YRC Public Data Repository - Protein Structure Prediction

Protein:	MBHT_ECOLI
Organism:	Escherichia coli
Length:	372 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for MBHT_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|192283313, gi... - gi\|192289564\|ref\|YP_001990169.1\| hydrogenase (NiFe) small subunit HydA [Rhodopseudomonas palustris T... hupS - hupS hydrogenase small chain 1064173:1065285 forward MW:40275	446.0	[0..2]	[370..6]
gi\|90425256, gi\|... - gi\|90425256\|ref\|YP_533626.1\| hydrogenase (NiFe) small subunit (hydA) [Rhodopseudomonas palustris Bis...	443.0	[0..2]	[364..6]
gi\|77701269, gi\|... - gi\|77738801\|ref\|ZP_00807294.1\| Ni-Fe hydrogenase, small subunit:Twin-arginine translocation pathway ...	443.0	[0..2]	[372..6]
gi\|124260787, gi... - gi\|124268012\|ref\|YP_001022016.1\| hydrogenase (acceptor) [Methylibium petroleiphilum PM1], gi\|1242607... gi\|47573671 - gi\|47573671\|ref\|ZP_00243709.1\| COG1740: Ni,Fe-hydrogenase I small subunit [Rubrivivax gelatinosus PM...	440.0	[0..1]	[328..1]
MBHS_ALCEU - (P31892) Uptake hydrogenase small subunit precursor (EC 1.12.99.6) (Hydrogenlyase) (Membrane-bound h... MBHS_CUPNH - Uptake hydrogenase small subunit OS=Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier... MBHS_RALEU - (P31892) Uptake hydrogenase small subunit precursor (EC 1.12.99.6) (Hydrogenlyase) (Membrane-bound h...	438.0	[0..1]	[331..2]
gi\|158329111, gi... - gi\|158422222\|ref\|YP_001523514.1\| Ni-Fe hydrogenase small subunit protein [Azorhizobium caulinodans O... gi\|46392530 - gi\|46392530\|gb\|AAS91026.1\| HupS [Azorhizobium caulinodans]	438.0	[0..1]	[331..4]
gi\|126462907, gi... - gi\|83373097\|ref\|ZP_00917876.1\| Ni-Fe hydrogenase, small subunit:Twin-arginine translocation pathway ...	437.0	[0..1]	[372..4]
gi\|77388481, gi\|... - gi\|77464063\|ref\|YP_353567.1\| hydrogenase protein small subunit [Rhodobacter sphaeroides 2.4.1], gi\|7...	436.0	[0..1]	[372..4]
gi\|83368440, gi\|... - gi\|83368440\|ref\|ZP_00913303.1\| Ni-Fe hydrogenase, small subunit:Twin-arginine translocation pathway ...	436.0	[0..1]	[370..4]
gi\|121604876, gi... - gi\|84712549\|ref\|ZP_01020429.1\| Ni-Fe hydrogenase, small subunit:Twin-arginine translocation pathway ...	435.0	[0..1]	[328..1]

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Predicted Domain #1

Region A:
Residues: [1-372]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTGDNTLIHS HGINRRDFMK LCAALAATMG LSSKAAAEMA ESVTNPQRPP VIWIGAQECT  60
   61 GCTESLLRAT HPTVENLVLE TISLEYHEVL SAAFGHQVEE NKHNALEKYK GQYVLVVDGS 120
  121 IPLKDNGIYC MVAGEPIVDH IRKAAEGAAA IIAIGSCSAW GGVAAAGVNP TGAVSLQEVL 180
  181 PGKTVINIPG CPPNPHNFLA TVAHIITYGK PPKLDDKNRP TFAYGRLIHE HCERRPHFDA 240
  241 GRFAKEFGDE GHREGWCLYH LGCKGPETYG NCSTLQFCDV GGVWPVAIGH PCYGCNEEGI 300
  301 GFHKGIHQLA NVENQTPRSQ KPDVNAKEGG NVSAGAIGLL GGVVGLVAGV SVMAVRELGR 360
  361 QQKKDNADSR GE

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	102.0
Match:	1h2aS
Description:	Nickel-iron hydrogenase, small subunit
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
oxidoreductase activity, acting on hydrogen as donor	2.20752608202439	bayes_pls_golite062009
ferredoxin hydrogenase activity	1.32333392056778	bayes_pls_golite062009
oxidoreductase activity, acting on hydrogen as donor, iron-sulfur protein as acceptor	1.32333392056778	bayes_pls_golite062009
NADH dehydrogenase activity	0.84569674518508	bayes_pls_golite062009
oxidoreductase activity, acting on NADH or NADPH	0.554599825830849	bayes_pls_golite062009
catalytic activity	0.513070432311783	bayes_pls_golite062009
NADH dehydrogenase (quinone) activity	0.463241887648122	bayes_pls_golite062009
oxidoreductase activity, acting on iron-sulfur proteins as donors	0.39909556472213	bayes_pls_golite062009
oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor	0.314635495446813	bayes_pls_golite062009
oxidoreductase activity	0.0918357876652069	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: