YRC Public Data Repository - Protein Structure Prediction

Protein:	NIT2
Organism:	Saccharomyces cerevisiae
Length:	307 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for NIT2.

Description	E-value	Query Range	Subject Range
NIT2_YEAST - Probable hydrolase NIT2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NIT2 PE=1 SV=1 NIT2 - Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from ...	1.0E-78	[1..307]	[1..307]
NIT2 - nitrilase family, member 2	2.0E-70	[1..306]	[1..269]
NIT2_MOUSE - Omega-amidase NIT2 OS=Mus musculus GN=Nit2 PE=1 SV=1	4.0E-70	[5..306]	[3..269]
gi\|225726488, gi... - gi\|225861184\|ref\|YP_002742693.1\| N-carbamoylputrescine amidase [Streptococcus pneumoniae Taiwan19F-1... gi\|225858720, gi... - gi\|225858720\|ref\|YP_002740230.1\| N-carbamoylputrescine amidase [Streptococcus pneumoniae 70585], gi\|... gi\|225725231, gi... - gi\|225856586\|ref\|YP_002738097.1\| N-carbamoylputrescine amidase [Streptococcus pneumoniae P1031], gi\|... gi\|225854429, gi... - gi\|225854429\|ref\|YP_002735941.1\| N-carbamoylputrescine amidase [Streptococcus pneumoniae JJA], gi\|22... gi\|147764916, gi... - gi\|149010435\|ref\|ZP_01831806.1\| carbon-nitrogen hydrolase family protein [Streptococcus pneumoniae S... gi\|147930884, gi... - gi\|149019509\|ref\|ZP_01834828.1\| carbon-nitrogen hydrolase family protein [Streptococcus pneumoniae S... gi\|147759404, gi... - gi\|149002467\|ref\|ZP_01827401.1\| carbon-nitrogen hydrolase family protein [Streptococcus pneumoniae S... gi\|147762180, gi... - gi\|149006403\|ref\|ZP_01830115.1\| carbon-nitrogen hydrolase family protein [Streptococcus pneumoniae S... gi\|14972396, gi\|... - gi\|66878278\|ref\|ZP_00403767.1\| COG0388: Predicted amidohydrolase [Streptococcus pneumoniae TIGR4], g... gi\|194398116, gi... - gi\|194398116\|ref\|YP_002037559.1\| N-carbamoylputrescine amidase [Streptococcus pneumoniae G54], gi\|19... gi\|25320753 - pir\|\|E95106 carbon-nitrogen hydrolase family protein [imported] - Streptococcus pneumoniae (strain T... gi\|168485872, gi... - gi\|183571035\|gb\|EDT91563.1\| N-carbamoylputrescine amidase [Streptococcus pneumoniae CDC1087-00], gi\|... gi\|168491010, gi... - gi\|183574681\|gb\|EDT95209.1\| N-carbamoylputrescine amidase [Streptococcus pneumoniae CDC0288-04], gi\|... gi\|183578517, gi... - gi\|183578517\|gb\|EDT99045.1\| N-carbamoylputrescine amidase [Streptococcus pneumoniae MLV-016], gi\|168... gi\|169833217, gi... - gi\|169833217\|ref\|YP_001694375.1\| N-carbamoylputrescine amidase [Streptococcus pneumoniae Hungary19A-... gi\|182683869, gi... - gi\|182683869\|ref\|YP_001835616.1\| carbon-nitrogen hydrolase family protein [Streptococcus pneumoniae ... gi\|147928397, gi... - gi\|148992945\|ref\|ZP_01822564.1\| carbon-nitrogen hydrolase family protein [Streptococcus pneumoniae S... gi\|148996986, gi... - gi\|148996986\|ref\|ZP_01824640.1\| carbon-nitrogen hydrolase family protein [Streptococcus pneumoniae S... gi\|147923055, gi... - gi\|148984664\|ref\|ZP_01817932.1\| carbon-nitrogen hydrolase family protein [Streptococcus pneumoniae S... gi\|242236240, gi... - gi\|242245204\|ref\|ZP_04799641.1\| N-carbamoylputrescine amidase [Streptococcus pneumoniae TCH8431/19A]... gi\|237821877 - gi\|237821877\|ref\|ZP_04597722.1\| N-carbamoylputrescine amidase [Streptococcus pneumoniae CCRI 1974M2] gi\|237651125 - gi\|237651125\|ref\|ZP_04525377.1\| N-carbamoylputrescine amidase [Streptococcus pneumoniae CCRI 1974]	3.0E-68	[4..306]	[2..286]
AGUB_SOLLC - N-carbamoylputrescine amidase OS=Solanum lycopersicum GN=CPA PE=2 SV=1	5.0E-68	[4..306]	[6..287]

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Predicted Domain #1

Region A:
Residues: [1-307]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTSKLKRVAV AQLCSSADLT KNLKVVKELI SEAIQKKADV VFLPEASDYL SQNPLHSRYL  60
   61 AQKSPKFIRQ LQSSITDLVR DNSRNIDVSI GVHLPPSEQD LLEGNDRVRN VLLYIDHEGK 120
  121 ILQEYQKLHL FDVDVPNGPI LKESKSVQPG KAIPDIIESP LGKLGSAICY DIRFPEFSLK 180
  181 LRSMGAEILC FPSAFTIKTG EAHWELLGRA RAVDTQCYVL MPGQVGMHDL SDPEWEKQSH 240
  241 MSALEKSSRR ESWGHSMVID PWGKIIAHAD PSTVGPQLIL ADLDRELLQE IRNKMPLWNQ 300
  301 RRDDLFH

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	552.522879
Match:	1emsA_
Description:	NIT-FHIT fusion protein, C-terminal domain; NIT-FHIT fusion protein, N-terminal domain
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
catalytic activity	1.64442398754697	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	1.35962310800332	bayes_pls_golite062009
N-carbamoylputrescine amidase activity	0.749539597459006	bayes_pls_golite062009
hydrolase activity	0.06604196264626	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: