YRC Public Data Repository - Protein Structure Prediction

Protein:	SAK1
Organism:	Saccharomyces cerevisiae
Length:	1142 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for SAK1.

Description	E-value	Query Range	Subject Range
SAK1_YEAST - SNF1-activating kinase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SAK1 PE=1 SV=1 SAK1 - Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; mem...	0.0	[1..1142]	[1..1142]
CDPKL_ARATH - Calcium-dependent protein kinase 21 OS=Arabidopsis thaliana GN=CPK21 PE=1 SV=1	0.0	[61..637]	[5..525]
gi\|16215467 - gi\|16215467\|emb\|CAC82998.1\| calcium-dependent protein kinase 2 [Nicotiana tabacum]	0.0	[16..646]	[8..571]
gi\|16215475 - gi\|16215475\|emb\|CAC83000.1\| calcium-dependent protein kinase 2 [Nicotiana benthamiana]	0.0	[16..646]	[8..571]
gi\|15289758 - gi\|15289758\|dbj\|BAB63463.1\| calcium dependent protein kinase [Solanum tuberosum]	0.0	[16..646]	[8..568]
CDPK2_ORYSJ - Calcium-dependent protein kinase isoform 2 OS=Oryza sativa subsp. japonica GN=CPK2 PE=2 SV=2 CDPK2_ORYSA - Calcium-dependent protein kinase, isoform 2 (EC 2.7.11.1) (CDPK 2) - Oryza sativa (Rice)	0.0	[27..634]	[2..527]

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Predicted Domain #1

Region A:
Residues: [1-96]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDRSDKKVNV EEVNVPSNLQ IELEKSGTSS SVSLRSPTKS SATNLAGMAE GARDNASIAS  60
   61 SSVDSLNMLL ERQRVRQLNH PQHQQHISSS LAKTPT

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

		MCM Score	SCOP Match	SCOP Description
View	Download	0.528	a.24.13	Domain of the SRP/SRP receptor G-proteins
View	Download	0.666	a.7.6	Ribosomal protein S20
View	Download	0.594	a.80.1	DNA polymerase III clamp loader subunits, C-terminal domain
View	Download	0.558	a.48.3	Conserved domain common to transcription factors TFIIS, elongin A, CRSP70
View	Download	0.666	a.7.6	Ribosomal protein S20
View	Download	0.594	a.80.1	DNA polymerase III clamp loader subunits, C-terminal domain
View	Download	0.558	a.48.3	Conserved domain common to transcription factors TFIIS, elongin A, CRSP70
View	Download	0.528	a.24.13	Domain of the SRP/SRP receptor G-proteins
View	Download	0.521	c.55.7	Methylated DNA-protein cysteine methyltransferase domain
View	Download	0.521	c.55.7	Methylated DNA-protein cysteine methyltransferase domain
View	Download	0.510	a.130.1	Chorismate mutase II
View	Download	0.501	a.118.1	ARM repeat
View	Download	0.498	b.40.2	Bacterial enterotoxins
View	Download	0.466	a.118.1	ARM repeat
View	Download	0.426	b.40.4	Nucleic acid-binding proteins
View	Download	0.425	d.16.1	FAD-linked reductases, C-terminal domain
View	Download	0.422	b.71.1	Glycosyl hydrolase domain
View	Download	0.421	a.24.13	Domain of the SRP/SRP receptor G-proteins
View	Download	0.416	b.61.3	D-aminopeptidase, middle and C-terminal domains
View	Download	0.401	b.1.1	Immunoglobulin
View	Download	0.401	a.81.1	N-terminal domain of DnaB helicase
View	Download	0.400	b.71.1	Glycosyl hydrolase domain
View	Download	0.398	d.199.1	DNA-binding C-terminal domain of the transcription factor MotA
View	Download	0.395	b.36.1	PDZ domain-like
View	Download	0.393	b.33.1	ISP domain
View	Download	0.392	a.27.1	Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases
View	Download	0.383	c.55.7	Methylated DNA-protein cysteine methyltransferase domain
View	Download	0.382	f.1.4	Bcl-2 inhibitors of programmed cell death
View	Download	0.381	d.58.39	Glutamyl tRNA-reductase catalytic, N-terminal domain
View	Download	0.375	d.58.26	GHMP Kinase, C-terminal domain
View	Download	0.363	a.24.10	Histidine-containing phosphotransfer domain, HPT domain
View	Download	0.363	a.52.1	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin
View	Download	0.359	a.39.1	EF-hand
View	Download	0.353	d.16.1	FAD-linked reductases, C-terminal domain
View	Download	0.347	b.55.1	PH domain-like
View	Download	0.331	a.47.2	t-snare proteins
View	Download	0.328	b.40.4	Nucleic acid-binding proteins
View	Download	0.327	a.61.1	Retroviral matrix proteins
View	Download	0.327	b.71.1	Glycosyl hydrolase domain
View	Download	0.317	c.55.3	Ribonuclease H-like
View	Download	0.312	a.1.1	Globin-like
View	Download	0.312	a.24.17	Group V grass pollen allergen
View	Download	0.309	d.19.1	MHC antigen-recognition domain
View	Download	0.309	d.34.1	DNA-binding domain of Mlu1-box binding protein MBP1
View	Download	0.302	a.27.1	Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases
View	Download	0.298	a.24.15	FAD-dependent thiol oxidase
View	Download	0.292	d.4.1	His-Me finger endonucleases
View	Download	0.290	a.138.1	Multiheme cytochromes
View	Download	0.288	b.61.4	Quinohemoprotein amine dehydrogenase A chain, domain 3
View	Download	0.285	i.1.1	Ribosome and ribosomal fragments
View	Download	0.282	a.46.1	Methionine synthase domain
View	Download	0.281	d.224.1	SufE-like
View	Download	0.279	d.87.2	CO dehydrogenase flavoprotein C-terminal domain-like
View	Download	0.277	b.71.1	Glycosyl hydrolase domain
View	Download	0.276	a.7.1	Spectrin repeat
View	Download	0.272	a.24.4	Hemerythrin
View	Download	0.270	a.5.4	Elongation factor TFIIS domain 2
View	Download	0.263	a.52.1	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin
View	Download	0.261	a.4.5	"Winged helix" DNA-binding domain
View	Download	0.260	d.19.1	MHC antigen-recognition domain
View	Download	0.249	a.11.2	Second domain of FERM
View	Download	0.249	d.13.1	HIT-like
View	Download	0.247	b.61.3	D-aminopeptidase, middle and C-terminal domains
View	Download	0.246	a.80.1	DNA polymerase III clamp loader subunits, C-terminal domain
View	Download	0.238	a.60.5	Barrier-to-autointegration factor, BAF
View	Download	0.237	d.52.5	Probable GTPase Der, C-terminal domain
View	Download	0.236	f.36.1	Neurotransmitter-gated ion-channel pransmembrane pore
View	Download	0.235	d.87.1	FAD/NAD-linked reductases, dimerisation (C-terminal) domain
View	Download	0.235	a.133.1	Phospholipase A2, PLA2
View	Download	0.232	d.54.1	Enolase N-terminal domain-like
View	Download	0.232	a.45.1	Glutathione S-transferase (GST), C-terminal domain
View	Download	0.229	d.110.4	SNARE-like
View	Download	0.224	a.12.1	Kix domain of CBP (creb binding protein)
View	Download	0.221	d.74.3	RBP11-like subunits of RNA polymerase
View	Download	0.220	c.58.1	Aminoacid dehydrogenase-like, N-terminal domain
View	Download	0.218	d.14.1	Ribosomal protein S5 domain 2-like
View	Download	0.211	d.41.1	CO dehydrogenase molybdoprotein N-domain-like
View	Download	0.211	a.61.1	Retroviral matrix proteins
View	Download	0.209	d.16.1	FAD-linked reductases, C-terminal domain

Predicted Domain #2

Region A:
Residues: [97-228]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 TTSSFCSSGS SKNKVKETNR ISLTYDPVSK RKVLNTYEII KELGHGQHGK VKLARDILSK  60
   61 QLVAIKIVDR HEKKQRKFFT FIKSSKISEN DKIKREIAIM KKCHHKHVVQ LIEVLDDLKS 120
  121 RKIYLVLEYC SR

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [473-496]

      1          11         21         31         41         51         
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    1 FQCNQTDQFE PISISKHELK NAVS

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	350.70927
Match:	1a06__
Description:	Calmodulin-dependent protein kinase
Matching Structure (courtesy of the PDB):

Predicted Domain #3

Region A:
Residues: [229-472]

      1          11         21         31         41         51         
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    1 GEVKWCPPDC MESDAKGPSL LSFQETREIL RGVVLGLEYL HYQGIIHRDI KPANLLISGD  60
   61 GTVKISDFGV SLAASSTNSS DSSESLDELE LAKTVGTPAF FAPEMCLGED AFTRYNLTKE 120
  121 NLFRGSCISF MIDIWAVGVT LYCLLFGMLP FFSDFELKLF EKIVNDPLKF PTFKEIQSNK 180
  181 VSKVSCEEEY EMAKDLLLKL LEKNPQKRMT IPAIKKHPFV SWDFDHVPEN DEKLLSSVLE 240
  241 QKLR

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	350.70927
Match:	1a06__
Description:	Calmodulin-dependent protein kinase
Matching Structure (courtesy of the PDB):

Predicted Domain #4

Region A:
Residues: [497-569]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 GVGKKIKESV LKSIPLKDPS DLSNKNYLHP TETTRGRGDA NVIVSEGSVL SNIKELSAND  60
   61 GCLNTDSDTN INI

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	21.0
Match:	1iq5A_
Description:	Calmodulin
Matching Structure (courtesy of the PDB):

Predicted Domain #5

Region A:
Residues: [570-644]

      1          11         21         31         41         51         
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    1 NDDDHYSGDD NDGHLTKREL ERELNKFDDK HEAGNMVNLP INSSFASLDS FYIDNFAMAR  60
   61 MGMSSPEAGD SVSSV

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	21.0
Match:	1iq5A_
Description:	Calmodulin
Matching Structure (courtesy of the PDB):

Predicted Domain #6

Region A:
Residues: [645-796]

      1          11         21         31         41         51         
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    1 PNLPSAPSST RLGRSPVFSG VTNQPSPIRP VLPQQKSSFC ATGRYDKSHN SLLRNSSSHL  60
   61 TSYNSGRPSS RTGRMNSRNQ NLPKIPNSLS KISTTKLTEL RVPKDSEIPS PAKNPNADRL 120
  121 RRFPVKKNTK TPAIKDPPRI NINSSDKSGS KN

[Run NCBI BLAST on this sequence.]

Detection Method:	ORFEUS
Confidence:	7.78
Match:	1jl5A
Description:	Leucine rich effector protein YopM
Matching Structure (courtesy of the PDB):

Predicted Domain #7

Region A:
Residues: [797-1142]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SPIKSLYQRM KQSKDNSKTF EVRRGNFFSH FNGDDDDSSS QSSVTSSGSE SDSELSSTSS  60
   61 SCTSGTQSRN SSNNNAYSET ESLPFEFGVD SEDGSGVLLR DLPNEDQIRP FLDIQPCRRM 120
  121 KVKSSLNLEP PSVSSSSSSS SDEDELILNV GTAGHRRRHN SSKLSELSNS PQKGSNNFMY 180
  181 SNGSVHDSET TITPQNMDDL TLHQALSRSQ PISKPGPLVL PKRLDQKKAT TETSNLTDIV 240
  241 EFNGNNDHRK DKNFDKVLYS RDLLKDALSS TNAGRRRSIP SNKIRGRKDA SITMSTNVGN 300
  301 DEHARNTSCH GDKGQENGAI KQRTHERSRS LTVAELNEEK RRSALP

[Run NCBI BLAST on this sequence.]

Detection Method:	ORFEUS
Confidence:	7.78
Match:	1jl5A
Description:	Leucine rich effector protein YopM
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits