Protein Overview: UBP6

General Information:
Name(s) found:	UBP6 / YFR010W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|836765\|dbj\|BAA09249.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|14318532\|ref\|NP_116665.1\| Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; deletion causes hypersensitivity to cycloheximide and other toxic compounds; Ubp6p [NCBI NR] gi\|1174863\|sp\|P43593.1\|UBP6_YEAST RecName: Full=Ubiquitin carboxyl-terminal hydrolase 6; AltName: Full=Ubiquitin thioesterase 6; AltName: Full=Ubiquitin-specific-processing protease 6; AltName: Full=Deubiquitinating enzyme 6 [NCBI NR] pir\|\|S56265 hypothetical protein YFR010w - yeast (Saccharomyces cerevisiae) [NCBI NR] Ubiquitin carboxyl-terminal hydrolase 6 OS=Saccharomyces cerevisiae GN=UBP6 PE=1 SV=1 [Swiss-Prot] UBP6 Ubiquitin-specific protease [mips] Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to polyubiquitin elongation activity of Hul5p [SGD] UBP6, Chr VI from 165059-166558 [yeast_orfs2] Chr VI from 165059-166558 [yeastorf3.fasta] Chr VI from 165060-166559 [yeastorf5.fasta] (P43593) Ubiquitin carboxyl-terminal hydrolase 6 (EC 3.1.2.15) (Ubiquitin thiolesterase 6) (Ubiquitin-specific processing protease 6) (Deubiquitinating enzyme 6) [4932.FASTACCERV.txt] UBP6 SGDID:S0001906, Chr VI from 165060-166559, Verified ORF [yeastflysequest.fasta] Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; deletion causes hypersensitivity to cycloheximide and other toxic compounds [Saccharomyces cerevisiae]gi\|108473 [nrdb_11152004_Chlamydomonas] UBP6 SGDID:S000001906, Chr VI from 165060-166559, Verified ORF, "Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; deletion causes hypersensitivity to cycloheximi [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] UBP6 SGDID:S000001906, Chr VI from 165060-166559, Verified ORF, "Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to polyubiquitin elongation [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; deletion causes hypersensitivity to cycloheximide and other toxic compounds; Ubp6p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Ubiquitin carboxyl-terminal hydrolase 6 (Ubiquitin thioesterase 6) (Ubiquitin-specific-processing protease 6) (Deubiquitinating enzyme 6) [nr-271106-contam.fasta] UBP6 SGDID:S000001906, Chr VI from 165060-166559, Verified ORF, "Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to polyubiquitin elongation activity of Hul5p" [yeast-bovrabprots.fasta] UBP6 SGDID:S000001906, Chr VI from 165060-166559, Verified ORF, "Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; deletion causes hypersensitivity to cycloheximide and other toxic compounds" [yeast-contam_custom.fasta] [chemostatdb.fasta] Ubiquitin carboxyl-terminal hydrolase 6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=UBP6 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] UBP6 SGDID:S000001906, Chr VI from 165067-166566, Genome Release 64-1-1, Verified ORF, "Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance" [yeast-030211-contam.fasta] UBP6 SGDID:S000001906, Chr VI from 165067-166566, Genome Release 64-2-1, Verified ORF, "Ubiquitin-specific protease; situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; negatively regulates degradation of ubiquitinated proteins by the proteasome; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance" [20141208_orf_trans.fasta] TR3855\|c0_g1_i1\|g.2695 ORF TR3855\|c0_g1_i1\|g.2695 TR3855\|c0_g1_i1\|m.2695 type:complete len:500 (+) TR3855\|c0_g1_i1:99-1598(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR6480\|c0_g1_i1\|g.4678 ORF TR6480\|c0_g1_i1\|g.4678 TR6480\|c0_g1_i1\|m.4678 type:complete len:500 (+) TR6480\|c0_g1_i1:91-1590(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YFR010W SGDID:S000001906, Chr VI from 165067-166566, Genome Release 64-1-1, Verified ORF, "Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_9423\|g.6927 ORF asmbl_9423\|g.6927 asmbl_9423\|m.6927 type:complete len:500 (+) asmbl_9423:83-1582(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] asmbl_9372\|g.6807 ORF asmbl_9372\|g.6807 asmbl_9372\|m.6807 type:complete len:500 (+) asmbl_9372:83-1582(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_9523\|g.5811 ORF ID=asmbl_9523\|g.5811 ID=asmbl_9523\|m.5811 type:complete len:500 (+) ID=asmbl_9523:83-1582(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_9379\|g.5727 ORF ID=asmbl_9379\|g.5727 ID=asmbl_9379\|m.5727 type:complete len:500 (+) ID=asmbl_9379:83-1582(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	499 amino acids
Gene Ontology:
Cellular Component:	proteasome regulatory particle [IPI]
Biological Process:	protein deubiquitination [TAS]
Molecular Function:	ubiquitin-specific protease activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSGETFEFNI RHSGKVYPIT LSTDATSADL KSKAEELTQV PSARQKYMVK GGLSGEESIK  60
   61 IYPLIKPGST VMLLGTPDAN LISKPAKKNN FIEDLAPEQQ VQQFAQLPVG FKNMGNTCYL 120
  121 NATLQALYRV NDLRDMILNY NPSQGVSNSG AQDEEIHKQI VIEMKRCFEN LQNKSFKSVL 180
  181 PIVLLNTLRK CYPQFAERDS QGGFYKQQDA EELFTQLFHS MSIVFGDKFS EDFRIQFKTT 240
  241 IKDTANDNDI TVKENESDSK LQCHISGTTN FMRNGLLEGL NEKIEKRSDL TGANSIYSVE 300
  301 KKISRLPKFL TVQYVRFFWK RSTNKKSKIL RKVVFPFQLD VADMLTPEYA AEKVKVRDEL 360
  361 RKVEKEKNEK EREIKRRKFD PSSSENVMTP REQYETQVAL NESEKDQWLE EYKKHFPPNL 420
  421 EKGENPSCVY NLIGVITHQG ANSESGHYQA FIRDELDENK WYKFNDDKVS VVEKEKIESL 480
  481 AGGGESDSAL ILMYKGFGL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ECM29 GSG1 NAS6 OSH7 POL4 RPN1 RPN10 RPN11 RPN12 RPN13 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 SEC18 SEM1 SLX5 SPG5 SWA2 UBP6 URA3 VPS45
View Details	Krogan NJ, et al. (2006)		CSI1 CSN9 ERV46 FPR2 HUL5 IQG1 MOT1 MPT5 NAS6 NMD3 NPT1 PCI8 PEP1 PST1 PUS9 RPN1 RPN10 RPN11 RPN12 RPN13 RPN14 RPN2 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 RRI1 RRI2 SEC18 SEM1 SKP1 SPG5 SPO77 SQT1 SWA2 TCB2 TIP41 TOD6 TOR2 TSR4 UBP6 YEN1 YKL027W YMR258C YNL022C YPR003C YPT52
View Details	Gavin AC, et al. (2002)		BLM10 CDC6 ECM29 GFA1 MLH2 NAS6 PGK1 PRE1 PRE10 PRE2 PRE3 PRE4 PRE5 PRE6 PRE7 PRE8 PRE9 PUP3 RGR1 RPN10 RPN11 RPN12 RPN13 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 SCL1 UBP6 YFL006W
View Details	Qiu et al. (2008)		DOA4 RPN12 RPN13 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 UBP6

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	UBP6	CYS3	2	Unpublished Fields Lab Data
View Screen	UBP6	YIL168W	2	Unpublished Fields Lab Data
View Screen	UBP6	CSR2	2	Unpublished Fields Lab Data
View Screen	UBP6	BNA4	2	Unpublished Fields Lab Data
View Screen	UBP6	TSC13	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	UBP6	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..80]	PSI-BLAST	8.39794	Ubiquitin
2	View Details	[81..155]	Pfam	14.031517	No description for PF00442 was found. No confident structure predictions are available.
3	View Details	[156..425]	MSA	6.241996	View MSA. No confident structure predictions are available.
4	View Details	[426..499]	Pfam	29.619789	Ubiquitin carboxyl-terminal hydrolase No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
small conjugating protein ligase activity	3.22676362920771	bayes_pls_golite062009
hydrolase activity	2.77871378503369	bayes_pls_golite062009
ubiquitin-protein ligase activity	2.46621820231139	bayes_pls_golite062009
acid-amino acid ligase activity	2.24025972049145	bayes_pls_golite062009
binding	2.19381585790185	bayes_pls_golite062009
protein tag	1.93603588492737	bayes_pls_golite062009
protein binding	1.84908810286528	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	1.54640383227873	bayes_pls_golite062009
SUMO ligase activity	0.58905960362002	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	0.536856055841234	bayes_pls_golite062009
kinase activity	0.506057697290914	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.410719674374641	bayes_pls_golite062009
nucleic acid binding	0.383121378043805	bayes_pls_golite062009
protein kinase activity	0.325253662829604	bayes_pls_golite062009
transferase activity	0.236208643697075	bayes_pls_golite062009
DNA binding	0.0840213088061073	bayes_pls_golite062009
transcription regulator activity	0.0485636215870291	bayes_pls_golite062009

Term	Confidence	Notes
peptidase activity	7.38847948226412	bayes_pls_golite062009
peptidase activity, acting on L-amino acid peptides	7.21002260103248	bayes_pls_golite062009
endopeptidase activity	6.57191549077589	bayes_pls_golite062009
cysteine-type peptidase activity	6.54685807643041	bayes_pls_golite062009
small conjugating protein-specific protease activity	6.39247995905454	bayes_pls_golite062009
ubiquitin-specific protease activity	5.91394445088241	bayes_pls_golite062009
hydrolase activity	4.82615927403291	bayes_pls_golite062009
ubiquitin thiolesterase activity	4.1832081096766	bayes_pls_golite062009
thiolester hydrolase activity	2.96735553959103	bayes_pls_golite062009
SUMO-specific protease activity	2.65263825059319	bayes_pls_golite062009
cysteine-type endopeptidase activity	1.95802900199539	bayes_pls_golite062009
binding	1.3276268995308	bayes_pls_golite062009
catalytic activity	1.05972260874031	bayes_pls_golite062009
protein binding	0.854677025220204	bayes_pls_golite062009
NEDD8-specific protease activity	0.687958315289315	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	0.495951820083869	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.467924587720185	bayes_pls_golite062009
nucleic acid binding	0.439226369799415	bayes_pls_golite062009
calcium-dependent cysteine-type endopeptidase activity	0.196538841514154	bayes_pls_golite062009
DNA binding	0.168761518285256	bayes_pls_golite062009
transferase activity	0.0852393573521809	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2