YRC Public Data Repository - Protein Structure Prediction

Protein:	ASP1
Organism:	Saccharomyces cerevisiae
Length:	381 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for ASP1.

Description	E-value	Query Range	Subject Range
gi\|207346453 - gi\|207346453\|gb\|EDZ72942.1\| YDR321Wp-like protein [Saccharomyces cerevisiae AWRI1631] ASPG1_YEAST - L-asparaginase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ASP1 PE=1 SV=1 ASP1 - Cytosolic L-asparaginase, involved in asparagine catabolism gi\|190404738 - gi\|190404738\|gb\|EDV08005.1\| L-asparaginase I [Saccharomyces cerevisiae RM11-1a] gi\|151942297 - gi\|151942297\|gb\|EDN60653.1\| asparaginase I [Saccharomyces cerevisiae YJM789]	0.0	[1..381]	[1..381]
GATD_PYRHO - Glutamyl-tRNA(Gln) amidotransferase subunit D OS=Pyrococcus horikoshii (strain ATCC 700860 / DSM 124... GATD_PYRHO - Glutamyl-tRNA(Gln) amidotransferase subunit D OS=Pyrococcus horikoshii GN=gatD PE=3 SV=1	0.0	[10..381]	[46..425]
GATD_PYRFU - Glutamyl-tRNA(Gln) amidotransferase subunit D OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 /... GATD_PYRFU - Glutamyl-tRNA(Gln) amidotransferase subunit D OS=Pyrococcus furiosus GN=gatD PE=3 SV=1	0.0	[10..381]	[46..425]
GATD_PYRAB - Glutamyl-tRNA(Gln) amidotransferase subunit D OS=Pyrococcus abyssi (strain GE5 / Orsay) GN=gatD PE=1... GATD_PYRAB - Glutamyl-tRNA(Gln) amidotransferase subunit D OS=Pyrococcus abyssi GN=gatD PE=1 SV=1	0.0	[10..381]	[46..425]
gi\|7451280, gi\|6... - gi\|7451280\|pir\|\|C69194 L-asparaginase I - Methanobacterium thermoautotrophicum (strain Delta H), gi... GATD_METTH - Glutamyl-tRNA(Gln) amidotransferase subunit D OS=Methanothermobacter thermautotrophicus (strain ATCC...	0.0	[2..381]	[38..422]
gi\|189363657, gi... - gi\|189363657\|gb\|EDU82076.1\| L-asparaginase 2 [Escherichia coli O157:H7 str. EC4486], gi\|168766964\|re... gi\|189369341, gi... - gi\|189369341\|gb\|EDU87757.1\| L-asparaginase 2 [Escherichia coli O157:H7 str. EC4501], gi\|168760099\|re... gi\|189358583, gi... - gi\|189358583\|gb\|EDU77002.1\| L-asparaginase 2 [Escherichia coli O157:H7 str. EC4401], gi\|168753909\|re... gi\|168747551, gi... - gi\|188017927\|gb\|EDU56049.1\| L-asparaginase 2 [Escherichia coli O157:H7 str. EC4113], gi\|168747551\|re... gi\|217327513, gi... - gi\|217327513\|ref\|ZP_03443596.1\| L-asparaginase 2 [Escherichia coli O157:H7 str. TW14588], gi\|2173198... gi\|209158459, gi... - gi\|209397059\|ref\|YP_002272437.1\| L-asparaginase 2 [Escherichia coli O157:H7 str. EC4115], gi\|2091584... gi\|15833087, gi\|... - gi\|15833087\|ref\|NP_311860.1\| L-asparaginase II [Escherichia coli O157:H7 str. Sakai], gi\|13363305\|db... gi\|189379870, gi... - gi\|189379870\|gb\|EDU98286.1\| L-asparaginase 2 [Escherichia coli O157:H7 str. EC508], gi\|168797532\|ref... gi\|189374047, gi... - gi\|189374047\|gb\|EDU92463.1\| L-asparaginase 2 [Escherichia coli O157:H7 str. EC869], gi\|168785815\|ref... gi\|168781816, gi... - gi\|189000626\|gb\|EDU69612.1\| L-asparaginase 2 [Escherichia coli O157:H7 str. EC4076], gi\|168781816\|re... gi\|187770804, gi... - gi\|187770804\|gb\|EDU34648.1\| L-asparaginase 2 [Escherichia coli O157:H7 str. EC4196], gi\|168773404\|re... gi\|15833087, gi\|... - gi\|15833087\|ref\|NP_311860.1\| periplasmic L-asparaginase II [Escherichia coli O157:H7], gi\|13363305\|d... gi\|208820500, gi... - gi\|208820500\|ref\|ZP_03260820.1\| L-asparaginase 2 [Escherichia coli O157:H7 str. EC4042], gi\|20874062... gi\|25290105, gi\|... - pir\|\|D85953 periplasmic L-asparaginase II [imported] - Escherichia coli (strain O157:H7, substrain ... gi\|208734271, gi... - gi\|208812994\|ref\|ZP_03254323.1\| L-asparaginase 2 [Escherichia coli O157:H7 str. EC4045], gi\|20873427... gi\|254594309, gi... - gi\|254794909\|ref\|YP_003079746.1\| periplasmic L-asparaginase II [Escherichia coli O157:H7 str. TW1435... gi\|208727388, gi... - gi\|208807587\|ref\|ZP_03249924.1\| L-asparaginase 2 [Escherichia coli O157:H7 str. EC4206], gi\|20872738... gi\|15803496, gi\|... - gi\|15803496\|ref\|NP_289529.1\| L-asparaginase II [Escherichia coli O157:H7 EDL933], gi\|12517506\|gb\|AAG... gi\|195937095 - gi\|195937095\|ref\|ZP_03082477.1\| L-asparaginase II [Escherichia coli O157:H7 str. EC4024]	0.0	[32..377]	[2..347]
gi\|668327 - gi\|668327\|gb\|AAA62276.1\| chimeric immunoglobulin-L-asparaginase [synthetic construct]	0.0	[51..377]	[288..614]
gi\|238902079, gi... - gi\|238902079\|ref\|YP_002927875.1\| periplasmic L-asparaginase II [Escherichia coli BW2952], gi\|2388620... gi\|253772205, gi... - gi\|253772205\|ref\|YP_003035036.1\| L-asparaginase, type II [Escherichia coli BL21(DE3)], gi\|253323249\|... ASG2_ECOLI, ASPG... - L-asparaginase 2 precursor (EC 3.5.1.1) (L-asparaginase II) (L-asparagine amidohydrolase II) (L-ASNa... EG10046 - periplasmic L-asparaginase 2 / L-asparaginase 2, periplasmic, high affinity EG10046 - periplasmic L-asparaginase 2 / L-asparaginase 2, periplasmic, high affinity gi\|85675767, gi\|... - gi\|89109734\|ref\|AP_003514.1\| periplasmic L-asparaginase II [Escherichia coli str. K-12 substr. W3110... gi\|169890344, gi... - gi\|170082509\|ref\|YP_001731829.1\| periplasmic L-asparaginase II [Escherichia coli str. K-12 substr. D...	0.0	[32..377]	[2..347]
gi\|213850154 - gi\|213850154\|ref\|ZP_03381052.1\| L-asparaginase II [Salmonella enterica subsp. enterica serovar Typhi... gi\|213648212 - gi\|213648212\|ref\|ZP_03378265.1\| L-asparaginase II [Salmonella enterica subsp. enterica serovar Typhi... gi\|224584899, gi... - gi\|224584899\|ref\|YP_002638698.1\| L-asparaginase [Salmonella enterica subsp. enterica serovar Paratyp... gi\|214001049 - gi\|214001049\|ref\|ZP_03411784.1\| L-asparaginase II [Salmonella enterica subsp. enterica serovar Typhi... gi\|213051774 - gi\|213051774\|ref\|ZP_03344652.1\| L-asparaginase II [Salmonella enterica subsp. enterica serovar Typhi... gi\|204928057, gi... - gi\|204928057\|ref\|ZP_03219257.1\| L-asparaginase 2 [Salmonella enterica subsp. enterica serovar Javian... gi\|213609095 - gi\|213609095\|ref\|ZP_03368921.1\| L-asparaginase II [Salmonella enterica subsp. enterica serovar Typhi... gi\|213424103 - gi\|213424103\|ref\|ZP_03356996.1\| L-asparaginase II [Salmonella enterica subsp. enterica serovar Typhi... gi\|197095450, gi... - gi\|197363973\|ref\|YP_002143610.1\| L-asparaginase II [Salmonella enterica subsp. enterica serovar Para... gi\|197248611, gi... - gi\|197248611\|ref\|YP_002148020.1\| L-asparaginase 2 [Salmonella enterica subsp. enterica serovar Agona... gi\|199603918, gi... - gi\|200386820\|ref\|ZP_03213432.1\| L-asparaginase 2 [Salmonella enterica subsp. enterica serovar Vircho... gi\|198241840, gi... - gi\|198241840\|ref\|YP_002217081.1\| L-asparaginase 2 [Salmonella enterica subsp. enterica serovar Dubli... lcl\|NC_016856.1_... - [gene=ansB] [protein=L-asparaginase II] [protein_id=YP_005239252.1] [location=complement(3284603..32... gi\|29139002, gi\|... - gi\|29143368\|ref\|NP_806710.1\| L-asparaginase II [Salmonella enterica subsp. enterica serovar Typhi st... gi\|16761881 - gi\|16761881\|ref\|NP_457498.1\| L-asparaginase II [Salmonella enterica subsp. enterica serovar Typhi st... gi\|25290114, gi\|... - gi\|25290114\|pir\|\|AB0879 asparaginase (EC 3.5.1.1) - Salmonella enterica subsp. enterica serovar Typh... gi\|16421659, gi\|... - gi\|16766407\|ref\|NP_462022.1\| L-asparaginase II [Salmonella typhimurium LT2], gi\|16421659\|gb\|AAL21981... gi\|56129301, gi\|... - gi\|56415044\|ref\|YP_152119.1\| L-asparaginase II [Salmonella enterica subsp. enterica serovar Paratyph... gi\|205347033, gi... - gi\|205347033\|gb\|EDZ33664.1\| L-asparaginase 2 [Salmonella enterica subsp. enterica serovar Hadar str.... gi\|168234343, gi... - gi\|205331688\|gb\|EDZ18452.1\| L-asparaginase 2 [Salmonella enterica subsp. enterica serovar Kentucky s... gi\|194455085, gi... - gi\|194468721\|ref\|ZP_03074705.1\| L-asparaginase 2 [Salmonella enterica subsp. enterica serovar Kentuc... gi\|238909904 - gi\|238909904\|ref\|ZP_04653741.1\| L-asparaginase II [Salmonella enterica subsp. enterica serovar Tenne... gi\|194404359, gi... - gi\|194445696\|ref\|YP_002042365.1\| L-asparaginase 2 [Salmonella enterica subsp. enterica serovar Newpo... gi\|205331124, gi... - gi\|205331124\|gb\|EDZ17888.1\| L-asparaginase 2 [Salmonella enterica subsp. enterica serovar 4,[5],12:i... gi\|167552002, gi... - gi\|205323300\|gb\|EDZ11139.1\| L-asparaginase 2 [Salmonella enterica subsp. enterica serovar Saintpaul ...	0.0	[32..377]	[2..347]
gi\|2392782, gi\|2... - gi\|2392785\|pdb\|4ECA\|D Chain D, Asparaginase From E. Coli, Mutant T89v With Covalently Bound Aspartat...	0.0	[53..377]	[1..325]

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Predicted Domain #1

Region A:
Residues: [1-264]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKSDSVEITT ICPDVENSQF VVQSNCPETI PEILKSQNAA VNGSGIACQQ RSLPRIKILG  60
   61 TGGTIASKAI DSSQTAGYHV DLTIQDLLDA IPDISKVCDI EYEQLCNVDS KDINEDILYK 120
  121 IYKGVSESLQ AFDGIVITHG TDTLSETAFF IESTIDAGDV PIVFVGSMRP STSVSADGPM 180
  181 NLYQAICIAS NPKSRGRGVL VSLNDQISSG YYITKTNANS LDSFNVRQGY LGNFVNNEIH 240
  241 YYYPPVKPQG CHKFKLRVDG KHFK

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	609.9897
Match:	1hfjA_
Description:	Asparaginase type II
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
asparaginase activity	5.31552786314061	bayes_pls_golite062009
hydrolase activity	4.24082880889802	bayes_pls_golite062009
catalytic activity	1.35076351157997	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	0.739997194025722	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides	0.584091474722373	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [265-381]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LPEVCILYAH QAFPPAIVNL VADKYDGIVL ATMGAGSLPE EVNETCMKLS LPIVYSKRSM  60
   61 DGMVPIANVP KKGSKEDNLI ASGYLSPEKS RILLQLCLAG NYTLEEIKHV FTGVYGG

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	609.9897
Match:	1hfjA_
Description:	Asparaginase type II
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: