Protein Overview: SPT15

General Information:
Name(s) found:	SPT15 / YER148W [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|A30366 transcription initiation factor IID - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|73535263\|pdb\|1RM1\|A Chain A, Structure Of A Yeast TfiiaTBPTATA-Box Dna Complex [NCBI NR] gi\|6320996\|ref\|NP_011075.1\| TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability; Spt15p [Saccharomyces cerevisiae] [NCBI NR] gi\|603388\|gb\|AAB64675.1\| Spt15p: TATA-box binding protein tfIId [Saccharomyces cerevisiae] [NCBI NR] gi\|4610\|emb\|CAA34751.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|226502\|prf\|\|1515352A transcription initiation factor TFIID [NCBI NR] gi\|172901\|gb\|AAA35147.1\| TATA-binding protein [NCBI NR] gi\|171132\|gb\|AAA34458.1\| TATA box factor [NCBI NR] gi\|135643\|sp\|P13393.3\|TBP_YEAST RecName: Full=TATA-box-binding protein; AltName: Full=TATA-box factor; AltName: Full=TATA-binding factor; AltName: Full=TATA sequence-binding protein; Short=TBP; AltName: Full=Transcription factor D; AltName: Full=Transcrip [NCBI NR] TATA-box-binding protein OS=Saccharomyces cerevisiae GN=SPT15 PE=1 SV=3 [Swiss-Prot] SPT15 The TATA-binding protein TBP [mips] TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability [SGD] SPT15, Chr V from 465298-466020 [yeast_orfs2] Chr V from 465298-466020 [yeastorf3.fasta] SPT15 SGDID:S0000950, Chr V from 465298-466020, Verified ORF [yeastflysequest.fasta] Spt15p [Saccharomyces cerevisiae]gi\|4610\|emb\|CAA34751.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|603388\|gb\|AAB64675.1\| Spt15p: TATA-box binding protein tfIId [Saccharomyces cerevisiae]gi\|83563\|pir\|\|A30366 transcription initiation factor II [nrdb_11152004_Chlamydomonas] SPT15 SGDID:S000000950, Chr V from 465298-466020, Verified ORF, "TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] TATA-box-binding protein (TATA-box factor) (TATA-binding factor) (TATA sequence-binding protein) (TBP) (Transcription factor D) (Transcription initiation factor TFIID TBP subunit) [nr-271106-contam.fasta] Spt15p: TATA-box binding protein tfIId [Saccharomyces cerevisiae] [nr-271106-contam.fasta] transcription initiation factor TFIID [nr-271106-contam.fasta] TATA-binding protein [nr-271106-contam.fasta] Chain A, Structure Of A Yeast TfiiaTBPTATA-Box Dna Complex [nr-271106-contam.fasta] TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability; Spt15p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] TATA box factor [nr-271106-contam.fasta] [chemostatdb.fasta] TATA-box-binding protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SPT15 PE=1 SV=3 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SPT15 SGDID:S000000950, Chr V from 465303-466025, Genome Release 64-1-1, Verified ORF, "TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability" [yeast-030211-contam.fasta] SPT15 SGDID:S000000950, Chr V from 465303-466025, Genome Release 64-2-1, Verified ORF, "TATA-binding protein; general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability" [20141208_orf_trans.fasta] TR446\|c0_g1_i1\|g.222 ORF TR446\|c0_g1_i1\|g.222 TR446\|c0_g1_i1\|m.222 type:complete len:241 (+) TR446\|c0_g1_i1:294-1016(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR1395\|c0_g1_i1\|g.950 ORF TR1395\|c0_g1_i1\|g.950 TR1395\|c0_g1_i1\|m.950 type:complete len:241 (+) TR1395\|c0_g1_i1:643-1365(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YER148W SGDID:S000000950, Chr V from 465303-466025, Genome Release 64-1-1, Verified ORF, "TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_7655\|g.5471 ORF asmbl_7655\|g.5471 asmbl_7655\|m.5471 type:complete len:241 (+) asmbl_7655:476-1198(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_7633\|g.4841 ORF ID=asmbl_7633\|g.4841 ID=asmbl_7633\|m.4841 type:complete len:241 (+) ID=asmbl_7633:476-1198(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_7334\|g.4473 ORF ID=asmbl_7334\|g.4473 ID=asmbl_7334\|m.4473 type:complete len:241 (+) ID=asmbl_7334:294-1016(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	240 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] RNA polymerase I transcription factor complex [TAS] transcription factor TFIID complex [IDA][TAS] transcription factor TFIIIB complex [IDA][TAS]
Biological Process:	transcription from RNA polymerase III promoter [IMP] transcription initiation from RNA polymerase III promoter [TAS] transcription from RNA polymerase I promoter [IMP][TAS] transcription from RNA polymerase II promoter [IMP] transcription initiation from RNA polymerase II promoter [TAS ]
Molecular Function:	DNA binding [IPI][TAS] RNA polymerase I transcription factor activity [IMP][TAS] protein homodimerization activity [IMP] general RNA polymerase II transcription factor activity [IMP][TAS] DNA bending activity [IDA] RNA polymerase III transcription factor activity [IMP][TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA PESEKDTSAT  60
   61 SGIVPTLQNI VATVTLGCRL DLKTVALHAR NAEYNPKRFA AVIMRIREPK TTALIFASGK 120
  121 MVVTGAKSED DSKLASRKYA RIIQKIGFAA KFTDFKIQNI VGSCDVKFPI RLEGLAFSHG 180
  181 TFSSYEPELF PGLIYRMVKP KIVLLIFVSG KIVLTGAKQR EEIYQAFEAI YPVLSEFRKM 240
  241

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		MOT1 SPT15 TAF1 TAF11 TAF12 TAF3 TAF4 TAF5 TAF6 TAF9 VPS1
View Details	(MIPS) Mewes HW, et al. (2004)		BDP1 BRF1 RRN11 RRN6 RRN7 SPT15
View Details	Krogan NJ, et al. (2006)		BRF1 BRL1 SPT15
View Details	Gavin AC, et al. (2006)		SPT15 TAF1 TAF11 TAF12 TAF3 TAF4 TAF9
View Details	Gavin AC, et al. (2002)		ADA2 ADH1 CLU1 ESC8 FUN19 GCN1 GCN5 HEM13 IOC3 ISW1 ITC1 KAP114 KAP123 MED4 MES1 MNN4 MOT1 MYO1 NGG1 NOP1 NPL6 PDC1 PFK2 PSK1 PTC1 PUF6 RPB2 RPC40 RPN9 RPO21 RPT5 RSC2 RSC4 RSC58 RSC6 RSC8 RVB2 SGF29 SGF73 SIN3 SIN4 SPT15 SPT20 SPT7 SPT8 TAF1 TAF10 TAF11 TAF12 TAF13 TAF14 TAF2 TAF3 TAF4 TAF5 TAF6 TAF7 TAF8 TAF9 TBF1 TEF4 VAS1 VPS1 YEF3
View Details	Qiu et al. (2008)		BDP1 BRF1 PZF1 ROX3 RRN11 RRN3 RRN5 RRN6 RRN7 SPT15 TAF1 TAF10 TAF11 TAF13 TAF14 TAF3 TAF5 TAF6 TAF9 TFB5 TFC4 TFC8 UAF30

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	IST3	SPT15	2	Uetz P, et al. (2000)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	SPT15	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..69] [159..240]	PSI-BLAST	1037.616428	TATA-box binding protein (TBP), C-terminal domain
2	View Details	[70..158]	PSI-BLAST	1037.616428	TATA-box binding protein (TBP), C-terminal domain

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
general RNA polymerase II transcription factor activity	10.1927670598446	bayes_pls_golite062009
RNA polymerase III transcription factor activity	8.4318102330583	bayes_pls_golite062009
transcription regulator activity	4.66773099672992	bayes_pls_golite062009
RNA polymerase II transcription factor activity	3.97397050058114	bayes_pls_golite062009
DNA binding	3.68758425895596	bayes_pls_golite062009
nucleic acid binding	3.4119217152884	bayes_pls_golite062009
binding	3.25203283623613	bayes_pls_golite062009
RNA polymerase I transcription factor activity	3.24777019705869	bayes_pls_golite062009
transcription factor activity	2.17821704710246	bayes_pls_golite062009
transcription factor binding	1.86204668268867	bayes_pls_golite062009
specific RNA polymerase II transcription factor activity	1.59579825271822	bayes_pls_golite062009
protein binding	0.938536348073543	bayes_pls_golite062009
transcription coactivator activity	0.312991469396539	bayes_pls_golite062009
transcription activator activity	0.199341831249925	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2