Bait Protein: | MLP2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561. |
Comments: | #13 Mitotic Prep2-TiO2 Phosphopeptide enrichment |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
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YIL002W-A | Putative protein of unknown function; identified by expression profiling and mass spectrometry | 20 | 22 | 98.6% | 0.148 | 7729 |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 61 | 92 | 87.1% | 0.117 | 42271 |
[ |
CNM67 | Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... | 68 | 92 | 76.2% | 0.0733 | 67400 |
[ |
MLP2 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 165 | 213 | 75.8% | 0.0587 | 195140 |
[ |
YLR146W-A | Putative protein of unknown function | 2 | 2 | 72.6% | 0.0149 | 7078 |
[ |
RPP1B | Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of tra... | 2 | 2 | 71.7% | 0.00873 | 10668 |
[ |
NUD1 | Component of the spindle pole body outer plaque, required for exit from mitosis | 64 | 79 | 68.3% | 0.0429 | 94104 |
[ |
SPC110 | Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... | 59 | 63 | 64.3% | 0.0309 | 111782 |
[ |
TUB4 | Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of th... | 13 | 15 | 60.0% | 0.0147 | 52628 |
[ |
SPC29 | Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... | 24 | 35 | 54.5% | 0.064 | 29280 |
[ |
CMD1 | Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... | 6 | 30 | 52.4% | 0.0944 | 16135 |
[ |
SPC72 | Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... | 29 | 36 | 49.4% | 0.0268 | 72105 |
[ |
CDC31 | Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... | 6 | 8 | 47.8% | 0.023 | 18751 |
[ |
BFA1 | Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... | 10 | 11 | 27.7% | 0.00887 | 66087 |
[ |
BBP1 | Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... | 7 | 8 | 24.9% | 0.00961 | 45384 |
[ |
CDC14 | Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR ... | 6 | 9 | 22.0% | 0.00756 | 61907 |
[ |
NBP1 | Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... | 3 | 3 | 17.9% | 0.00435 | 37354 |
[ |
COQ4 | Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization o... | 2 | 2 | 16.4% | 0.00276 | 38627 |
[ |
BUB2 | Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spi... | 4 | 4 | 15.4% | 0.00605 | 35028 |
[ |
SPC97 | Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... | 7 | 7 | 13.5% | 0.00393 | 96825 |
[ |
CDC5 | Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and ... | 5 | 5 | 12.5% | 0.00328 | 81031 |
[ |
MPS2 | Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... | 2 | 4 | 10.1% | 0.00478 | 44585 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 2 | 2 | 8.4% | 0.00185 | 54545 |
[ |
PDC1 | Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... | 2 | 2 | 7.3% | 0.00164 | 61495 |
[ |
SPC98 | Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... | 2 | 2 | 5.7% | 0.00109 | 98227 |
[ |
SFI1 | Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the ... | 4 | 4 | 3.7% | 0.00196 | 112978 |
[ |
KOG1 | Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or To... | 2 | 2 | 1.7% | 5.94E-4 | 177608 |
[ |
TRA1 | Subunit of SAGA and NuA4 histone acetyltransferase complexes; interacts with acidic activators (e.g.... | 2 | 2 | 1.1% | 2.47E-4 | 433182 |