YRC Public Data Repository - Protein Structure Prediction

Protein:	THI6
Organism:	Saccharomyces cerevisiae
Length:	540 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for THI6.

Description	E-value	Query Range	Subject Range
THI6 - Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazo... gi\|190407749 - gi\|190407749\|gb\|EDV11014.1\| hypothetical protein SCRG_02285 [Saccharomyces cerevisiae RM11-1a] THI6_YEAST - Thiamine biosynthetic bifunctional enzyme OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) G... gi\|151942587 - gi\|151942587\|gb\|EDN60933.1\| hydroxyethylthiazole kinase [Saccharomyces cerevisiae YJM789]	0.0	[1..540]	[1..540]
THI6_SCHPO - Probable thiamine biosynthetic bifunctional enzyme OS=Schizosaccharomyces pombe (strain 972 / ATCC 2... thi4 - thiamine-phosphate dipyrophosphorylase /hydroxyethylthiazole kinase	1.0E-97	[5..537]	[2..513]
THIED_COREF - Thiamine biosynthesis multifunctional protein ThiED OS=Corynebacterium efficiens (strain DSM 44549 /... THED_COREF, THIE... - Thiamine biosynthesis multifunctional protein ThiED OS=Corynebacterium efficiens GN=thiED PE=3 SV=1,...	5.0E-97	[9..516]	[3..482]
gi\|85081198 - gi\|85081198\|ref\|XP_956678.1\| hypothetical protein [Neurospora crassa OR74A] gi\|28917751, gi\|... - gi\|32403276\|ref\|XP_322251.1\| hypothetical protein [Neurospora crassa], gi\|28917751\|gb\|EAA27442.1\| hy...	2.0E-88	[15..537]	[224..729]
gi\|28211403, gi\|... - gi\|28211403\|ref\|NP_782347.1\| hydroxymethylpyrimidine kinase [Clostridium tetani E88], gi\|28203844\|gb...	3.0E-70	[232..533]	[1..296]
gi\|18145000, gi\|... - gi\|18310320\|ref\|NP_562254.1\| phosphomethylpyrimidine kinase [Clostridium perfringens str. 13], gi\|18...	3.0E-64	[242..515]	[8..260]
gi\|27468610, gi\|... - gi\|27468610\|ref\|NP_765247.1\| phosphomethylpyrimidine kinase [Staphylococcus epidermidis ATCC 12228],... gi\|57638290, gi\|... - gi\|57867632\|ref\|YP_189265.1\| phosphomethylpyrimidine kinase [Staphylococcus epidermidis RP62A], gi\|5...	7.0E-64	[238..526]	[4..273]
gi\|22776153, gi\|... - gi\|23097929\|ref\|NP_691395.1\| phosphomethylpyrimidine kinase [Oceanobacillus iheyensis HTE831], gi\|22...	7.0E-64	[237..514]	[3..258]
gi\|25326092 - pir\|\|F97951 phosphomethylpyrimidine kinase (EC 2.7.4.7) [imported] - Streptococcus pneumoniae (strai... gi\|15458222, gi\|... - gi\|15902682\|ref\|NP_358232.1\| phosphomethylpyrimidine kinase [Streptococcus pneumoniae R6], gi\|154582... gi\|237821850 - gi\|237821850\|ref\|ZP_04597695.1\| phosphomethylpyrimidine kinase [Streptococcus pneumoniae CCRI 1974M2... gi\|237651010 - gi\|237651010\|ref\|ZP_04525262.1\| phosphomethylpyrimidine kinase [Streptococcus pneumoniae CCRI 1974] gi\|116077129, gi... - gi\|116516553\|ref\|YP_816128.1\| phosphomethylpyrimidine kinase [Streptococcus pneumoniae D39], gi\|1160...	3.0E-63	[239..514]	[5..258]
gi\|15900623, gi\|... - gi\|66878133\|ref\|ZP_00403632.1\| COG0351: Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase [Stre... gi\|25326080 - pir\|\|B95084 phosphomethylpyrimidine kinase [imported] - Streptococcus pneumoniae (strain TIGR4) gi\|225728252, gi... - gi\|225860704\|ref\|YP_002742213.1\| phosphomethylpyrimidine kinase [Streptococcus pneumoniae Taiwan19F-...	3.0E-63	[239..514]	[5..258]

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Predicted Domain #1

Region A:
Residues: [1-233]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVFTKEEVDY SLYLVTDSTM LPPGTTLCSQ VEAGLKNGVT LVQIREKDIE TKNFVAEALE  60
   61 VQKICKKYNV PLIINDRIDV AMAIDADGVH VGQDDMPIPM VRKLLGPSKI LGWSVGKPSE 120
  121 VETLAKWGPD MVDYIGVGTL FPTSTKKNPK KSPMGPQGAI AILDALEEFK ATWCRTVGIG 180
  181 GLHPDNIQRV LCQCVASNGK RSLDGISLVS DIMAAPDACA ATKRLRGLLD ATR

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	241.228787
Match:	1g4tA_
Description:	Thiamin phosphate synthase
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
thiamin-phosphate diphosphorylase activity	5.90476603396884	bayes_pls_golite062009
phosphomethylpyrimidine kinase activity	1.96653650813022	bayes_pls_golite062009
catalytic activity	1.94308403509017	bayes_pls_golite062009
transferase activity	1.66762065968825	bayes_pls_golite062009
transferase activity, transferring alkyl or aryl (other than methyl) groups	0.73172690404654	bayes_pls_golite062009
phosphotransferase activity, phosphate group as acceptor	0.429889467826441	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [234-540]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 YQFVECELNN TFPTTTSIQN VISQVSNNRP LVQHITNKVH QNFGANVTLA LGSSPIMSEI  60
   61 ESEVSELARI PNASLLLNTG SVAPIEMLKA AINAYNEVNR PITFDPVGYS ATETRLCLNN 120
  121 TLLTYGQFAC IKGNCSEILS LAKLNNHKMK GVDSSSGKTN IDTLVRATQI VAFQYRTVAV 180
  181 CTGEFDCVAD GTFGGEYKLS SGTEGITAED LPCVIIEDGP IPIMGDITAS GCSLGSTIAS 240
  241 FIGGLDSTGK LFDAVVGAVL LYKSAGKLAS TRCQGSGSFH VELIDALYQL FHENKPEKWS 300
  301 ASLKKFK

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	266.616428
Match:	1c3qA_
Description:	Hydroxyethylthiazole kinase (THZ kinase, ThiK)
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
phosphomethylpyrimidine kinase activity	3.972577109569	bayes_pls_golite062009
phosphotransferase activity, phosphate group as acceptor	2.29466565429735	bayes_pls_golite062009
kinase activity	2.28531824242987	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	2.27980720248925	bayes_pls_golite062009
transferase activity	2.15883296431019	bayes_pls_golite062009
catalytic activity	1.74474587246986	bayes_pls_golite062009
hydroxyethylthiazole kinase activity	1.28568044601242	bayes_pls_golite062009
thiamin-phosphate diphosphorylase activity	1.27602595951972	bayes_pls_golite062009
carbohydrate kinase activity	1.12876288824319	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	0.673555832721854	bayes_pls_golite062009
pyridoxal kinase activity	0.217863391052366	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions:

Predicted functions: