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View Structure Prediction Details

Protein: PUF4
Organism: Saccharomyces cerevisiae
Length: 888 amino acids
Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for PUF4.

Description E-value Query
Range
Subject
Range
gi|4261598, gi|2... - gi|4261598|gb|AAD13898.1|S58126_1111111 Unknown [Saccharomyces cerevisiae], gi|234327|gb|AAB19616.1|...
661.0 [0..1] [888..1]
PUM3_ARATH - Pumilio homolog 3 OS=Arabidopsis thaliana GN=APUM3 PE=1 SV=1
543.0 [0..71] [884..103]
PUM2_MOUSE - Pumilio homolog 2 OS=Mus musculus GN=Pum2 PE=1 SV=2
496.0 [0..3] [884..174]
gi|114576341, gi... - gi|114576341|ref|XP_001140758.1| PREDICTED: pumilio homolog 2 isoform 11 [Pan troglodytes], gi|11457...
494.0 [0..3] [884..175]
gi|109102137, gi... - gi|109102137|ref|XP_001095963.1| PREDICTED: similar to pumilio homolog 2 isoform 4 [Macaca mulatta],...
494.0 [0..3] [884..175]

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Predicted Domain #1
Region A:
Residues: [1-89]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSTKGLKEEI DDVPSVDPVV SETVNSALEQ LQLDDPEENA TSNAFANKVS QDSQFANGPP  60
   61 SQMFPHPQMM GGMGFMPYSQ MMQVPHNPC

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

    MCM
Score
SCOP
Match
SCOP Description
View Download 0.694 a.74.1 Cyclin-like
View Download 0.694 a.74.1 Cyclin-like
View Download 0.678 a.74.1 Cyclin-like
View Download 0.678 a.74.1 Cyclin-like
View Download 0.655 a.74.1 Cyclin-like
View Download 0.655 a.74.1 Cyclin-like
View Download 0.568 d.94.1 HPr-like
View Download 0.560 a.74.1 Cyclin-like
View Download 0.560 a.74.1 Cyclin-like

Predicted Domain #2
Region A:
Residues: [90-216]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PFFPPPDFND PTAPLSSSPL NAGGPPMLFK NDSLPFQMLS SGAAVATQGG QNLNPLINDN  60
   61 SMKVLPIASA DPLWTHSNVP GSASVAIEET TATLQESLPS KGRESNNKAS SFRRQTFHAL 120
  121 SPTDLIN

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 3.09691
Match: 1deqA
Description: Fibrinogen
Matching Structure (courtesy of the PDB):

Predicted Domain #3
Region A:
Residues: [217-446]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 AANNVTLSKD FQSDMQNFSK AKKPSVGANN TAKTRTQSIS FDNTPSSTSF IPPTNSVSEK  60
   61 LSDFKIETSK EDLINKTAPA KKESPTTYGA AYPYGGPLLQ PNPIMPGHPH NISSPIYGIR 120
  121 SPFPNSYEMG AQFQPFSPIL NPTSHSLNAN SPIPLTQSPI HLAPVLNPSS NSVAFSDMKN 180
  181 DGGKPTTDND KAGPNVRMDL INPNLGPSMQ PFHILPPQQN TPPPPWLYST 

[Run NCBI BLAST on this sequence.]

Detection Method: ab initio
Confidence: 11.96
Match:
Description: No description for 1deqA was found.

Predicted Domain #4
Region A:
Residues: [447-521]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PPPFNAMVPP HLLAQNHMPL MNSANNKHHG RNNNSMSSHN DNDNIGNSNY NNKDTGRSNV  60
   61 GKMKNMKNSY HGYYN

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

    MCM
Score
SCOP
Match
SCOP Description
View Download 0.338 a.60.11 Hypothetical protein YjbJ
View Download 0.334 a.28.1 ACP-like
View Download 0.307 a.23.1 HSC20 (HSCB), C-terminal oligomerisation domain
View Download 0.307 a.23.1 HSC20 (HSCB), C-terminal oligomerisation domain
View Download 0.299 a.80.1 DNA polymerase III clamp loader subunits, C-terminal domain
View Download 0.296 d.50.3 PI-Pfui intein middle domain

Predicted Domain #5
Region A:
Residues: [522-673]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NNNNNNNNNN NNNNSNATNS NSAEKQRKIE ESSRFADAVL DQYIGSIHSL CKDQHGCRFL  60
   61 QKQLDILGSK AADAIFEETK DYTVELMTDS FGNYLIQKLL EEVTTEQRIV LTKISSPHFV 120
  121 EISLNPHGTR ALQKLIECIK TDEEAQIVVD SL

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 624.228787
Match: 1ib2A
Description: Pumilio 1
Matching Structure (courtesy of the PDB):

Predicted Domain #6
Region A:
Residues: [674-776]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 RPYTVQLSKD LNGNHVIQKC LQRLKPENFQ FIFDAISDSC IDIATHRHGC CVLQRCLDHG  60
   61 TTEQCDNLCD KLLALVDKLT LDPFGNYVVQ YIITKEAEKN KYD

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 624.228787
Match: 1ib2A
Description: Pumilio 1
Matching Structure (courtesy of the PDB):

Predicted Domain #7
Region A:
Residues: [777-888]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 YTHKIVHLLK PRAIELSIHK FGSNVIEKIL KTAIVSEPMI LEILNNGGET GIQSLLNDSY  60
   61 GNYVLQTALD ISHKQNDYLY KRLSEIVAPL LVGPIRNTPH GKRIIGMLHL DS

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 624.228787
Match: 1ib2A
Description: Pumilio 1
Matching Structure (courtesy of the PDB):

YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle