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View Structure Prediction Details

Protein: Brf-PA, Brf-PB
Organism: Drosophila melanogaster
Length: 662 amino acids
Reference: Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for Brf-PA, Brf-PB.

Description E-value Query
Range
Subject
Range
gi|109480055, gi... - gi|62651305|ref|XP_216801.3| PREDICTED: similar to transcription initiation factor IIIB [Rattus norv...
458.0 [0..3] [662..2]

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Predicted Domain #1
Region A:
Residues: [1-70]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSTGLKCRNC GSNEIEEDNA RGDRVCMNCG SVLEDSLIVS EVQFEEVGHG AAAIGQFVSA  60
   61 ESSGGATNYG 

[Run NCBI BLAST on this sequence.]

Detection Method: FFAS03
Confidence: 2.67
Match: 1rlyA
Description: RDC-derived models of the zinc ribbon domain of human general transcription TFIIB (zinc bound structures)
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
nucleic acid binding 2.22680674586367 bayes_pls_golite062009

Predicted Domain #2
Region A:
Residues: [71-283]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 YGKFQVGSGT ESREVTIKKA KKDITLLCQQ LQLSQHYADT ALNFFKMALG RHLTRGRKST  60
   61 HIYAACVYMT CRTEGTSHLL IDISDVQQIC SYELGRTYLK LSHALCINIP SLDPCLYIMR 120
  121 FANRLQLGAK THEVSMTALR IVQRMKKDCM HSGRRPTGLC GAALLIAARM HDFSRTMLDV 180
  181 IGVVKIHEST LRKRLSEFAE TPSGGLTLEE FMT

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 62.045757
Match: 1c9bA
Description: Transcription factor IIB (TFIIB), core domain
Matching Structure (courtesy of the PDB):

Predicted Domain #3
Region A:
Residues: [284-417]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 VDLEREQDPP SFKAARKKDR ERIKDMGEHE LTELQKEIDA HLEKDLGKYS NSVYRQLTKG  60
   61 KGLSPLSSPS TPNSSSEKDI ELEESRQFIE QSNAEVIKEL IAKNEDVKKS EPGGLVAGIE 120
  121 GLRPDIEAIC RVTQ

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #4
Region A:
Residues: [418-488]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SDLEDVEKAK QPQEQELITD DLNDDELDQY VLTEEESVAK LEMWKNLNAE YLQEQKERDE  60
   61 RLAKEREEGK P

[Run NCBI BLAST on this sequence.]

Detection Method: FFAS03
Confidence: 2.39
Match: 1ngmB
Description: TBP-binding domain of the transcription factor IIIb Brf1 subunit
Matching Structure (courtesy of the PDB):

Predicted Domain #5
Region A:
Residues: [489-662]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 ERKKRKPRKK VIGPSSTAGE AIEKMLQEKK ISSKINYEIL KTLTDGMGGL TDDSPTTSAD  60
   61 TKPSTLEELK HQPVIVEEGP VASKSRGNRA AYDLPGPSRK RPKLEVGLPV SKAADVEQAE 120
  121 TKPAVVVEAD DLDEDADDPD VEPEAEPEAT LQDMLNTGGD DDEFGYGFDE EEEY

[Run NCBI BLAST on this sequence.]

Detection Method: MSA

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.


YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle