Bait Protein: | RLF2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Green EM, Antczak AJ, Bailey AO, Franco AA, Wu KJ, Yates JR 3rd, Kaufman PD. Replication-independent histone deposition by the HIR complex and Asf1. Curr Biol. 2005 Nov 22;15(22):2044-9. |
Comments: | identification data - first search in cascade |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
MSI1 | Subunit of chromatin assembly factor I (CAF-1), negative regulator of the RAS/cAMP pathway via seque... | 53 | 493 | 79.6% | 0.0906 | 47365 |
[ |
RLF2 | Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1) with Cac2p and Msi1p that assembles ... | 62 | 398 | 59.1% | 0.0509 | 70210 |
[ |
CAC2 | Component of the chromatin assembly complex (with Rlf2p and Msi1p) that assembles newly synthesized ... | 39 | 332 | 62.6% | 0.055 | 51252 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 17 | 97 | 38.6% | 0.0164 | 50033 |
[ |
RNQ1 | [PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate | 16 | 48 | 26.9% | 0.00919 | 42580 |
[ |
HHT1, HHT2 | Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated t... | 6 | 23 | 28.7% | 0.0131 | 15356 |
[ |
HTA2 | Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of tw... | 6 | 18 | 39.4% | 0.0106 | 13989 |
[ |
HTA1 | Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of tw... | 6 | 18 | 39.4% | 0.0106 | 13989 |
[ |
RPP0 | Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphoryl... | 6 | 16 | 22.8% | 0.00398 | 33717 |
[ |
DED1 | ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... | 5 | 15 | 14.9% | 0.00193 | 65553 |
[ |
HHF1, HHF2 | Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two... | 3 | 14 | 28.2% | 0.0105 | 11368 |
[ |
HTB2 | Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly id... | 3 | 12 | 26.0% | 0.00711 | 14237 |
[ |
HTB1 | Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly id... | 3 | 12 | 26.0% | 0.00711 | 14252 |
[ |
STH1 | ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic g... | 7 | 12 | 7.9% | 6.85E-4 | 156742 |
[ |
NCP1 | NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately reg... | 4 | 11 | 10.0% | 0.00123 | 76772 |
[ |
RPP1A | Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation facto... | 3 | 10 | 49.1% | 0.00732 | 10908 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 5 | 10 | 9.9% | 8.45E-4 | 99619 |
[ |
FUN12 | GTPase, required for general translation initiation by promoting Met-tRNAiMet binding to ribosomes a... | 5 | 10 | 9.6% | 7.74E-4 | 112268 |
[ |
RSC58 | Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and e... | 5 | 9 | 19.5% | 0.00139 | 57795 |
[ |
RSC8 | Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homol... | 4 | 9 | 11.1% | 0.00125 | 63168 |
[ |
DEF1 | RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and prot... | 5 | 9 | 9.9% | 9.46E-4 | 83973 |
[ |
SUM1 | Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also... | 6 | 8 | 10.3% | 5.84E-4 | 118201 |
[ |
RPP2B | Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... | 2 | 6 | 32.7% | 0.00423 | 11050 |
[ |
PMA2 | Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator ... | 4 | 6 | 7.3% | 4.91E-4 | 102172 |
[ |
FKS1 | Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... | 6 | 6 | 6.4% | 2.48E-4 | 214849 |
[ |
TOP2 | Essential type II topoisomerase, relieves torsional strain in DNA by cleaving and re-sealing the pho... | 5 | 6 | 5.7% | 3.26E-4 | 164214 |
[ |
RVB1 | Essential protein involved in transcription regulation; component of chromatin remodeling complexes;... | 3 | 5 | 12.1% | 8.38E-4 | 50453 |
[ |
NPL6 | Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Ldb7p, and Htl1p to... | 2 | 5 | 10.8% | 8.92E-4 | 49651 |
[ |
PET9 | Major ADP/ATP carrier of the mitochondrial inner membrane, exchanges cytosolic ADP for mitochondrial... | 2 | 5 | 10.4% | 0.00122 | 34426 |
[ |
YHL008C | Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appea... | 2 | 5 | 6.9% | 6.19E-4 | 69969 |
[ |
RGA1 | GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of se... | 2 | 5 | 2.4% | 3.85E-4 | 112831 |
[ |
RPP2A | Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... | 2 | 4 | 31.1% | 0.00293 | 10746 |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 4 | 4 | 20.7% | 8.55E-4 | 42271 |
[ |
YHM2 | Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing n... | 2 | 4 | 13.4% | 9.88E-4 | 34185 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 3 | 4 | 11.8% | 6.21E-4 | 54545 |
[ |
NOP56 | Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that ... | 3 | 4 | 11.3% | 6.16E-4 | 56864 |
[ |
NUG1 | GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits... | 4 | 4 | 10.8% | 5.97E-4 | 57709 |
[ |
SSZ1 | Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that ... | 3 | 4 | 5.9% | 5.77E-4 | 58238 |
[ |
ARP7 | Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved... | 3 | 3 | 13.0% | 4.88E-4 | 53810 |
[ |
SVP26 | Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II... | 3 | 3 | 9.6% | 0.00102 | 26284 |
[ |
VMA2 | Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... | 3 | 3 | 9.1% | 4.5E-4 | 57749 |
[ |
RTT106 | Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced ch... | 2 | 3 | 5.9% | 5.11E-4 | 51621 |
[ |
YGR130C | Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; possible compon... | 3 | 3 | 4.7% | 2.85E-4 | 92698 |
[ |
PDR1 | Zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins t... | 2 | 3 | 3.7% | 2.18E-4 | 121793 |
[ |
GSC2 | Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore... | 3 | 3 | 3.2% | 1.23E-4 | 216988 |
[ |
YJL113W | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 3 | 2.4% | 1.29E-4 | 207708 |
[ |
YHL009W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 3 | 2.4% | 1.29E-4 | 207969 |
[ |
KEM1 | Evolutionarily-conserved 5'-3' exonuclease component of cytoplasmic processing (P) bodies involved i... | 2 | 3 | 2.4% | 1.52E-4 | 175459 |
[ |
ARF1 | ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated vesicle form... | 2 | 2 | 19.3% | 8.57E-4 | 20529 |
[ |
YNL208W | Protein of unknown function; may interact with ribosomes, based on co-purification experiments; auth... | 2 | 2 | 16.7% | 7.6E-4 | 21964 |
[ |
TFS1 | Protein that interacts with and inhibits carboxypeptidase Y and Ira2p; phosphatidylethanolamine-bind... | 2 | 2 | 15.1% | 7.08E-4 | 24357 |
[ |
STP22 | Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into ... | 2 | 2 | 14.5% | 5.24E-4 | 33304 |
[ |
ADH1 | Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... | 2 | 2 | 13.2% | 4.46E-4 | 36849 |
[ |
RIM20 | Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix fam... | 2 | 2 | 7.6% | 2.35E-4 | 75947 |
[ |
RVB2 | Essential protein involved in transcription regulation; component of chromatin remodeling complexes;... | 2 | 2 | 7.2% | 3.29E-4 | 51612 |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 2 | 2 | 5.4% | 2.27E-4 | 74468 |
[ |
HAL5 | Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disrupt... | 2 | 2 | 5.3% | 1.81E-4 | 95455 |
[ |
GCN20 | Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate G... | 2 | 2 | 4.4% | 2.06E-4 | 85027 |
[ |
HIR2 | Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene tra... | 2 | 2 | 4.3% | 1.77E-4 | 98445 |
[ |
SBE22 | Protein involved in the transport of cell wall components from the Golgi to the cell surface; simila... | 2 | 2 | 3.8% | 1.82E-4 | 96398 |
[ |
RGC1 | Pleckstrin homology domain containing protein proposed to function as a glycerol channel activator; ... | 2 | 2 | 3.7% | 1.43E-4 | 120395 |
[ |
SPT5 | Protein involved in regulating Pol I and Pol II transcription and pre-mRNA processing; forms a compl... | 2 | 2 | 3.3% | 1.46E-4 | 115650 |
[ |
COG4 | Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tet... | 2 | 2 | 2.8% | 1.8E-4 | 98665 |
[ |
YPL216W | Putative protein of unknown function; YPL216W is not an essential gene | 2 | 2 | 2.6% | 1.41E-4 | 128133 |
[ |
SNF2 | Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation... | 2 | 2 | 2.5% | 9.11E-5 | 194050 |
[ |
NUP159 | Nucleoporin, subunit of the nuclear pore complex that is found exclusively on the cytoplasmic side, ... | 2 | 2 | 2.5% | 1.06E-4 | 158907 |
[ |
SLH1 | Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; req... | 2 | 2 | 2.3% | 7.89E-5 | 224828 |
[ |
OXP1 | 5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green... | 2 | 2 | 2.3% | 1.21E-4 | 140427 |
[ |
AUS1 | Transporter of the ATP-binding cassette family, involved in uptake of sterols and anaerobic growth | 2 | 2 | 2.0% | 1.11E-4 | 157750 |
[ |
PDR5 | Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter actively regulated by ... | 2 | 2 | 1.9% | 1.03E-4 | 170437 |
[ |
ACC1 | Acetyl-CoA carboxylase, biotin containing enzyme that catalyzes the carboxylation of acetyl-CoA to f... | 2 | 2 | 1.6% | 6.95E-5 | 250351 |
[ |
ARO1 | Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which i... | 2 | 2 | 1.4% | 9.77E-5 | 174754 |