Bait Protein: | MLP2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561. |
Comments: | #26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2 |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
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YIL002W-A | Putative protein of unknown function; identified by expression profiling and mass spectrometry | 10 | 11 | 98.6% | 0.0442 | 7729 |
[ |
CDC31 | Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... | 22 | 36 | 95.7% | 0.0619 | 18751 |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 104 | 210 | 92.8% | 0.16 | 42271 |
[ |
SPC29 | Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... | 51 | 98 | 88.5% | 0.107 | 29280 |
[ |
CMD1 | Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... | 9 | 18 | 85.7% | 0.0339 | 16135 |
[ |
SPC110 | Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... | 199 | 377 | 83.3% | 0.111 | 111782 |
[ |
BFA1 | Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... | 47 | 72 | 67.2% | 0.0347 | 66087 |
[ |
CNM67 | Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... | 74 | 122 | 65.1% | 0.0582 | 67400 |
[ |
NBP1 | Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... | 12 | 12 | 46.7% | 0.0104 | 37354 |
[ |
MLP2 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 83 | 115 | 45.3% | 0.019 | 195140 |
[ |
BBP1 | Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... | 14 | 20 | 44.4% | 0.0144 | 45384 |
[ |
NUD1 | Component of the spindle pole body outer plaque, required for exit from mitosis | 62 | 117 | 41.2% | 0.0381 | 94104 |
[ |
CDC14 | Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR ... | 19 | 28 | 35.8% | 0.0141 | 61907 |
[ |
MPS2 | Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... | 12 | 15 | 31.0% | 0.0107 | 44585 |
[ |
KAR1 | Essential protein involved in karyogamy during mating and in spindle pole body duplication during mi... | 11 | 15 | 23.8% | 0.00959 | 50653 |
[ |
SPC72 | Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... | 8 | 8 | 16.1% | 0.00356 | 72105 |
[ |
ENO2 | Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... | 4 | 4 | 14.2% | 0.00253 | 46914 |
[ |
FBA1 | Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversio... | 2 | 3 | 13.1% | 0.00231 | 39621 |
[ |
RSM28 | Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role i... | 2 | 2 | 12.7% | 0.00153 | 41216 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 6 | 6 | 12.6% | 0.00332 | 54545 |
[ |
SFI1 | Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the ... | 9 | 10 | 9.3% | 0.00293 | 112978 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 7 | 8 | 8.6% | 0.00241 | 99619 |
[ |
GYP1 | Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, ... | 2 | 2 | 8.5% | 8.7E-4 | 73289 |
[ |
PMA2 | Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator ... | 7 | 8 | 8.3% | 0.00234 | 102172 |
[ |
PIR3 | O-glycosylated covalently-bound cell wall protein required for cell wall stability; expression is ce... | 2 | 3 | 8.3% | 0.00256 | 33004 |
[ |
CIS3 | Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with inter... | 2 | 3 | 7.9% | 0.00366 | 23242 |
[ |
NOP1 | Nucleolar protein, component of the small subunit processome complex, which is required for processi... | 4 | 4 | 7.6% | 0.00339 | 34465 |
[ |
NOP58 | Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the ... | 3 | 3 | 7.0% | 0.00163 | 56956 |
[ |
MPP10 | Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts... | 2 | 2 | 6.4% | 9.34E-4 | 66953 |
[ |
DED81 | Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attach... | 2 | 4 | 5.6% | 0.002 | 62207 |
[ |
PIR1 | O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-gluc... | 2 | 3 | 5.3% | 0.00244 | 34622 |
[ |
HSP150 | O-mannosylated heat shock protein that is secreted and covalently attached to the cell wall via beta... | 2 | 3 | 4.7% | 0.00215 | 38619 |
[ |
MLP1 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 5 | 5 | 4.2% | 7.39E-4 | 218454 |
[ |
HSL7 | Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization ... | 3 | 4 | 3.3% | 0.00134 | 95153 |
[ |
MYO1 | Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the... | 2 | 2 | 2.7% | 2.87E-4 | 223634 |
[ |
CHD1 | Nucleosome remodeling factor that functions in regulation of transcription elongation; contains a ch... | 2 | 2 | 2.5% | 3.77E-4 | 168240 |