Protein Overview: IPI1

General Information:
Name(s) found:	IPI1 / YHR085W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|731682\|sp\|P38803.1\|IPI1_YEAST RecName: Full=Pre-rRNA-processing protein IPI1; AltName: Full=Involved in processing IST2 protein 1 [NCBI NR] gi\|6321877\|ref\|NP_011953.1\| Essential component of the Rix1 complex (with Rix1p and Ipi3p) that is required for processing of ITS2 sequences from 35S pre-rRNA; Rix1 complex associates with Mdn1p in pre-60S ribosomal particles; Ipi1p [Saccharomyces cerevis [NCBI NR] pir\|\|S46805 hypothetical protein YHR085w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|500826\|gb\|AAB68883.1\| Yhr085wp [Saccharomyces cerevisiae] [NCBI NR] Uncharacterized protein YHR085W - Saccharomyces cerevisiae (Baker's yeast) [Swiss-Prot] Pre-rRNA-processing protein IPI1 OS=Saccharomyces cerevisiae GN=IPI1 PE=1 SV=1 [Swiss-Prot] IPI1 Essential protein, may be involved in rRNA processing [mips] Essential component of the Rix1 complex (with Rix1p and Ipi3p) that is required for processing of ITS2 sequences from 35S pre-rRNA; Rix1 complex associates with Mdn1p in pre-60S ribosomal particles [SGD] YHR085W, Chr VIII from 276765-277769 [yeast_orfs2] Chr VIII from 276765-277769 [yeastorf3.fasta] (P38803) Hypothetical 37.9 kDa protein in STE12-NAM8 intergenic region [4932.FASTACCERV.txt] IPI1 SGDID:S0001127, Chr VIII from 276765-277769, Uncharacterized ORF [yeastflysequest.fasta] IPI1 SGDID:S0001127, Chr VIII from 276765-277769, Verified ORF [10112005cervSGD.fasta] Ipi1p [Saccharomyces cerevisiae]gi\|731682\|sp\|P38803\|YHO5_YEAST Hypothetical 37.9 kDa protein in STE12-NAM8 intergenic regiongi\|626638\|pir\|\|S46805 hypothetical protein YHR085w - yeast (Saccharomyces cerevisiae)gi\|500826\|gb\|AAB68883.1\| Yhr085wp [Saccharo [nrdb_11152004_Chlamydomonas] IPI1 SGDID:S000001127, Chr VIII from 276766-277770, Verified ORF, "Protein of unknown function, essential for viability, may be involved in rRNA processing" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] IPI1 SGDID:S000001127, Chr VIII from 276766-277770, Verified ORF, "Essential component of the Rix1 complex (with Rix1p and Ipi3p) that is required for processing of ITS2 sequences from 35S pre-rRNA; Rix1 complex associates with Mdn1p in pre-60S ribosomal [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Protein of unknown function, essential for viability, may be involved in rRNA processing; Ipi1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Uncharacterized protein YHR085W [nr-271106-contam.fasta] Yhr085wp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] IPI1 SGDID:S000001127, Chr VIII from 276766-277770, Verified ORF, "Essential component of the Rix1 complex (with Rix1p and Ipi3p) that is required for processing of ITS2 sequences from 35S pre-rRNA; Rix1 complex associates with Mdn1p in pre-60S ribosomal particles" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Pre-rRNA-processing protein IPI1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=IPI1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] IPI1 SGDID:S000001127, Chr VIII from 276764-277768, Genome Release 64-1-1, Verified ORF, "Essential component of the Rix1 complex (with Rix1p and Ipi3p) that is required for processing of ITS2 sequences from 35S pre-rRNA; Rix1 complex associates with Mdn1p in pre-60S ribosomal particles" [yeast-030211-contam.fasta] IPI1 SGDID:S000001127, Chr VIII from 276764-277768, Genome Release 64-2-1, Verified ORF, "Component of the Rix1 complex and possibly pre-replicative complexes; required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-replicative complex (pre-RC) formation and maintenance during DNA replication licensing; relocalizes to the cytosol in response to hypoxia; essential gene" [20141208_orf_trans.fasta] TR1034\|c0_g1_i1\|g.809 ORF TR1034\|c0_g1_i1\|g.809 TR1034\|c0_g1_i1\|m.809 type:complete len:335 (+) TR1034\|c0_g1_i1:99-1103(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YHR085W SGDID:S000001127, Chr VIII from 276764-277768, Genome Release 64-1-1, Verified ORF, "Essential component of the Rix1 complex (with Rix1p and Ipi3p) that is required for processing of ITS2 sequences from 35S pre-rRNA; Rix1 complex associates with Mdn1p in pre-60S ribosomal particles" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_8193\|g.5863 ORF asmbl_8193\|g.5863 asmbl_8193\|m.5863 type:complete len:335 (+) asmbl_8193:99-1103(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_8195\|g.4945 ORF ID=asmbl_8195\|g.4945 ID=asmbl_8195\|m.4945 type:complete len:335 (+) ID=asmbl_8195:99-1103(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	334 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA ] nuclear outer membrane [IMP] nucleoplasm [IDA]
Biological Process:	rRNA processing [IMP] ribosomal large subunit assembly [IMP]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTKSRKQKQK KQDFLRKKLK VGKPKEKARN ATDTSFVSKT ISIRNQHLDQ NPHDLTKRLT  60
   61 LLKHHNINVR KETLTTFQKS IPSIIKSRLM TPLLTQSIPL ICDESQQVRQ GLIDLVDEIG 120
  121 SHDAEILKLH CNIFVLYINM AMTHIVTQIQ ADSTKFLSHL LKYCGDEVVR KSWVKLLNGV 180
  181 FGVLGWGQVG KNDSASIVQT KKRNAKYVTI HLNALYTLVE YGCQDERARS DGDTAETTED 240
  241 SGTLRNPYLI PDYPQPFEHL KLFTRELKVQ DATSSGVNAT LLSLATQDID TRKAVFIEQF 300
  301 LPIVRKKIEV IIKEGGECGK SANKLKTLLA KIFD

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ARX1 IPI1 IPI3 MDN1 MRT4 NOG2 NOP7 NUG1 RIX1 RSA4 SDA1
View Details	Hazbun TR, et al. (2003)		IPI1 IPI3 RIX1
View Details	Krogan NJ, et al. (2006)		IPI1 IPI3 RIX1
View Details	Gavin AC, et al. (2006)		CIC1 EBP2 IPI1 RPL31B
View Details	Gavin AC, et al. (2002)		ARX1 BEM2 BMS1 BRX1 BUD20 CIC1 CLU1 CYR1 CYS4 DBP10 DBP9 DCP2 DRS1 EBP2 ERB1 FPR4 GCN1 HAS1 HDA1 HER1 HIS3 INP2 IPI1 IPI3 KRE33 LHP1 LOC1 LSG1 MAK21 MAK5 MDN1 MIS1 MRT4 NAP1 NIP1 NIP7 NMD3 NOC2 NOC3 NOG1 NOG2 NOP1 NOP12 NOP15 NOP16 NOP2 NOP4 NOP56 NOP7 NSA1 NSA2 NUG1 PRT1 PUF6 REI1 RIX1 RIX7 RLP24 RLP7 RPA135 RPA190 RPF1 RPF2 RPG1 RPP2B RPT6 RRP1 RRP12 RSA3 RSA4 RTR1 SDA1 SEC7 SKI2 SNU13 SPB1 SPB4 SSF1 TIF6 TUB3 URB1 UTP10 UTP20 UTP22 YGL036W YTM1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RIX1	No Comments	Hazbun TR, et al. (2003)
View Run	RIX1	No Comments	Hazbun TR, et al. (2003)
View Run	IPI1	No Comments	Hazbun TR, et al. (2003)
View Run	IPI3	No Comments	Hazbun TR, et al. (2003)
View Run	RIX1	No Comments	Hazbun TR, et al. (2003)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	IPI1	NFI1	2	Hazbun TR, et al. (2003)
View Screen	IPI1	RSC3	2	Hazbun TR, et al. (2003)

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	IPI1 and SPC42	Hazbun TR, et al. (2003)
View Data	IPI1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..230]	MSA	6.009962	View MSA. No confident structure predictions are available.
2	View Details	[231..334]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.94

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2