQAIQY TIPFAFISEV VPGSPSDKAD IKVDDKLISI GNVHAANHSK LQNIQMVVMK 180
  181 NEDRPLPVLL LREGQILKTS LTPSRNWNGR GLLGCRIQEL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		NAS2 NTR2 YCL047C
View Details	Ho Y, et al. (2002)		ACO1 NAS2

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	NAS2	CCZ1	2	Unpublished Fields Lab Data
View Screen	NAS2	ESL2	2	Unpublished Fields Lab Data
View Screen	NAS2	YCL073C	2	Unpublished Fields Lab Data
View Screen	NAS2	RNP1	2	Unpublished Fields Lab Data
View Screen	NAS2	RPN4	2	Drees BL, et al. (2001)
View Screen	NAS2	RPT5	2	Unpublished Fields Lab Data
View Screen	NAS2	RPT4	2	Unpublished Fields Lab Data
View Screen	NAS2	RPH1	2	Unpublished Fields Lab Data
View Screen	NAS2	UME1	2	Unpublished Fields Lab Data
View Screen	RHO4	NAS2	2	Drees BL, et al. (2001)
View Screen	NAS2	DED81	2	Unpublished Fields Lab Data
View Screen	RHO1	NAS2	2	Drees BL, et al. (2001)
View Screen	PRE7	NAS2	2	Unpublished Fields Lab Data
View Screen	NAS2	PFS1	2	Uetz P, et al. (2000)
View Screen	NAS2	IKI1	2	Unpublished Fields Lab Data
View Screen	NAS2	MMS4	2	Unpublished Fields Lab Data
View Screen	NAS2	SAC1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	NAS2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..220]	PSI-BLAST	42.0	Protease Do (DegP, HtrA), C-terminal domains; Protease Do (DegP, HtrA), catalytic domain

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
peptidase activity	4.00482245922158	bayes_pls_golite062009
peptidase activity, acting on L-amino acid peptides	3.80641317919152	bayes_pls_golite062009
endopeptidase activity	3.69721977392816	bayes_pls_golite062009
protein binding	3.40921081688513	bayes_pls_golite062009
binding	3.2595117613307	bayes_pls_golite062009
hydrolase activity	2.69939003844937	bayes_pls_golite062009
transcription regulator activity	2.16834620509051	bayes_pls_golite062009
transcription activator activity	1.37255187012662	bayes_pls_golite062009
transporter activity	0.666562078558936	bayes_pls_golite062009
transmembrane transporter activity	0.510205657363692	bayes_pls_golite062009
enzyme binding	0.46071739042312	bayes_pls_golite062009
ubiquitin-protein ligase activity	0.342817187733892	bayes_pls_golite062009
small conjugating protein ligase activity	0.299572882681276	bayes_pls_golite062009
structural molecule activity	0.153277808949873	bayes_pls_golite062009
substrate-specific transporter activity	0.141613198202921	bayes_pls_golite062009
PDZ domain binding	0.0604467745298803	bayes_pls_golite062009
transcription factor binding	0.0283953080227368	bayes_pls_golite062009
cytoskeletal protein binding	0.0187659836234897	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2