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Bait Protein: MLP2
Organism: Saccharomyces cerevisiae
Publication: Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561.
Comments: #06 Alpha Factor Prep3-TiO2 Phosphopeptide enrichment

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FILTERED Run Results: [unfilter]

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   YIL002W-A Putative protein of unknown function; identified by expression profiling and mass spectrometry 2 15 60.9% 0.087 7729
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 22 94 45.5% 0.104 42271
[View Peptides]   SPC29 Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... 17 80 32.8% 0.126 29280
[View Peptides]   MLP2 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 31 143 28.2% 0.0341 195140
[View Peptides]   CNM67 Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... 16 31 27.2% 0.0213 67400
[View Peptides]   RPN13 Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for t... 3 10 20.5% 0.0256 17902
[View Peptides]   BBP1 Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... 7 16 19.0% 0.0166 45384
[View Peptides]   BGL2 Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance 7 13 18.5% 0.0166 34119
[View Peptides]   NUD1 Component of the spindle pole body outer plaque, required for exit from mitosis 22 47 15.2% 0.0221 94104
[View Peptides]   NBP1 Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... 2 3 14.1% 0.00376 37354
[View Peptides]   SPC110 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... 15 23 13.7% 0.00975 111782
[View Peptides]   BFA1 Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... 5 8 13.6% 0.00558 66087
[View Peptides]   CDC14 Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR ... 2 5 12.3% 0.00363 61907
[View Peptides]   TUB4 Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of th... 2 14 9.7% 0.0118 52628
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 2 4 9.0% 0.0032 54545
[View Peptides]   ACT1 Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... 2 12 7.5% 0.0128 41690
[View Peptides]   FUS3 Mitogen-activated serine/threonine protein kinase involved in mating; phosphoactivated by Ste7p; sub... 5 21 7.4% 0.0238 40772
[View Peptides]   RRB1 Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with ... 2 6 7.2% 0.0047 57261
[View Peptides]   NOP58 Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the ... 2 16 7.0% 0.0125 56956
[View Peptides]   NPL3 RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from n... 3 7 7.0% 0.00676 45407
[View Peptides]   PSH1 Nuclear protein, putative RNA polymerase II elongation factor; isolated as Pob3p/Spt16p-binding prot... 2 16 6.7% 0.0158 47068
[View Peptides]   PXR1 Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2... 2 6 6.3% 0.00886 31312
[View Peptides]   GAS3 Putative 1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall 2 3 5.5% 0.00229 56794
[View Peptides]   PGK1 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... 2 18 5.3% 0.0173 44738
[View Peptides]   VMA2 Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... 2 7 5.2% 0.00542 57749
[View Peptides]   YEF3 Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes b... 4 10 4.7% 0.00383 115945
[View Peptides]   ACF2 Intracellular beta-1,3-endoglucanase, expression is induced during sporulation; may have a role in c... 2 5 4.6% 0.00257 88403
[View Peptides]   DEF1 RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and prot... 2 24 4.2% 0.013 83973
[View Peptides]   GFA1 Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... 2 4 4.2% 0.00223 80047
[View Peptides]   BDF1 Protein involved in transcription initiation at TATA-containing promoters; associates with the basal... 2 7 3.8% 0.00408 76978
[View Peptides]   HSL7 Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization ... 2 4 3.3% 0.00193 95153
[View Peptides]   BMS1 GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites... 2 8 3.0% 0.00271 135571
[View Peptides]   YAR009C Retrotransposon TYA Gag and TYB Pol genes; in YARCTY1-1 TYB is mutant and probably non-functional 2 5 2.6% 0.00167 136732
[View Peptides]   AFR1 Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture d... 2 3 2.6% 0.00194 71364
[View Peptides]   KRI1 Essential nucleolar protein required for 40S ribosome biogenesis; physically and functionally intera... 3 14 2.4% 0.00948 68654
[View Peptides]   YDR261C-D Retrotransposon TYA Gag and TYB Pol genes; in YDRCTY1-3 TYB is mutant and probably non-functional 2 5 1.9% 0.00125 181660
[View Peptides]   YJR027W Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198651
[View Peptides]   YDR316W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198526
[View Peptides]   YDR210C-D Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198838
[View Peptides]   YLR157C-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198544
[View Peptides]   YDR098C-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198594
[View Peptides]   YDR365W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198658
[View Peptides]   YER138C Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198561
[View Peptides]   YML039W Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198571
[View Peptides]   YGR038C-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198520
[View Peptides]   YLR227W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198404
[View Peptides]   YJR029W Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198616
[View Peptides]   YML045W Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198524
[View Peptides]   YGR027W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198656

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