Bait Protein: | MLP2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561. |
Comments: | #06 Alpha Factor Prep3-TiO2 Phosphopeptide enrichment |
Raw Data: |
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UNFILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
MLP2 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 31 | 143 | 28.2% | 0.0341 | 195140 |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 22 | 94 | 45.5% | 0.104 | 42271 |
[ |
SPC29 | Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... | 17 | 80 | 32.8% | 0.126 | 29280 |
[ |
NUD1 | Component of the spindle pole body outer plaque, required for exit from mitosis | 22 | 47 | 15.2% | 0.0221 | 94104 |
[ |
CNM67 | Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... | 16 | 31 | 27.2% | 0.0213 | 67400 |
[ |
DEF1 | RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and prot... | 2 | 24 | 4.2% | 0.013 | 83973 |
[ |
SPC110 | Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... | 15 | 23 | 13.7% | 0.00975 | 111782 |
[ |
FUS3 | Mitogen-activated serine/threonine protein kinase involved in mating; phosphoactivated by Ste7p; sub... | 5 | 21 | 7.4% | 0.0238 | 40772 |
[ |
PGK1 | 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... | 2 | 18 | 5.3% | 0.0173 | 44738 |
[ |
RPS3 | Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease ... | 3 | 17 | 16.7% | 0.0283 | 26503 |
[ |
BBP1 | Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... | 7 | 16 | 19.0% | 0.0166 | 45384 |
[ |
NOP58 | Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the ... | 2 | 16 | 7.0% | 0.0125 | 56956 |
[ |
PSH1 | Nuclear protein, putative RNA polymerase II elongation factor; isolated as Pob3p/Spt16p-binding prot... | 2 | 16 | 6.7% | 0.0158 | 47068 |
[ |
YIL002W-A | Putative protein of unknown function; identified by expression profiling and mass spectrometry | 2 | 15 | 60.9% | 0.087 | 7729 |
[ |
TUB4 | Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of th... | 2 | 14 | 9.7% | 0.0118 | 52628 |
[ |
PRT1 | eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translati... | 1 | 14 | 4.2% | 0.00734 | 88130 |
[ |
KRI1 | Essential nucleolar protein required for 40S ribosome biogenesis; physically and functionally intera... | 3 | 14 | 2.4% | 0.00948 | 68654 |
[ |
BGL2 | Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance | 7 | 13 | 18.5% | 0.0166 | 34119 |
[ |
ACT1 | Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... | 2 | 12 | 7.5% | 0.0128 | 41690 |
[ |
DIG1 | Regulatory protein of unknown function, constitutively-expressed, involved in the regulation of mati... | 1 | 12 | 3.8% | 0.0106 | 49356 |
[ |
RPP2B | Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... | 1 | 11 | 43.6% | 0.04 | 11050 |
[ |
RPN13 | Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for t... | 3 | 10 | 20.5% | 0.0256 | 17902 |
[ |
gi|2119236, gi|4... | sp|P35527|K1CI_HUMAN Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9), gi|545257|gb|AAC60... | 4 | 10 | 10.0% | 0.00643 | 61987 |
[ |
YEF3 | Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes b... | 4 | 10 | 4.7% | 0.00383 | 115945 |
[ |
CMD1 | Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... | 1 | 8 | 25.2% | 0.0218 | 16135 |
[ |
BFA1 | Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... | 5 | 8 | 13.6% | 0.00558 | 66087 |
[ |
BMS1 | GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites... | 2 | 8 | 3.0% | 0.00271 | 135571 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 1 | 8 | 2.8% | 0.00349 | 99619 |
[ |
PMA2 | Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator ... | 1 | 8 | 2.7% | 0.00338 | 102172 |
[ |
RPL13B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Ap; not essential f... | 2 | 7 | 14.6% | 0.0141 | 22525 |
[ |
YOR287C | Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of ... | 1 | 7 | 8.3% | 0.00933 | 36125 |
[ |
YDJ1 | Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein trans... | 1 | 7 | 8.1% | 0.00685 | 44671 |
[ |
NPL3 | RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from n... | 3 | 7 | 7.0% | 0.00676 | 45407 |
[ |
VMA2 | Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... | 2 | 7 | 5.2% | 0.00542 | 57749 |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 1 | 7 | 4.4% | 0.00411 | 74468 |
[ |
BDF1 | Protein involved in transcription initiation at TATA-containing promoters; associates with the basal... | 2 | 7 | 3.8% | 0.00408 | 76978 |
[ |
ACC1 | Acetyl-CoA carboxylase, biotin containing enzyme that catalyzes the carboxylation of acetyl-CoA to f... | 1 | 7 | 0.9% | 0.00125 | 250351 |
[ |
TDH3 | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tet... | 3 | 6 | 14.8% | 0.00723 | 35747 |
[ |
DED1 | ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... | 1 | 6 | 9.9% | 0.00397 | 65553 |
[ |
RPS19B | Protein component of the small (40S) ribosomal subunit, required for assembly and maturation of pre-... | 1 | 6 | 9.7% | 0.0167 | 15891 |
[ |
RPS19A | Protein component of the small (40S) ribosomal subunit, required for assembly and maturation of pre-... | 1 | 6 | 9.7% | 0.0167 | 15917 |
[ |
RRB1 | Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with ... | 2 | 6 | 7.2% | 0.0047 | 57261 |
[ |
PXR1 | Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2... | 2 | 6 | 6.3% | 0.00886 | 31312 |
[ |
PDI1 | Protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, es... | 1 | 6 | 5.9% | 0.0046 | 58227 |
[ |
TDH2 | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tet... | 3 | 5 | 14.8% | 0.00602 | 35847 |
[ |
CDC14 | Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR ... | 2 | 5 | 12.3% | 0.00363 | 61907 |
[ |
PIL1 | Primary component of eisosomes, which are large immobile cell cortex structures associated with endo... | 1 | 5 | 8.0% | 0.0059 | 38349 |
[ |
GRS1 | Cytoplasmic and mitochondrial glycyl-tRNA synthase that ligates glycine to the cognate anticodon bea... | 1 | 5 | 4.9% | 0.003 | 75411 |
[ |
ACF2 | Intracellular beta-1,3-endoglucanase, expression is induced during sporulation; may have a role in c... | 2 | 5 | 4.6% | 0.00257 | 88403 |
[ |
PAB1 | Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between th... | 1 | 5 | 4.3% | 0.00347 | 64344 |
[ |
YAR009C | Retrotransposon TYA Gag and TYB Pol genes; in YARCTY1-1 TYB is mutant and probably non-functional | 2 | 5 | 2.6% | 0.00167 | 136732 |
[ |
YDR261C-D | Retrotransposon TYA Gag and TYB Pol genes; in YDRCTY1-3 TYB is mutant and probably non-functional | 2 | 5 | 1.9% | 0.00125 | 181660 |
[ |
YJR027W | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 5 | 1.8% | 0.00114 | 198651 |
[ |
YDR316W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 5 | 1.8% | 0.00114 | 198526 |
[ |
YDR210C-D | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 5 | 1.8% | 0.00114 | 198838 |
[ |
YLR157C-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 5 | 1.8% | 0.00114 | 198544 |
[ |
YDR098C-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 5 | 1.8% | 0.00114 | 198594 |
[ |
YDR365W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 5 | 1.8% | 0.00114 | 198658 |
[ |
YER138C | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 5 | 1.8% | 0.00114 | 198561 |
[ |
YML039W | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 5 | 1.8% | 0.00114 | 198571 |
[ |
YGR038C-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 5 | 1.8% | 0.00114 | 198520 |
[ |
YLR227W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 5 | 1.8% | 0.00114 | 198404 |
[ |
YJR029W | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 5 | 1.8% | 0.00114 | 198616 |
[ |
YML045W | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 5 | 1.8% | 0.00114 | 198524 |
[ |
YGR027W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 2 | 5 | 1.8% | 0.00114 | 198656 |
[ |
NRL_1IKFL, Conta... | owl|| Immunoglobulin igg1-kappa antibody fragment fab complexed With... | 2 | 4 | 15.4% | 0.00748 | 23527 |
[ |
Contaminant_S682... | owl|S68241| immunoglobulin light chain (Mab13-1) - mouse (fragment) | 2 | 4 | 15.1% | 0.00734 | 23846 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 2 | 4 | 9.0% | 0.0032 | 54545 |
[ |
ARB1 | ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has sim... | 1 | 4 | 6.2% | 0.00262 | 68377 |
[ |
GFA1 | Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... | 2 | 4 | 4.2% | 0.00223 | 80047 |
[ |
HSL7 | Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization ... | 2 | 4 | 3.3% | 0.00193 | 95153 |
[ |
TIM11 | Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex re... | 1 | 3 | 34.4% | 0.0125 | 10875 |
[ |
NBP1 | Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... | 2 | 3 | 14.1% | 0.00376 | 37354 |
[ |
RPL22A | Protein component of the large (60S) ribosomal subunit, has similarity to Rpl22Bp and to rat L22 rib... | 1 | 3 | 14.0% | 0.00992 | 13693 |
[ |
RPL12B, RPL12A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b d... | 2 | 3 | 13.9% | 0.00727 | 17823 |
[ |
FPR4 | Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes... | 1 | 3 | 8.7% | 0.00306 | 43903 |
[ |
ASC1 | G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 th... | 1 | 3 | 6.6% | 0.00376 | 34805 |
[ |
GAS3 | Putative 1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall | 2 | 3 | 5.5% | 0.00229 | 56794 |
[ |
SPC72 | Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... | 1 | 3 | 3.7% | 0.00193 | 72105 |
[ |
PDA1 | E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarbo... | 1 | 3 | 3.3% | 0.00286 | 46343 |
[ |
AFR1 | Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture d... | 2 | 3 | 2.6% | 0.00194 | 71364 |
[ |
RPS26B | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps26Ap and has similari... | 1 | 2 | 12.6% | 0.00672 | 13447 |
[ |
RPS26A | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps26Bp and has similari... | 1 | 2 | 12.6% | 0.00672 | 13505 |
[ |
ALD6 | Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; re... | 1 | 2 | 7.6% | 0.0016 | 54414 |
[ |
WTM1 | Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes;... | 1 | 2 | 7.6% | 0.00183 | 48383 |
[ |
ENO1 | Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosph... | 1 | 2 | 3.9% | 0.00183 | 46816 |
[ |
ENO2 | Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... | 1 | 2 | 3.9% | 0.00183 | 46914 |
[ |
CTS1 | Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 ... | 1 | 2 | 2.7% | 0.00142 | 59015 |
[ |
FKS1 | Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... | 1 | 2 | 1.5% | 4.26E-4 | 214849 |
[ |
HTA1 | Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of tw... | 1 | 1 | 25.0% | 0.00303 | 13989 |
[ |
HTA2 | Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of tw... | 1 | 1 | 25.0% | 0.00303 | 13989 |
[ |
GRX5 | Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; mitochondrial ... | 1 | 1 | 16.7% | 0.00267 | 16931 |
[ |
BUD21 | Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated... | 1 | 1 | 8.9% | 0.00187 | 24385 |
[ |
YPR148C | Protein of unknown function that may interact with ribosomes, based on co-purification experiments; ... | 1 | 1 | 7.8% | 9.2E-4 | 49279 |
[ |
RPN8 | Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 prot... | 1 | 1 | 6.2% | 0.00118 | 38313 |
[ |
RPL10 | Protein component of the large (60S) ribosomal subunit, responsible for joining the 40S and 60S subu... | 1 | 1 | 5.9% | 0.00181 | 25361 |
[ |
RPL5 | Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 rib... | 1 | 1 | 5.7% | 0.00135 | 33715 |
[ |
MRH1 | Protein that localizes primarily to the plasma membrane, also found at the nuclear envelope; the aut... | 1 | 1 | 5.6% | 0.00125 | 36191 |
[ |
CCT5 | Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of... | 1 | 1 | 5.3% | 7.12E-4 | 61914 |
[ |
RPL7A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl7Bp and has similarit... | 1 | 1 | 5.3% | 0.00164 | 27638 |
[ |
RPL7B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl7Ap and has similarit... | 1 | 1 | 5.3% | 0.00164 | 27696 |
[ |
RVB1 | Essential protein involved in transcription regulation; component of chromatin remodeling complexes;... | 1 | 1 | 5.2% | 8.64E-4 | 50453 |
[ |
RNR4 | Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiti... | 1 | 1 | 4.1% | 0.00116 | 40055 |
[ |
NOP56 | Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that ... | 1 | 1 | 3.4% | 7.94E-4 | 56864 |
[ |
MPS2 | Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... | 1 | 1 | 3.4% | 0.00103 | 44585 |
[ |
gi|34033, gi|71526 | pir||KRHU3 keratin 13, type I, cytoskeletal, long splice form - human, gi|34033|emb|CAA32786.1| ker... | 1 | 1 | 3.1% | 8.73E-4 | 49644 |
[ |
FBA1 | Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversio... | 1 | 1 | 3.1% | 0.00111 | 39621 |
[ |
LSB3 | Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldric... | 1 | 1 | 2.9% | 8.87E-4 | 48547 |
[ |
NOP1 | Nucleolar protein, component of the small subunit processome complex, which is required for processi... | 1 | 1 | 2.8% | 0.00122 | 34465 |
[ |
SSA2 | ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shoc... | 1 | 1 | 2.7% | 6.26E-4 | 69470 |
[ |
SEC2 | Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle tr... | 1 | 1 | 2.6% | 5.27E-4 | 84652 |
[ |
SSA1 | ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... | 1 | 1 | 2.6% | 6.23E-4 | 69768 |
[ |
Reverse_IPI:IPI0... | null, Ubiquitin-activating enzyme E1 homolog (D8), (NM_003335) ubiquitin-activating enzyme E1-like; ... | 1 | 1 | 2.0% | 3.96E-4 | 111720 |
[ |
SSB1 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 1 | 1 | 2.0% | 6.53E-4 | 66602 |
[ |
SSB2 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 1 | 1 | 2.0% | 6.53E-4 | 66595 |
[ |
Reverse_YBR198C | TAF5 SGDID:S000000402, Chr II from 618518-616122, reverse complement, Verified ORF, "Subunit (90 kDa... | 1 | 1 | 1.9% | 5.01E-4 | 88968 |
[ |
IFH1 | Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promo... | 1 | 1 | 1.8% | 3.69E-4 | 122491 |