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Bait Protein: MLP2
Organism: Saccharomyces cerevisiae
Publication: Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561.
Comments: #06 Alpha Factor Prep3-TiO2 Phosphopeptide enrichment

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UNFILTERED Run Results: [filter]

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   MLP2 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 31 143 28.2% 0.0341 195140
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 22 94 45.5% 0.104 42271
[View Peptides]   NUD1 Component of the spindle pole body outer plaque, required for exit from mitosis 22 47 15.2% 0.0221 94104
[View Peptides]   SPC29 Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... 17 80 32.8% 0.126 29280
[View Peptides]   CNM67 Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... 16 31 27.2% 0.0213 67400
[View Peptides]   SPC110 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... 15 23 13.7% 0.00975 111782
[View Peptides]   BBP1 Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... 7 16 19.0% 0.0166 45384
[View Peptides]   BGL2 Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance 7 13 18.5% 0.0166 34119
[View Peptides]   BFA1 Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... 5 8 13.6% 0.00558 66087
[View Peptides]   FUS3 Mitogen-activated serine/threonine protein kinase involved in mating; phosphoactivated by Ste7p; sub... 5 21 7.4% 0.0238 40772
[View Peptides]   gi|2119236, gi|4... sp|P35527|K1CI_HUMAN Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9), gi|545257|gb|AAC60... 4 10 10.0% 0.00643 61987
[View Peptides]   YEF3 Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes b... 4 10 4.7% 0.00383 115945
[View Peptides]   RPN13 Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for t... 3 10 20.5% 0.0256 17902
[View Peptides]   RPS3 Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease ... 3 17 16.7% 0.0283 26503
[View Peptides]   TDH2 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tet... 3 5 14.8% 0.00602 35847
[View Peptides]   TDH3 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tet... 3 6 14.8% 0.00723 35747
[View Peptides]   NPL3 RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from n... 3 7 7.0% 0.00676 45407
[View Peptides]   KRI1 Essential nucleolar protein required for 40S ribosome biogenesis; physically and functionally intera... 3 14 2.4% 0.00948 68654
[View Peptides]   YIL002W-A Putative protein of unknown function; identified by expression profiling and mass spectrometry 2 15 60.9% 0.087 7729
[View Peptides]   NRL_1IKFL, Conta... owl|| Immunoglobulin igg1-kappa antibody fragment fab complexed With... 2 4 15.4% 0.00748 23527
[View Peptides]   Contaminant_S682... owl|S68241| immunoglobulin light chain (Mab13-1) - mouse (fragment) 2 4 15.1% 0.00734 23846
[View Peptides]   RPL13B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Ap; not essential f... 2 7 14.6% 0.0141 22525
[View Peptides]   NBP1 Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... 2 3 14.1% 0.00376 37354
[View Peptides]   RPL12B, RPL12A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b d... 2 3 13.9% 0.00727 17823
[View Peptides]   CDC14 Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR ... 2 5 12.3% 0.00363 61907
[View Peptides]   TUB4 Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of th... 2 14 9.7% 0.0118 52628
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 2 4 9.0% 0.0032 54545
[View Peptides]   ACT1 Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... 2 12 7.5% 0.0128 41690
[View Peptides]   RRB1 Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with ... 2 6 7.2% 0.0047 57261
[View Peptides]   NOP58 Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the ... 2 16 7.0% 0.0125 56956
[View Peptides]   PSH1 Nuclear protein, putative RNA polymerase II elongation factor; isolated as Pob3p/Spt16p-binding prot... 2 16 6.7% 0.0158 47068
[View Peptides]   PXR1 Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2... 2 6 6.3% 0.00886 31312
[View Peptides]   GAS3 Putative 1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall 2 3 5.5% 0.00229 56794
[View Peptides]   PGK1 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... 2 18 5.3% 0.0173 44738
[View Peptides]   VMA2 Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... 2 7 5.2% 0.00542 57749
[View Peptides]   ACF2 Intracellular beta-1,3-endoglucanase, expression is induced during sporulation; may have a role in c... 2 5 4.6% 0.00257 88403
[View Peptides]   DEF1 RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and prot... 2 24 4.2% 0.013 83973
[View Peptides]   GFA1 Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... 2 4 4.2% 0.00223 80047
[View Peptides]   BDF1 Protein involved in transcription initiation at TATA-containing promoters; associates with the basal... 2 7 3.8% 0.00408 76978
[View Peptides]   HSL7 Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization ... 2 4 3.3% 0.00193 95153
[View Peptides]   BMS1 GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites... 2 8 3.0% 0.00271 135571
[View Peptides]   YAR009C Retrotransposon TYA Gag and TYB Pol genes; in YARCTY1-1 TYB is mutant and probably non-functional 2 5 2.6% 0.00167 136732
[View Peptides]   AFR1 Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture d... 2 3 2.6% 0.00194 71364
[View Peptides]   YDR261C-D Retrotransposon TYA Gag and TYB Pol genes; in YDRCTY1-3 TYB is mutant and probably non-functional 2 5 1.9% 0.00125 181660
[View Peptides]   YJR027W Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198651
[View Peptides]   YDR316W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198526
[View Peptides]   YDR210C-D Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198838
[View Peptides]   YLR157C-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198544
[View Peptides]   YDR098C-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198594
[View Peptides]   YDR365W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198658
[View Peptides]   YER138C Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198561
[View Peptides]   YML039W Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198571
[View Peptides]   YGR038C-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198520
[View Peptides]   YLR227W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198404
[View Peptides]   YJR029W Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198616
[View Peptides]   YML045W Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198524
[View Peptides]   YGR027W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 5 1.8% 0.00114 198656
[View Peptides]   RPP2B Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... 1 11 43.6% 0.04 11050
[View Peptides]   TIM11 Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex re... 1 3 34.4% 0.0125 10875
[View Peptides]   CMD1 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... 1 8 25.2% 0.0218 16135
[View Peptides]   HTA1 Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of tw... 1 1 25.0% 0.00303 13989
[View Peptides]   HTA2 Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of tw... 1 1 25.0% 0.00303 13989
[View Peptides]   GRX5 Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; mitochondrial ... 1 1 16.7% 0.00267 16931
[View Peptides]   RPL22A Protein component of the large (60S) ribosomal subunit, has similarity to Rpl22Bp and to rat L22 rib... 1 3 14.0% 0.00992 13693
[View Peptides]   RPS26B Protein component of the small (40S) ribosomal subunit; nearly identical to Rps26Ap and has similari... 1 2 12.6% 0.00672 13447
[View Peptides]   RPS26A Protein component of the small (40S) ribosomal subunit; nearly identical to Rps26Bp and has similari... 1 2 12.6% 0.00672 13505
[View Peptides]   DED1 ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... 1 6 9.9% 0.00397 65553
[View Peptides]   RPS19B Protein component of the small (40S) ribosomal subunit, required for assembly and maturation of pre-... 1 6 9.7% 0.0167 15891
[View Peptides]   RPS19A Protein component of the small (40S) ribosomal subunit, required for assembly and maturation of pre-... 1 6 9.7% 0.0167 15917
[View Peptides]   BUD21 Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated... 1 1 8.9% 0.00187 24385
[View Peptides]   FPR4 Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes... 1 3 8.7% 0.00306 43903
[View Peptides]   YOR287C Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of ... 1 7 8.3% 0.00933 36125
[View Peptides]   YDJ1 Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein trans... 1 7 8.1% 0.00685 44671
[View Peptides]   PIL1 Primary component of eisosomes, which are large immobile cell cortex structures associated with endo... 1 5 8.0% 0.0059 38349
[View Peptides]   YPR148C Protein of unknown function that may interact with ribosomes, based on co-purification experiments; ... 1 1 7.8% 9.2E-4 49279
[View Peptides]   ALD6 Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; re... 1 2 7.6% 0.0016 54414
[View Peptides]   WTM1 Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes;... 1 2 7.6% 0.00183 48383
[View Peptides]   ASC1 G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 th... 1 3 6.6% 0.00376 34805
[View Peptides]   ARB1 ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has sim... 1 4 6.2% 0.00262 68377
[View Peptides]   RPN8 Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 prot... 1 1 6.2% 0.00118 38313
[View Peptides]   PDI1 Protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, es... 1 6 5.9% 0.0046 58227
[View Peptides]   RPL10 Protein component of the large (60S) ribosomal subunit, responsible for joining the 40S and 60S subu... 1 1 5.9% 0.00181 25361
[View Peptides]   RPL5 Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 rib... 1 1 5.7% 0.00135 33715
[View Peptides]   MRH1 Protein that localizes primarily to the plasma membrane, also found at the nuclear envelope; the aut... 1 1 5.6% 0.00125 36191
[View Peptides]   CCT5 Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of... 1 1 5.3% 7.12E-4 61914
[View Peptides]   RPL7A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl7Bp and has similarit... 1 1 5.3% 0.00164 27638
[View Peptides]   RPL7B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl7Ap and has similarit... 1 1 5.3% 0.00164 27696
[View Peptides]   RVB1 Essential protein involved in transcription regulation; component of chromatin remodeling complexes;... 1 1 5.2% 8.64E-4 50453
[View Peptides]   GRS1 Cytoplasmic and mitochondrial glycyl-tRNA synthase that ligates glycine to the cognate anticodon bea... 1 5 4.9% 0.003 75411
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 1 7 4.4% 0.00411 74468
[View Peptides]   PAB1 Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between th... 1 5 4.3% 0.00347 64344
[View Peptides]   PRT1 eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translati... 1 14 4.2% 0.00734 88130
[View Peptides]   RNR4 Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiti... 1 1 4.1% 0.00116 40055
[View Peptides]   ENO1 Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosph... 1 2 3.9% 0.00183 46816
[View Peptides]   ENO2 Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... 1 2 3.9% 0.00183 46914
[View Peptides]   DIG1 Regulatory protein of unknown function, constitutively-expressed, involved in the regulation of mati... 1 12 3.8% 0.0106 49356
[View Peptides]   SPC72 Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... 1 3 3.7% 0.00193 72105
[View Peptides]   NOP56 Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that ... 1 1 3.4% 7.94E-4 56864
[View Peptides]   MPS2 Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... 1 1 3.4% 0.00103 44585
[View Peptides]   PDA1 E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarbo... 1 3 3.3% 0.00286 46343
[View Peptides]   gi|34033, gi|71526 pir||KRHU3 keratin 13, type I, cytoskeletal, long splice form - human, gi|34033|emb|CAA32786.1| ker... 1 1 3.1% 8.73E-4 49644
[View Peptides]   FBA1 Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversio... 1 1 3.1% 0.00111 39621
[View Peptides]   LSB3 Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldric... 1 1 2.9% 8.87E-4 48547
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 1 8 2.8% 0.00349 99619
[View Peptides]   NOP1 Nucleolar protein, component of the small subunit processome complex, which is required for processi... 1 1 2.8% 0.00122 34465
[View Peptides]   PMA2 Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator ... 1 8 2.7% 0.00338 102172
[View Peptides]   CTS1 Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 ... 1 2 2.7% 0.00142 59015
[View Peptides]   SSA2 ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shoc... 1 1 2.7% 6.26E-4 69470
[View Peptides]   SEC2 Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle tr... 1 1 2.6% 5.27E-4 84652
[View Peptides]   SSA1 ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... 1 1 2.6% 6.23E-4 69768
[View Peptides]   Reverse_IPI:IPI0... null, Ubiquitin-activating enzyme E1 homolog (D8), (NM_003335) ubiquitin-activating enzyme E1-like; ... 1 1 2.0% 3.96E-4 111720
[View Peptides]   SSB1 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... 1 1 2.0% 6.53E-4 66602
[View Peptides]   SSB2 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... 1 1 2.0% 6.53E-4 66595
[View Peptides]   Reverse_YBR198C TAF5 SGDID:S000000402, Chr II from 618518-616122, reverse complement, Verified ORF, "Subunit (90 kDa... 1 1 1.9% 5.01E-4 88968
[View Peptides]   IFH1 Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promo... 1 1 1.8% 3.69E-4 122491
[View Peptides]   FKS1 Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... 1 2 1.5% 4.26E-4 214849
[View Peptides]   ACC1 Acetyl-CoA carboxylase, biotin containing enzyme that catalyzes the carboxylation of acetyl-CoA to f... 1 7 0.9% 0.00125 250351
YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle