Bait Protein: | MLP2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561. |
Comments: | #27 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps3-5 |
Raw Data: |
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UNFILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
CDC31 | Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... | 3 | 8 | 40.4% | 0.252 | 18751 |
[ |
NUD1 | Component of the spindle pole body outer plaque, required for exit from mitosis | 2 | 8 | 2.2% | 0.0477 | 94104 |
[ |
CMD1 | Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... | 2 | 6 | 25.2% | 0.207 | 16135 |
[ |
SPC29 | Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... | 4 | 5 | 15.0% | 0.1 | 29280 |
[ |
BFA1 | Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... | 3 | 4 | 5.2% | 0.0354 | 66087 |
[ |
RPL5 | Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 rib... | 1 | 3 | 10.4% | 0.0512 | 33715 |
[ |
CDC14 | Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR ... | 2 | 2 | 12.7% | 0.0184 | 61907 |
[ |
CNM67 | Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... | 1 | 2 | 5.9% | 0.0175 | 67400 |
[ |
RDL2 | Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, an... | 1 | 1 | 18.8% | 0.034 | 16697 |
[ |
SNP1 | Component of U1 snRNP required for mRNA splicing via spliceosome; may interact with poly(A) polymera... | 1 | 1 | 13.3% | 0.0169 | 34447 |
[ |
FBA1 | Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversio... | 1 | 1 | 12.3% | 0.0141 | 39621 |
[ |
YNR040W | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purifi... | 1 | 1 | 9.4% | 0.0198 | 28636 |
[ |
Reverse_YBR192W | RIM2 SGDID:S000000396, Chr II from 607647-608780, Verified ORF, "Mitochondrial pyrimidine nucleotide... | 1 | 1 | 9.3% | 0.0134 | 42102 |
[ |
MID2 | O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and a... | 1 | 1 | 8.2% | 0.0135 | 39146 |
[ |
YDJ1 | Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein trans... | 1 | 1 | 8.1% | 0.0124 | 44671 |
[ |
Reverse_YKL141W | SDH3 SGDID:S000001624, Chr XI from 179672-180268, Verified ORF, "Cytochrome b subunit of succinate d... | 1 | 1 | 8.1% | 0.0255 | 22068 |
[ |
MPS2 | Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... | 1 | 1 | 7.2% | 0.0131 | 44585 |
[ |
YHB1 | Nitric oxide oxidoreductase, flavohemoglobin involved in nitric oxide detoxification; plays a role i... | 1 | 1 | 5.3% | 0.0127 | 44646 |
[ |
Reverse_YBL097W | BRN1 SGDID:S000000193, Chr II from 40828-43092, Verified ORF, "Essential protein required for chromo... | 1 | 1 | 3.7% | 0.00672 | 86230 |
[ |
SMF1 | Divalent metal ion transporter with a broad specificity for di-valent and tri-valent metals; post-tr... | 1 | 1 | 3.3% | 0.00882 | 63264 |
[ |
PMC1 | Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activ... | 1 | 1 | 3.0% | 0.00433 | 130861 |
[ |
MDL1 | Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of pe... | 1 | 1 | 3.0% | 0.0073 | 75950 |
[ |
Reverse_YOL087C | YOL087C SGDID:S000005447, Chr XV from 158636-155286, reverse complement, Uncharacterized ORF, "Hypot... | 1 | 1 | 2.9% | 0.00454 | 125382 |
[ |
HSL7 | Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization ... | 1 | 1 | 2.7% | 0.00613 | 95153 |
[ |
SPC72 | Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... | 1 | 1 | 2.7% | 0.00816 | 72105 |
[ |
RAT1 | Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snR... | 1 | 1 | 2.4% | 0.00504 | 115934 |
[ |
MSS11 | Transcription factor involved in regulation of invasive growth and starch degradation; controls the ... | 1 | 1 | 2.2% | 0.00669 | 85050 |
[ |
YCF1 | Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detox... | 1 | 1 | 2.0% | 0.00335 | 171120 |
[ |
VPS5 | Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartm... | 1 | 1 | 1.9% | 0.00752 | 76484 |
[ |
YLR413W | Putative protein of unknown function; YLR413W is not an essential gene | 1 | 1 | 1.8% | 0.00752 | 72698 |
[ |
AIP1 | Actin cortical patch component, interacts with the actin depolymerizing factor cofilin; required to ... | 1 | 1 | 1.5% | 0.00825 | 67326 |
[ |
PDR12 | Plasma membrane ATP-binding cassette (ABC) transporter, weak-acid-inducible multidrug transporter re... | 1 | 1 | 1.1% | 0.00336 | 171064 |
[ |
TRM3 | 2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at posit... | 1 | 1 | 1.1% | 0.00353 | 165047 |
[ |
ECM5 | Non-essential protein of unknown function, contains ATP/GTP-binding site motif A; null mutant exhibi... | 1 | 1 | 0.6% | 0.0036 | 162700 |