YRC Public Data Repository - Protein Structure Prediction

Protein:	GLNE_ECOLI
Organism:	Escherichia coli
Length:	946 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for GLNE_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|26249632, gi\|... - gi\|26249632\|ref\|NP_755672.1\| Glutamate-ammonia-ligase adenylyltransferase [Escherichia coli CFT073],...	891.0	[0..1]	[946..38]
gi\|110616488, gi... - gi\|110806940\|ref\|YP_690460.1\| bifunctional glutamine-synthetase adenylyltransferase/deadenyltransfer...	891.0	[0..1]	[946..30]
gi\|189358574, gi... - gi\|189358574\|gb\|EDU76993.1\| glutamate-ammonia ligase adenylyltransferase [Escherichia coli O157:H7 s... gi\|168747439, gi... - gi\|188017833\|gb\|EDU55955.1\| glutamate-ammonia ligase adenylyltransferase [Escherichia coli O157:H7 s... gi\|168767074, gi... - gi\|189363719\|gb\|EDU82138.1\| glutamate-ammonia ligase adenylyltransferase [Escherichia coli O157:H7 s... gi\|168760212, gi... - gi\|189369360\|gb\|EDU87776.1\| glutamate-ammonia ligase adenylyltransferase [Escherichia coli O157:H7 s... gi\|25348787, gi\|... - pir\|\|G85965 adenylylating enzyme for glutamine synthetase [imported] - Escherichia coli (strain O157... gi\|13363409, gi\|... - gi\|15833190\|ref\|NP_311963.1\| bifunctional glutamine-synthetase adenylyltransferase/deadenyltransfera... gi\|12517629, gi\|... - gi\|15803595\|ref\|NP_289628.1\| bifunctional glutamine-synthetase adenylyltransferase/deadenyltransfera... GLNE_ECO57 - Glutamate-ammonia-ligase adenylyltransferase OS=Escherichia coli O157:H7 GN=glnE PE=3 SV=1 gi\|254795004, gi... - gi\|254795004\|ref\|YP_003079841.1\| multifunctional deadenylyltransferase and adenylyltransferase for g... gi\|208818609, gi... - gi\|208818609\|ref\|ZP_03258929.1\| glutamate-ammonia ligase adenylyltransferase [Escherichia coli O157:... gi\|208812258, gi... - gi\|208812258\|ref\|ZP_03253587.1\| glutamate-ammonia ligase adenylyltransferase [Escherichia coli O157:... gi\|217319631, gi... - gi\|217327264\|ref\|ZP_03443347.1\| glutamate-ammonia ligase adenylyltransferase [Escherichia coli O157:... GLNE_ECO5E - Glutamate-ammonia-ligase adenylyltransferase OS=Escherichia coli O157:H7 (strain EC4115 / EHEC) GN=g... gi\|168781927, gi... - gi\|189000607\|gb\|EDU69593.1\| glutamate-ammonia ligase adenylyltransferase [Escherichia coli O157:H7 s... gi\|187770807, gi... - gi\|187770807\|gb\|EDU34651.1\| glutamate-ammonia ligase adenylyltransferase [Escherichia coli O157:H7 s... gi\|208805800, gi... - gi\|208805800\|ref\|ZP_03248137.1\| glutamate-ammonia ligase adenylyltransferase [Escherichia coli O157:... gi\|195937197 - gi\|195937197\|ref\|ZP_03082579.1\| bifunctional glutamine-synthetase adenylyltransferase/deadenyltransf...	890.0	[0..1]	[946..1]
gi\|188487013, gi... - gi\|75511263\|ref\|ZP_00733961.1\| COG1391: Glutamine synthetase adenylyltransferase [Escherichia coli 5...	888.0	[0..1]	[946..1]
gi\|75259986, gi\|... - gi\|75259986\|ref\|ZP_00731264.1\| COG1391: Glutamine synthetase adenylyltransferase [Escherichia coli E... gi\|194426367, gi... - gi\|75212314\|ref\|ZP_00712338.1\| COG1391: Glutamine synthetase adenylyltransferase [Escherichia coli B... GLNE_ECOSE - Glutamate-ammonia-ligase adenylyltransferase OS=Escherichia coli (strain SE11) GN=glnE PE=3 SV=1	888.0	[0..1]	[946..1]
GLNE_ECOHS - Glutamate-ammonia-ligase adenylyltransferase OS=Escherichia coli O9:H4 (strain HS) GN=glnE PE=3 SV=1	888.0	[0..1]	[946..1]
gi\|193067248, gi... - gi\|75239376\|ref\|ZP_00723347.1\| COG1391: Glutamine synthetase adenylyltransferase [Escherichia coli E...	888.0	[0..1]	[946..1]
GLNE_SHIB3 - Glutamate-ammonia-ligase adenylyltransferase OS=Shigella boydii serotype 18 (strain CDC 3083-94 / BS...	887.0	[0..1]	[946..1]
gi\|226899227, gi... - gi\|237706187\|ref\|ZP_04536668.1\| glutamate-ammonia-ligase adenylyltransferase [Escherichia sp. 3_2_53... gi\|91074055, gi\|... - gi\|91212481\|ref\|YP_542467.1\| glutamate-ammonia-ligase adenylyltransferase [Escherichia coli UTI89], ...	887.0	[0..1]	[946..38]
GLNE_SHIBS - Glutamate-ammonia-ligase adenylyltransferase OS=Shigella boydii serotype 4 (strain Sb227) GN=glnE PE...	886.0	[0..1]	[946..1]

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Predicted Domain #1

Region A:
Residues: [1-440]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKPLSSPLQQ YWQTVVERLP EPLAEESLSA QAKSVLTFSD FVQDSVIAHP EWLTELESQP  60
   61 PQADEWQHYA AWLQEALCNV SDEAGLMREL RLFRRRIMVR IAWAQTLALV TEESILQQLS 120
  121 YLAETLIVAA RDWLYDACCR EWGTPCNAQG EAQPLLILGM GKLGGGELNF SSDIDLIFAW 180
  181 PEHGCTQGGR RELDNAQFFT RMGQRLIKVL DQPTQDGFVY RVDMRLRPFG ESGPLVLSFA 240
  241 ALEDYYQEQG RDWERYAMVK ARIMGDSEGV YANELRAMLR PFVFRRYIDF SVIQSLRNMK 300
  301 GMIAREVRRR GLTDNIKLGA GGIREIEFIV QVFQLIRGGR EPSLQSRSLL PTLSAIAELH 360
  361 LLSENDAEQL RVAYLFLRRL ENLLQSINDE QTQTLPSDEL NRARLAWAMD FADWPQLTGA 420
  421 LTAHMTNVRR VFNELIGDDE

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	125.0
Match:	1v4aA
Description:	Structure of the N-terminal Domain of Escherichia coli Glutamine Synthetase adenylyltransferase
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
transferase activity, transferring phosphorus-containing groups	2.41308867449214	bayes_pls_golite062009
[glutamate-ammonia-ligase] adenylyltransferase activity	1.82264747359149	bayes_pls_golite062009
transferase activity	1.78252455260241	bayes_pls_golite062009
catalytic activity	1.122769147009	bayes_pls_golite062009
nucleotidyltransferase activity	0.78517703623017	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [441-518]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SETQEESLSE QWRELWQDAL QEDDTTPVLA HLSEDDRKQV LTLIADFRKE LDKRTIGPRG  60
   61 RQVLDHLMPH LLSDVCAR

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #3

Region A:
Residues: [519-946]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 EDAAVTLSRI TALLVGIVTR TTYLELLSEF PAALKHLISL CAASPMIASQ LARYPLLLDE  60
   61 LLDPNTLYQP TATDAYRDEL RQYLLRVPED DEEQQLEALR QFKQAQLLRI AAADIAGTLP 120
  121 VMKVSDHLTW LAEAMIDAVV QQAWVQMVAR YGKPNHLNER EGRGFAVVGY GKLGGWELGY 180
  181 SSDLDLIFLH DCPMDAMTDG EREIDGRQFY LRLAQRIMHL FSTRTSSGIL YEVDARLRPS 240
  241 GAAGMLVTSA EAFADYQKNE AWTWEHQALV RARVVYGDPQ LTAHFDAVRR EIMTLPREGK 300
  301 TLQTEVREMR EKMRAHLGNK HRDRFDIKAD EGGITDIEFI TQYLVLRYAH EKPKLTRWSD 360
  361 NVRILELLAQ NDIMEEQEAM ALTRAYTTLR DELHHLALQE LPGHVSEDCF TAERELVRAS 420
  421 WQKWLVEE

[Run NCBI BLAST on this sequence.]

Detection Method:	FFAS03
Confidence:	8.72
Match:	1v4aA
Description:	Structure of the N-terminal Domain of Escherichia coli Glutamine Synthetase adenylyltransferase
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
transferase activity, transferring phosphorus-containing groups	2.15416299307909	bayes_pls_golite062009
transferase activity	1.59622808836876	bayes_pls_golite062009
[glutamate-ammonia-ligase] adenylyltransferase activity	1.37668296387793	bayes_pls_golite062009
catalytic activity	1.08923954036615	bayes_pls_golite062009
nucleotidyltransferase activity	0.381583309516406	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions:

Predicted functions: