Bait Protein: | MLP2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561. |
Comments: | #22 Asynchronous Prep2-IMAC Phosphopeptide enrichment B1-2 |
Raw Data: |
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UNFILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
CNM67 | Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... | 10 | 14 | 17.4% | 0.0794 | 67400 |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 8 | 9 | 32.5% | 0.0817 | 42271 |
[ |
MLP2 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 7 | 8 | 7.9% | 0.0157 | 195140 |
[ |
CMD1 | Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... | 5 | 7 | 49.7% | 0.157 | 16135 |
[ |
SPC29 | Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... | 5 | 5 | 15.8% | 0.0651 | 29280 |
[ |
NUD1 | Component of the spindle pole body outer plaque, required for exit from mitosis | 5 | 6 | 8.7% | 0.0232 | 94104 |
[ |
CDC31 | Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... | 3 | 3 | 46.6% | 0.0614 | 18751 |
[ |
YIL002W-A | Putative protein of unknown function; identified by expression profiling and mass spectrometry | 2 | 2 | 39.1% | 0.0955 | 7729 |
[ |
BBP1 | Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... | 2 | 2 | 7.3% | 0.0171 | 45384 |
[ |
CDC14 | Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR ... | 2 | 2 | 6.9% | 0.012 | 61907 |
[ |
SPC110 | Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... | 2 | 2 | 4.2% | 0.00698 | 111782 |
[ |
SPC72 | Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... | 2 | 2 | 4.0% | 0.0106 | 72105 |
[ |
HHT1, HHT2 | Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated t... | 1 | 1 | 23.5% | 0.0242 | 15356 |
[ |
HTA1 | Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of tw... | 1 | 1 | 22.0% | 0.025 | 13989 |
[ |
HTA2 | Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of tw... | 1 | 1 | 22.0% | 0.025 | 13989 |
[ |
PAU20 | Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelome... | 1 | 1 | 19.2% | 0.0274 | 12877 |
[ |
RHR2 | Constitutively expressed isoform of DL-glycerol-3-phosphatase; involved in glycerol biosynthesis, in... | 1 | 1 | 10.3% | 0.0122 | 30439 |
[ |
RPL5 | Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 rib... | 1 | 1 | 9.4% | 0.0111 | 33715 |
[ |
Reverse_YLR375W | STP3 SGDID:S000004367, Chr XII from 871696-872727, Uncharacterized ORF, "Zinc-finger protein of unkn... | 1 | 1 | 9.3% | 0.00957 | 37718 |
[ |
YDJ1 | Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein trans... | 1 | 1 | 8.1% | 0.00805 | 44671 |
[ |
NSR1 | Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ri... | 1 | 1 | 7.7% | 0.00796 | 44535 |
[ |
WSS1 | Sumoylated protein of unknown function, identified based on genetic interactions with SMT3; UV-sensi... | 1 | 1 | 6.7% | 0.0122 | 30627 |
[ |
BFA1 | Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... | 1 | 1 | 6.4% | 0.00574 | 66087 |
[ |
PGK1 | 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... | 1 | 1 | 5.5% | 0.00792 | 44738 |
[ |
PDC1 | Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... | 1 | 1 | 5.3% | 0.00585 | 61495 |
[ |
OPI3 | Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two ... | 1 | 1 | 5.3% | 0.016 | 23150 |
[ |
PDC6 | Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino ... | 1 | 2 | 5.2% | 0.0117 | 61581 |
[ |
NMD3 | Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter... | 1 | 1 | 5.2% | 0.00636 | 59095 |
[ |
CCW14 | Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall | 1 | 3 | 4.2% | 0.0415 | 23268 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 1 | 1 | 4.0% | 0.00659 | 54545 |
[ |
ENO1 | Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosph... | 1 | 1 | 3.9% | 0.00754 | 46816 |
[ |
ENO2 | Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... | 1 | 1 | 3.9% | 0.00754 | 46914 |
[ |
TUB3 | Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form... | 1 | 1 | 3.8% | 0.0074 | 49694 |
[ |
TUB1 | Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form... | 1 | 1 | 3.8% | 0.00737 | 49800 |
[ |
Reverse_YNL164C | IBD2 SGDID:S000005108, Chr XIV from 326322-325267, reverse complement, Verified ORF, "Component of t... | 1 | 1 | 3.4% | 0.00936 | 40016 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 1 | 1 | 3.3% | 0.00719 | 50033 |
[ |
Reverse_YGL133W | ITC1 SGDID:S000003101, Chr VII from 257712-261506, Verified ORF, "Component of the ATP-dependent Isw... | 1 | 1 | 3.2% | 0.0026 | 145642 |
[ |
IXR1 | Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (hig... | 1 | 1 | 2.8% | 0.00552 | 67856 |
[ |
DSE4 | Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the d... | 1 | 1 | 2.5% | 0.00295 | 121064 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 1 | 1 | 2.2% | 0.00359 | 99619 |
[ |
ECM27 | Non-essential protein of unknown function | 1 | 1 | 2.2% | 0.00454 | 80657 |
[ |
RTG2 | Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcri... | 1 | 1 | 2.2% | 0.0056 | 65572 |
[ |
PMA2 | Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator ... | 1 | 1 | 2.1% | 0.00348 | 102172 |
[ |
Reverse_YGL066W | SGF73 SGDID:S000003034, Chr VII from 377612-379585, Verified ORF, "Probable 73 kDa subunit of SAGA h... | 1 | 1 | 2.1% | 0.00501 | 72878 |
[ |
TRM82 | Subunit of a tRNA methyltransferase complex composed of Trm8p and Trm82p that catalyzes 7-methylguan... | 1 | 1 | 1.8% | 0.00742 | 50488 |
[ |
BNI1 | Formin, nucleates the formation of linear actin filaments, involved in cell processes such as buddin... | 1 | 1 | 1.5% | 0.00169 | 219701 |
[ |
BBC1 | Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las1... | 1 | 1 | 1.5% | 0.00285 | 128296 |
[ |
Reverse_YKR064W | YKR064W SGDID:S000001772, Chr XI from 562189-564780, Uncharacterized ORF, "Putative protein of unkno... | 1 | 1 | 1.5% | 0.00381 | 101825 |
[ |
Reverse_YJR092W | BUD4 SGDID:S000003852, Chr X from 598649-602995, Verified ORF, "Protein involved in bud-site selecti... | 1 | 1 | 1.3% | 0.00227 | 165023 |
[ |
CAF130 | Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in control... | 1 | 1 | 1.2% | 0.00294 | 128780 |
[ |
Reverse_YPR159W | KRE6 SGDID:S000006363, Chr XVI from 857579-859741, Verified ORF, "Protein required for beta-1,6 gluc... | 1 | 1 | 1.0% | 0.00457 | 80123 |
[ |
Reverse_YML065W | ORC1 SGDID:S000004530, Chr XIII from 142210-144954, Verified ORF, "Largest subunit of the origin rec... | 1 | 1 | 0.8% | 0.0036 | 104400 |
[ |
BRR2 | RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in nativ... | 1 | 1 | 0.5% | 0.00152 | 246183 |