Bait Protein: | MLP2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561. |
Comments: | #30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
YIL002W-A | Putative protein of unknown function; identified by expression profiling and mass spectrometry | 26 | 27 | 92.8% | 0.165 | 7729 |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 64 | 74 | 91.7% | 0.086 | 42271 |
[ |
SPC29 | Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... | 42 | 43 | 88.9% | 0.0717 | 29280 |
[ |
CMD1 | Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... | 12 | 13 | 85.0% | 0.0373 | 16135 |
[ |
MLP2 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 245 | 285 | 84.5% | 0.0716 | 195140 |
[ |
SPC110 | Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... | 126 | 144 | 77.6% | 0.0644 | 111782 |
[ |
CDC31 | Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... | 15 | 16 | 75.2% | 0.0419 | 18751 |
[ |
CNM67 | Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... | 71 | 79 | 69.7% | 0.0574 | 67400 |
[ |
BFA1 | Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... | 21 | 24 | 49.3% | 0.0176 | 66087 |
[ |
NUD1 | Component of the spindle pole body outer plaque, required for exit from mitosis | 39 | 41 | 35.3% | 0.0203 | 94104 |
[ |
NBP1 | Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... | 6 | 6 | 27.9% | 0.00794 | 37354 |
[ |
BBP1 | Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... | 7 | 7 | 18.7% | 0.00767 | 45384 |
[ |
CDC14 | Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR ... | 6 | 6 | 18.1% | 0.00459 | 61907 |
[ |
YML131W | Putative protein of unknown function with similarity to medium chain dehydrogenase/reductases; expre... | 2 | 2 | 18.1% | 0.00231 | 39976 |
[ |
MPS2 | Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... | 3 | 3 | 13.4% | 0.00327 | 44585 |
[ |
STB3 | Ribosomal RNA processing element (RRPE)-binding protein involved in the induction of non-ribosomal p... | 2 | 2 | 12.9% | 0.00164 | 56264 |
[ |
TUB4 | Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of th... | 2 | 2 | 12.3% | 0.00178 | 52628 |
[ |
SPC72 | Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... | 5 | 5 | 11.9% | 0.00339 | 72105 |
[ |
UTR1 | ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of fer... | 2 | 2 | 9.6% | 0.00159 | 59469 |
[ |
BUB2 | Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spi... | 2 | 2 | 9.5% | 0.00276 | 35028 |
[ |
MLP1 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 9 | 9 | 6.7% | 0.00203 | 218454 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 2 | 2 | 6.3% | 9.19E-4 | 99619 |
[ |
MLH1 | Protein required for mismatch repair in mitosis and meiosis as well as crossing over during meiosis;... | 2 | 2 | 6.1% | 0.0011 | 87062 |
[ |
PPZ2 | Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transp... | 2 | 2 | 5.9% | 0.00119 | 78492 |
[ |
ACS2 | Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histo... | 2 | 2 | 5.9% | 0.00124 | 75492 |
[ |
PUF3 | Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anter... | 2 | 2 | 5.8% | 9.6E-4 | 98068 |
[ |
MIP1 | Catalytic subunit of the mitochondrial DNA polymerase; conserved C-terminal segment is required for ... | 3 | 3 | 5.5% | 0.00101 | 143502 |
[ |
URC2 | Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a scree... | 2 | 2 | 4.9% | 0.00109 | 88648 |
[ |
DIS3 | Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; ... | 2 | 2 | 4.8% | 8.43E-4 | 113707 |
[ |
VAC8 | Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for ... | 2 | 2 | 4.8% | 0.00146 | 63208 |
[ |
VTH1 | Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in ... | 3 | 3 | 4.7% | 8.17E-4 | 174427 |
[ |
VTH2 | Putative membrane glycoprotein with strong similarity to Vth1p and Pep1p/Vps10p, may be involved in ... | 3 | 3 | 4.7% | 8.17E-4 | 174401 |
[ |
SPT21 | Protein required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not... | 2 | 2 | 4.7% | 0.00111 | 84697 |
[ |
HSL1 | Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates wit... | 3 | 3 | 4.3% | 8.34E-4 | 169592 |
[ |
KAP114 | Karyopherin, responsible for nuclear import of Spt15p, histones H2A and H2B, and Nap1p; amino termin... | 2 | 2 | 4.2% | 8.4E-4 | 113921 |
[ |
YJL113W | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 3 | 3.9% | 7.02E-4 | 207708 |
[ |
MPH1 | Member of the DEAH family of helicases, functions in an error-free DNA damage bypass pathway that in... | 2 | 2 | 3.8% | 8.5E-4 | 114058 |
[ |
SLN1 | Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intra... | 3 | 3 | 3.7% | 0.00104 | 134435 |
[ |
NDC1 | Nuclear envelope protein with multiple putative transmembrane domains, required for nuclear pore com... | 2 | 2 | 3.7% | 0.00129 | 74134 |
[ |
SPC97 | Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... | 2 | 2 | 3.5% | 0.00103 | 96825 |
[ |
HSC82 | Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... | 2 | 2 | 3.5% | 0.0012 | 80900 |
[ |
FAS2 | Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty ... | 3 | 3 | 3.4% | 6.71E-4 | 206945 |
[ |
SFI1 | Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the ... | 3 | 3 | 3.4% | 0.00134 | 112978 |
[ |
TUS1 | Guanine nucleotide exchange factor (GEF) that functions to modulate Rho1p activity as part of the ce... | 3 | 3 | 3.1% | 9.68E-4 | 149112 |
[ |
UBP7 | Ubiquitin-specific protease that cleaves ubiquitin-protein fusions | 2 | 2 | 3.1% | 7.88E-4 | 123134 |
[ |
BOI2 | Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence ... | 2 | 2 | 2.8% | 8.11E-4 | 115688 |
[ |
AIM21 | Protein of unknown function involved in mitochondrial migration along actin filament; may interact w... | 2 | 2 | 2.8% | 0.00124 | 74763 |
[ |
GIP3 | Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromo... | 2 | 2 | 2.7% | 6.61E-4 | 140891 |
[ |
FUN30 | Protein whose overexpression affects chromosome stability, potential Cdc28p substrate; homolog of Sn... | 2 | 2 | 2.7% | 7.46E-4 | 128507 |
[ |
SPC98 | Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... | 2 | 2 | 2.4% | 9.97E-4 | 98227 |
[ |
SMC4 | Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with... | 2 | 2 | 2.3% | 5.95E-4 | 162188 |
[ |
TRK1 | Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter... | 2 | 2 | 2.3% | 6.83E-4 | 141073 |
[ |
RAT1 | Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snR... | 2 | 2 | 2.3% | 8.39E-4 | 115934 |
[ |
SMC1 | Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation an... | 2 | 2 | 2.1% | 6.89E-4 | 141280 |
[ |
TCB2 | Bud-specific protein with a potential role in membrane trafficking; GFP-fusion protein migrates from... | 2 | 2 | 2.0% | 7.16E-4 | 132509 |
[ |
UTP20 | Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA | 2 | 2 | 1.9% | 3.38E-4 | 287559 |
[ |
BEM2 | Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cell... | 3 | 3 | 1.8% | 5.84E-4 | 245428 |
[ |
YLR422W | Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange fact... | 2 | 2 | 1.8% | 4.37E-4 | 221562 |
[ |
SMC3 | Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells;... | 2 | 2 | 1.7% | 6.86E-4 | 141336 |
[ |
RPO41 | Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; re... | 2 | 2 | 1.5% | 6.25E-4 | 153047 |
[ |
STT4 | Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for norma... | 2 | 2 | 1.2% | 4.44E-4 | 214605 |
[ |
CDC25 | Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates ... | 2 | 2 | 1.1% | 5.31E-4 | 179091 |
[ |
RAV1 | Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzym... | 2 | 2 | 1.1% | 6.22E-4 | 154932 |
[ |
MDN1 | Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex... | 2 | 2 | 0.7% | 1.72E-4 | 559316 |