YRC Public Data Repository - Protein Structure Prediction

Protein:	THI13
Organism:	Saccharomyces cerevisiae
Length:	340 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for THI13.

Description	E-value	Query Range	Subject Range
gi\|157069916, gi... - gi\|164423028\|ref\|XP_001728013.1\| nmt1 protein, variant 2 [Neurospora crassa OR74A], gi\|164423026\|ref... gi\|15822513 - gi\|15822513\|gb\|AAG23338.1\| thiamine biosynthesis protein NMT-1 [Neurospora crassa]	5.0E-78	[1..338]	[1..338]
THI13_YEAST - 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase THI13 OS=Saccharomyces cerevisiae (str... gi\|151945223, gi... - gi\|151945223\|gb\|EDN63474.1\| thiamine metabolism-related protein [Saccharomyces cerevisiae YJM789], g... THI13 - Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a s... gi\|190409294, gi... - gi\|190409294\|gb\|EDV12559.1\| pyrimidine precursor biosynthesis enzyme THI13 [Saccharomyces cerevisiae...	5.0E-78	[1..340]	[1..340]
gi\|5777326 - gi\|5777326\|dbj\|BAA83524.1\| pyrimidine precursor biosynthesis enzyme [Candida tropicalis]	5.0E-74	[1..338]	[1..338]
NMT1_ASPPA - 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase OS=Aspergillus parasiticus GN=nmt1 PE=...	5.0E-72	[1..336]	[1..336]
gi\|3282220 - gi\|3282220\|gb\|AAC64375.1\| thiamine synthase homolog [Botryotinia fuckeliana] gi\|154316628, gi... - gi\|154316628\|ref\|XP_001557635.1\| conserved hypothetical protein [Botryotinia fuckeliana B05.10], gi\|...	5.0E-70	[1..336]	[1..336]
NMT1_SCHPO - 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase OS=Schizosaccharomyces pombe (strain 9... nmt1 - no message in thiamine Nmt1	4.0E-68	[1..332]	[1..331]
NMT1_UROFA - 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase OS=Uromyces fabae GN=PIG1 PE=2 SV=1	1.0E-64	[1..332]	[1..334]

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Predicted Domain #1

Region A:
Residues: [1-87]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSTDKITFLL NWQPTPYHIP IFLAQTKGYF KEQGLDIAIL EPTNPSDVTE LIGSGKVDMG  60
   61 LKAMIHTLAA KARGFPVTSV ASLLDEP

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [186-288]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 RIDKLACLGC CCFCTVLYIC NDEFLKKNPE KVRKFLKAIK KATDYVLADP VKAWKEYIDF  60
   61 KPQLNNDLSY KQYQRCYAYF SSSLYNVHRD WKKVTGYGKR LAI

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	25.522879
Match:	1gggA_
Description:	Glutamine-binding protein
Matching Structure (courtesy of the PDB):

Predicted Domain #2

Region A:
Residues: [88-185]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 FTGVLYLKGS GITEDFQSLK GKKIGYVGEF GKIQIDELTK HYGMKPEDYT AVRCGMNVAK  60
   61 YIIEGKIDAG IGIECMQQVE LEEYLAKQGR PASDAKML

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	25.522879
Match:	1gggA_
Description:	Glutamine-binding protein
Matching Structure (courtesy of the PDB):

Predicted Domain #3

Region A:
Residues: [289-340]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LPPDYVSNYT NEYLSWPEPE EVSDPLEAQR LMAIHQEKCR QEGTFKRLAL PA

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits