Bait Protein: | VAM3 |
Organism: | Saccharomyces cerevisiae |
Publication: | Hao Xu , Youngsoo Jun, James Thompson, John Yates and William Wickner (2010) HOPS Prevents the Disassembly of Trans-SNARE Complexes by Sec17p/Sec18p during Membrane Fusion [In Press] |
Comments: | sample: hx4 |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
RPP2A | Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... | 4 | 5 | 48.1% | 0.019 | 10746 |
[ |
RPP2B | Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... | 4 | 6 | 36.4% | 0.022 | 11050 |
[ |
TSA1 | Thioredoxin peroxidase, acts as both a ribosome-associated and free cytoplasmic antioxidant; self-as... | 2 | 3 | 12.8% | 0.00617 | 21590 |
[ |
YKT6 | Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and wit... | 2 | 2 | 11.0% | 0.00403 | 22707 |
[ |
VTI1 | Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and ... | 5 | 15 | 32.3% | 0.0279 | 24668 |
[ |
VAM3 | Syntaxin-related protein required for vacuolar assembly; functions with Vam7p in vacuolar protein tr... | 10 | 36 | 36.7% | 0.0513 | 32498 |
[ |
SEC17 | Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' ste... | 10 | 21 | 34.9% | 0.029 | 32803 |
[ |
RPP0 | Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphoryl... | 3 | 5 | 7.4% | 0.00646 | 33717 |
[ |
VAM7 | Component of the vacuole SNARE complex involved in vacuolar morphogenesis; SNAP-25 homolog; function... | 8 | 14 | 23.4% | 0.0179 | 36711 |
[ |
PIL1 | Primary component of eisosomes, which are large immobile cell cortex structures associated with endo... | 2 | 2 | 8.0% | 0.00238 | 38349 |
[ |
VMA6 | Subunit d of the five-subunit V0 integral membrane domain of vacuolar H+-ATPase (V-ATPase), an elect... | 2 | 2 | 11.6% | 0.00234 | 39791 |
[ |
PDB1 | E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved mu... | 3 | 4 | 12.3% | 0.0044 | 40054 |
[ |
CIC1 | Essential protein that interacts with proteasome components and has a potential role in proteasome s... | 2 | 2 | 9.0% | 0.00214 | 42530 |
[ |
UBI4 | Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-... | 2 | 15 | 6.6% | 0.0159 | 42826 |
[ |
IVY1 | Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the... | 4 | 6 | 15.0% | 0.00534 | 49976 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 3 | 3 | 5.7% | 0.00264 | 50033 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 2 | 2 | 6.4% | 0.00161 | 54545 |
[ |
PHO8 | Repressible alkaline phosphatase, a glycoprotein localized to the vacuole; regulated by levels of in... | 3 | 7 | 8.0% | 0.00498 | 63004 |
[ |
PHO84 | High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated... | 2 | 2 | 4.9% | 0.00137 | 64382 |
[ |
CPS1 | Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions | 3 | 3 | 9.5% | 0.0021 | 64597 |
[ |
LYP1 | Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of c... | 2 | 3 | 4.4% | 0.00198 | 68090 |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 4 | 7 | 9.1% | 0.00414 | 74468 |
[ |
FAA4 | Long chain fatty acyl-CoA synthetase, activates imported fatty acids with a preference for C12:0-C16... | 2 | 2 | 4.0% | 0.00116 | 77267 |
[ |
PPN1 | Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer | 3 | 3 | 5.5% | 0.00179 | 78344 |
[ |
SEC18 | ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuol... | 3 | 3 | 6.6% | 0.00159 | 84056 |
[ |
FRA1 | Protein involved in negative regulation of transcription of iron regulon; forms an iron independent ... | 3 | 3 | 6.3% | 0.00161 | 84924 |
[ |
VPH1 | Subunit a of vacuolar-ATPase V0 domain, one of two isoforms (Vph1p and Stv1p); Vph1p is located in V... | 4 | 5 | 7.1% | 0.0024 | 95529 |
[ |
VTC3 | Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuol... | 2 | 2 | 2.9% | 9.65E-4 | 96553 |
[ |
YDR089W | Protein of unknown function; deletion confers resistance to Nickel | 2 | 2 | 3.3% | 9.27E-4 | 98712 |
[ |
PHO91 | Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90,... | 2 | 2 | 2.7% | 9.01E-4 | 99490 |
[ |
PEP3 | Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular ... | 3 | 3 | 4.2% | 0.00132 | 107398 |
[ |
VAM6 | Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by fa... | 2 | 2 | 3.1% | 7.68E-4 | 122881 |
[ |
YPK9 | Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-t... | 4 | 5 | 3.2% | 0.00137 | 166749 |
[ |
YCF1 | Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detox... | 3 | 5 | 3.0% | 0.00133 | 171120 |
[ |
YDR261C-D | Retrotransposon TYA Gag and TYB Pol genes; in YDRCTY1-3 TYB is mutant and probably non-functional | 3 | 4 | 2.7% | 0.001 | 181660 |
[ |
YDR316W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 4 | 2.5% | 9.18E-4 | 198526 |
[ |
YER138C | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 4 | 2.5% | 9.18E-4 | 198561 |
[ |
YER160C | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 4 | 2.5% | 9.18E-4 | 198568 |
[ |
YDR098C-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 4 | 2.5% | 9.18E-4 | 198594 |
[ |
YDR365W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 4 | 2.5% | 9.18E-4 | 198658 |
[ |
YDR210C-D | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 4 | 2.5% | 9.18E-4 | 198838 |
[ |
YBR012W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 4 | 2.5% | 9.18E-4 | 198868 |
[ |
FKS1 | Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... | 4 | 5 | 3.9% | 0.00107 | 214849 |