Bait Protein: | ASF1 |
Organism: | Saccharomyces cerevisiae |
Publication: | Green EM, Antczak AJ, Bailey AO, Franco AA, Wu KJ, Yates JR 3rd, Kaufman PD. Replication-independent histone deposition by the HIR complex and Asf1. Curr Biol. 2005 Nov 22;15(22):2044-9. |
Comments: | sample 3928 - Asf1-S-TEV-ZZ (in hir3delta mutant) |
Raw Data: |
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UNFILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
gi|265412 | gi|265412|gb|AAB25337.1| V8 protease [Staphylococcus aureus, Peptide, 276 aa] | 104 | 1293 | 89.5% | 0.719 | 29990 |
[ |
ASF1 | Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein t... | 38 | 118 | 56.6% | 0.0649 | 31603 |
[ |
EFB1 | Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP fo... | 9 | 11 | 39.3% | 0.00819 | 22627 |
[ |
TDH3 | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tet... | 13 | 19 | 39.2% | 0.00878 | 35747 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 20 | 47 | 37.3% | 0.0157 | 50033 |
[ |
RPS14A | Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processin... | 6 | 7 | 37.2% | 0.00784 | 14537 |
[ |
RPL31A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Bp and has similari... | 4 | 4 | 37.2% | 0.00543 | 12953 |
[ |
RPL31B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Ap and has similari... | 4 | 4 | 37.2% | 0.00543 | 12967 |
[ |
RPS14B | Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processin... | 6 | 7 | 37.0% | 0.00778 | 14650 |
[ |
RAD53 | Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autopho... | 26 | 31 | 36.9% | 0.00579 | 91962 |
[ |
RPS7A | Protein component of the small (40S) ribosomal subunit, nearly identical to Rps7Bp; interacts with K... | 6 | 6 | 34.7% | 0.00485 | 21622 |
[ |
gi|2119236, gi|4... | sp|P35527|K1CI_HUMAN Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9), gi|545257|gb|AAC60... | 13 | 20 | 33.3% | 0.00493 | 61987 |
[ |
RPP1A | Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation facto... | 2 | 5 | 32.1% | 0.00724 | 10908 |
[ |
RPL4B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Ap and has similarit... | 7 | 8 | 31.8% | 0.00339 | 39062 |
[ |
SSB1 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 14 | 18 | 30.0% | 0.00451 | 66602 |
[ |
CDC33 | Cytoplasmic mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex ... | 5 | 5 | 28.6% | 0.0036 | 24254 |
[ |
MLC1 | Essential light chain for Myo1p, light chain for Myo2p; stabilizes Myo2p by binding to the neck regi... | 2 | 2 | 27.5% | 0.00206 | 16444 |
[ |
RPL4A | N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to ... | 6 | 7 | 27.3% | 0.00297 | 39092 |
[ |
contaminant_NRL_... | owl|| Immunoglobulin g1 (igg1) (mcg) with a hinge deletion, chain H... | 10 | 19 | 25.5% | 0.00681 | 46852 |
[ |
RPL23B, RPL23A | Protein component of the large (60S) ribosomal subunit, identical to Rpl23Ap and has similarity to E... | 3 | 4 | 25.5% | 0.00448 | 14473 |
[ |
RPS4B, RPS4A | Protein component of the small (40S) ribosomal subunit; identical to Rps4Ap and has similarity to ra... | 5 | 6 | 24.9% | 0.00353 | 29410 |
[ |
SSB2 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 12 | 16 | 24.5% | 0.00401 | 66595 |
[ |
KRT10 | keratin 10 | 12 | 17 | 24.1% | 0.0044 | 59519 |
[ |
Contaminant_KERA... | 15 | 19 | 23.5% | 0.00453 | 65494 | |
[ |
RPS15 | Protein component of the small (40S) ribosomal subunit; has similarity to E. coli S19 and rat S15 ri... | 2 | 3 | 22.5% | 0.00324 | 16002 |
[ |
STM1 | Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with r... | 6 | 7 | 22.0% | 0.00393 | 29995 |
[ |
RPS8B, RPS8A | Protein component of the small (40S) ribosomal subunit; identical to Rps8Ap and has similarity to ra... | 3 | 3 | 22.0% | 0.0023 | 22490 |
[ |
DCD1 | Deoxycytidine monophosphate (dCMP) deaminase required for dCTP and dTTP synthesis; expression is NOT... | 2 | 2 | 20.5% | 9.84E-4 | 35646 |
[ |
RPS7B | Protein component of the small (40S) ribosomal subunit, nearly identical to Rps7Ap; interacts with K... | 3 | 3 | 20.0% | 0.00242 | 21634 |
[ |
RPS18B, RPS18A | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Ap and has similari... | 3 | 3 | 19.9% | 0.00315 | 17038 |
[ |
RPS19B | Protein component of the small (40S) ribosomal subunit, required for assembly and maturation of pre-... | 3 | 4 | 19.4% | 0.00426 | 15891 |
[ |
RPS19A | Protein component of the small (40S) ribosomal subunit, required for assembly and maturation of pre-... | 3 | 4 | 19.4% | 0.00426 | 15917 |
[ |
TDH2 | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tet... | 5 | 5 | 19.0% | 0.00231 | 35847 |
[ |
RPL12B, RPL12A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b d... | 3 | 3 | 18.2% | 0.00279 | 17823 |
[ |
RPL10 | Protein component of the large (60S) ribosomal subunit, responsible for joining the 40S and 60S subu... | 2 | 3 | 18.1% | 0.00208 | 25361 |
[ |
IBI2 | Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion p... | 2 | 2 | 17.8% | 0.00156 | 22179 |
[ |
SRO9 | Cytoplasmic RNA-binding protein that associates with translating ribosomes; involved in heme regulat... | 4 | 4 | 17.1% | 0.00141 | 48060 |
[ |
RPS13 | Protein component of the small (40S) ribosomal subunit; has similarity to E. coli S15 and rat S13 ri... | 2 | 2 | 16.6% | 0.00203 | 17029 |
[ |
KRT2 | keratin 2 | 6 | 6 | 16.6% | 0.00143 | 65865 |
[ |
VMA2 | Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... | 5 | 5 | 16.4% | 0.00148 | 57749 |
[ |
RPL17B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Ap and has similari... | 2 | 2 | 15.8% | 0.00167 | 20551 |
[ |
RPL17A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Bp and has similari... | 2 | 2 | 15.8% | 0.00167 | 20549 |
[ |
contaminant_NRL_... | owl|| Immunoglobulin g1 (igg1) (mcg) with a hinge deletion, chain L... | 2 | 2 | 13.9% | 0.00142 | 22815 |
[ |
RPL14B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl14Ap and has similari... | 2 | 2 | 13.8% | 0.00222 | 15153 |
[ |
RPL14A | N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to ... | 2 | 2 | 13.8% | 0.00222 | 15167 |
[ |
YDJ1 | Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein trans... | 5 | 5 | 13.2% | 0.00188 | 44671 |
[ |
RPS25B | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps25Ap and has similari... | 2 | 4 | 13.0% | 0.00568 | 12009 |
[ |
RPS25A | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps25Bp and has similari... | 2 | 4 | 13.0% | 0.00568 | 12039 |
[ |
ARC1 | Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering t... | 3 | 3 | 12.8% | 0.00122 | 42084 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 5 | 6 | 12.1% | 0.001 | 99619 |
[ |
YHR020W | Protein of unknown function that may interact with ribosomes, based on co-purification experiments; ... | 5 | 5 | 11.9% | 0.00112 | 77386 |
[ |
gi|34069 | gi|34069|emb|CAA24760.1| unnamed protein product [Homo sapiens] | 3 | 4 | 10.4% | 0.00172 | 39219 |
[ |
RPS2 | Protein component of the small (40S) subunit, essential for control of translational accuracy; phosp... | 3 | 3 | 10.2% | 0.00181 | 27450 |
[ |
DED1 | ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... | 3 | 4 | 10.1% | 0.00102 | 65553 |
[ |
KRT5 | keratin 5 | 4 | 5 | 9.8% | 0.0013 | 62461 |
[ |
NPL3 | RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from n... | 2 | 3 | 9.4% | 0.00111 | 45407 |
[ |
RPS24A, RPS24B | Protein component of the small (40S) ribosomal subunit; identical to Rps24Bp and has similarity to r... | 2 | 2 | 8.9% | 0.00227 | 15329 |
[ |
SSZ1 | Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that ... | 3 | 3 | 8.7% | 8.56E-4 | 58238 |
[ |
AZR1 | Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole dr... | 2 | 2 | 8.6% | 5.01E-4 | 67169 |
[ |
NSR1 | Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ri... | 3 | 3 | 8.2% | 0.00111 | 44535 |
[ |
RPS1B | Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Ap and has similarit... | 2 | 2 | 8.2% | 0.0012 | 28812 |
[ |
RPS1A | Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Bp and has similarit... | 2 | 2 | 8.2% | 0.0012 | 28743 |
[ |
SSA2 | ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shoc... | 2 | 2 | 8.0% | 4.8E-4 | 69470 |
[ |
SSA1 | ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... | 2 | 2 | 7.9% | 4.78E-4 | 69768 |
[ |
RPS23B, RPS23A | Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accurac... | 2 | 2 | 7.6% | 0.00212 | 16038 |
[ |
PMA2 | Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator ... | 3 | 4 | 7.5% | 6.48E-4 | 102172 |
[ |
SLX8 | Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachm... | 2 | 3 | 7.3% | 0.00168 | 30764 |
[ |
BFR2 | Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multi... | 2 | 2 | 7.1% | 5.75E-4 | 61203 |
[ |
AVT4 | Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seve... | 2 | 2 | 6.6% | 4.3E-4 | 80026 |
[ |
GFA1 | Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... | 2 | 2 | 6.4% | 4.28E-4 | 80047 |
[ |
HIR2 | Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene tra... | 3 | 3 | 6.3% | 5.26E-4 | 98445 |
[ |
DBP2 | Essential ATP-dependent RNA helicase of the DEAD-box protein family, involved in nonsense-mediated m... | 2 | 2 | 5.5% | 5.62E-4 | 60999 |
[ |
contaminant_KERA... | 2 | 2 | 4.8% | 5.46E-4 | 59822 | |
[ |
YEF3 | Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes b... | 2 | 2 | 3.0% | 2.94E-4 | 115945 |
[ |
KRT3 | keratin 3 | 2 | 5 | 2.1% | 0.00122 | 64511 |
[ |
YLR001C | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purifi... | 2 | 2 | 1.3% | 3.56E-4 | 97618 |