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Bait Protein: MLP2
Organism: Saccharomyces cerevisiae
Publication: Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561.
Comments: #10 Mitotic Prep1-TiO2 Phosphopeptide enrichment

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FILTERED Run Results: [unfilter]

[View our MS interpretation guidelines.]
  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   YIL002W-A Putative protein of unknown function; identified by expression profiling and mass spectrometry 20 26 94.2% 0.142 7729
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 52 60 79.1% 0.0624 42271
[View Peptides]   CDC31 Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... 12 13 75.2% 0.0305 18751
[View Peptides]   MLP2 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 235 296 74.7% 0.0666 195140
[View Peptides]   CMD1 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... 12 13 74.1% 0.0334 16135
[View Peptides]   SPC110 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... 135 161 74.0% 0.0644 111782
[View Peptides]   CNM67 Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... 50 61 66.3% 0.0396 67400
[View Peptides]   SPC72 Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... 26 27 59.0% 0.0164 72105
[View Peptides]   RPP1B Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of tra... 2 2 56.6% 0.00712 10668
[View Peptides]   RPP1A Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation facto... 2 2 55.7% 0.00712 10908
[View Peptides]   SPC29 Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... 14 26 54.9% 0.0388 29280
[View Peptides]   TUB4 Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of th... 11 12 44.8% 0.00958 52628
[View Peptides]   RPP2B Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... 2 2 43.6% 0.00687 11050
[View Peptides]   BFA1 Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... 17 17 39.5% 0.0112 66087
[View Peptides]   BBP1 Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... 10 10 38.4% 0.00981 45384
[View Peptides]   NBP1 Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... 10 10 38.2% 0.0118 37354
[View Peptides]   YLR146W-A Putative protein of unknown function 2 2 35.5% 0.0122 7078
[View Peptides]   RPP2A Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... 3 3 34.9% 0.0107 10746
[View Peptides]   NUD1 Component of the spindle pole body outer plaque, required for exit from mitosis 34 36 31.6% 0.016 94104
[View Peptides]   STM1 Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with r... 3 3 23.4% 0.00415 29995
[View Peptides]   KAR1 Essential protein involved in karyogamy during mating and in spindle pole body duplication during mi... 7 7 20.8% 0.0061 50653
[View Peptides]   MPS2 Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... 4 4 18.3% 0.0039 44585
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 8 8 13.6% 0.00329 99619
[View Peptides]   PDC1 Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... 3 3 13.5% 0.00201 61495
[View Peptides]   HSC82 Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... 4 4 11.5% 0.00214 80900
[View Peptides]   SUP35 Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a domi... 4 4 10.8% 0.00221 76551
[View Peptides]   SPC98 Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... 4 5 9.9% 0.00223 98227
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 3 3 9.8% 0.00227 54545
[View Peptides]   SPC97 Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... 6 7 9.4% 0.00321 96825
[View Peptides]   TCP1 Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; inv... 3 3 8.1% 0.00203 60481
[View Peptides]   PSH1 Nuclear protein, putative RNA polymerase II elongation factor; isolated as Pob3p/Spt16p-binding prot... 2 2 8.1% 0.00186 47068
[View Peptides]   ASK10 Component of the RNA polymerase II holoenzyme, phosphorylated in response to oxidative stress; has a... 4 4 7.4% 0.00132 126864
[View Peptides]   SPB1 AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation;... 3 3 7.4% 0.00135 96485
[View Peptides]   CDC5 Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and ... 3 3 7.0% 0.00161 81031
[View Peptides]   RIX7 Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucl... 2 2 6.2% 9.02E-4 93069
[View Peptides]   MPP10 Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts... 2 2 6.2% 0.00127 66953
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 2 2 5.7% 0.00111 74468
[View Peptides]   POM152 Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore... 4 4 5.5% 0.00113 151652
[View Peptides]   MLP1 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 5 5 4.7% 0.00101 218454
[View Peptides]   HSL7 Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization ... 2 4 4.5% 0.00183 95153
[View Peptides]   UBI4 Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-... 2 3 3.9% 0.00297 42826
[View Peptides]   EFT2, EFT1 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... 2 2 3.9% 8.97E-4 93289
[View Peptides]   SFI1 Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the ... 4 4 3.7% 0.0016 112978
[View Peptides]   GAT1 Transcriptional activator of genes involved in nitrogen catabolite repression; contains a GATA-1-typ... 2 2 3.7% 0.00148 56328
[View Peptides]   STU2 Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics duri... 2 2 3.2% 8.5E-4 100918
[View Peptides]   ACS2 Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histo... 2 2 3.1% 0.00111 75492
[View Peptides]   FKS1 Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... 2 2 2.1% 4.03E-4 214849
YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle